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81.
Debanu Das Robert D. Finn Polat Abdubek Tamara Astakhova Herbert L. Axelrod Constantina Bakolitsa Xiaohui Cai Dennis Carlton Connie Chen Hsiu‐Ju Chiu Michelle Chiu Thomas Clayton Marc C. Deller Lian Duan Kyle Ellrott Carol L. Farr Julie Feuerhelm Joanna C. Grant Anna Grzechnik Gye Won Han Lukasz Jaroszewski Kevin K. Jin Heath E. Klock Mark W. Knuth Piotr Kozbial S. Sri Krishna Abhinav Kumar Winnie W. Lam David Marciano Mitchell D. Miller Andrew T. Morse Edward Nigoghossian Amanda Nopakun Linda Okach Christina Puckett Ron Reyes Henry J. Tien Christine B. Trame Henry van den Bedem Dana Weekes Tiffany Wooten Qingping Xu Andrew Yeh Jiadong Zhou Keith O. Hodgson John Wooley Marc‐André Elsliger Ashley M. Deacon Adam Godzik Scott A. Lesley Ian A. Wilson 《Protein science : a publication of the Protein Society》2010,19(11):2131-2140
Sufu (Suppressor of Fused), a two‐domain protein, plays a critical role in regulating Hedgehog signaling and is conserved from flies to humans. A few bacterial Sufu‐like proteins have previously been identified based on sequence similarity to the N‐terminal domain of eukaryotic Sufu proteins, but none have been structurally or biochemically characterized and their function in bacteria is unknown. We have determined the crystal structure of a more distantly related Sufu‐like homolog, NGO1391 from Neisseria gonorrhoeae, at 1.4 Å resolution, which provides the first biophysical characterization of a bacterial Sufu‐like protein. The structure revealed a striking similarity to the N‐terminal domain of human Sufu (r.m.s.d. of 2.6 Å over 93% of the NGO1391 protein), despite an extremely low sequence identity of ~15%. Subsequent sequence analysis revealed that NGO1391 defines a new subset of smaller, Sufu‐like proteins that are present in ~200 bacterial species and has resulted in expansion of the SUFU (PF05076) family in Pfam. 相似文献
82.
Altay Burak Dalan Bahar Toptaş Zehra Buğra Nihat Polat Hülya Yılmaz-Aydoğan Arif Çimen Turgay İsbir 《Molecular biology reports》2013,40(8):5143-5149
Atherosclerosis is a major pathological process related with several important adverse vascular events including coronary artery disease, stroke, and peripheral arterial disease. Endothelial lipase is an enzyme the activity of which affects all of lipoproteins, whereas HDL is the main substrate. The purpose of our study was to investigate the effects of endothelial lipase gene polymorphism and inflammation markers (CRP, IL-1β, IL-6, IL-8 and TNF-α) in the atherosclerosis. 104 patients with atherosclerosis and 76 healthy individuals were included in the study. LIPG ?584C/T polymorphism gene polymorphisms were assessed with PCR–RFLP method. The serum CRP levels were measured by turbidimetric method using a biochemistry autoanalyzer, whereas serum IL-1β, IL-6, IL-8, TNF-α levels were determined by enzyme-linked immunosorbent assay. In this study, we found that the frequencies of TC genotype are more prevalent in patients than controls. We found a statistically significant difference of IL-6 levels between patient and control group. Our findings suggest that T allele might play a potential role in the susceptibility to atherogenesis in the Turkish population. 相似文献
83.
Sayad Kocahan Kubra Akillioglu Secil Binokay Leman Sencar Sait Polat 《Neurochemical research》2013,38(5):989-996
The N-Methyl-d-Aspartate (NMDA) receptor is expressed abundantly in the brain and plays an important role in neuronal development, learning and memory, neurodegenerative diseases, and neurogenesis. In this study, we evaluated the effects of NMDA receptor blockade during the early neurodevelopmental period on exploratory locomotion, anxiety-like behaviors and cognitive functions of adolescent Wistar rats. NMDA receptor hypofunction was induced 7–10 days after birth using MK-801 in rats (0.25 mg/kg twice a day for 4 days via intraperitoneal injection). The open-field (OF), elevated plus maze (EPM) and passive avoidance (PA) tests were used to evaluate exploratory locomotion, anxiety-like behaviors and cognitive functions. In the OF test, MK-801 caused an increase in locomotion behavior (p < 0.01) and in the frequency of rearing (p < 0.05). In the EPM test, MK-801 treatment increased the time spent in the open arms, the number of open arm entries and the amount of head dipping (p < 0.01). MK-801 treatment caused no statistical difference compared to the control group in the PA test (p > 0.05). Chronic NMDA receptor blockade during the critical period of maturation for the glutamatergic brain system (postnatal days 7–10) produces locomotor hyperactivity and decreased anxiety levels, but has no significant main effect on cognitive function during adolescence. 相似文献
84.
Akbas E Polat S Karakas-Celik S Altintas ZM Yildirim M Yilgor E 《Genetic counseling (Geneva, Switzerland)》2011,22(4):417-423
We report on a twenty-two months old male patient with hypotonia, mental and motor retardation and trigonocephaly. Standard GTG banding chromosomal analysis (from metaphyses of a periferal blood lymphocyte culture) showed 46,XY, der(9) monosomy 9pter-->p22, trisomy 10q26--> qter karyotype. This unbalanced translocation resulted from the father's t(9,10) (p22;p26) karyotype. Deletions of the terminal part of 9p and partial trisomy of chromosome 10q are rare chromosomal disorders. To our knowledge, this is the first case report in the literature of a deletion of 9pter-->p22.3 and a duplication of 10q26-->qter. We assume that the clinical anomalies are due to der(9) monosomy 9pter-->p22, trisomy 10q-->26qter. 相似文献
85.
Killer cell immunoglobulin-like receptors (KIRs) are a family of inhibitory and activating receptors expressed by natural
killer (NK) cells and regulate NK cells’ activity. KIR genes are highly polymorphic markers, characterized by a wide diversity,
and can therefore be considered as good population genetic markers. The aim of this study was to determine KIR gene frequencies,
ratios of haplotypes and genotypes in Southern Turkey and also to compare the data with other worldwide populations studied
previously. The study group consisted of 200 non-related individuals from Southern Turkey. The percentage of each KIR gene
in the population group was determined by direct counting. Differences between populations in the distribution of each KIR
gene and genotype profile were estimated by two-tailed Fisher Exact test. The most frequent non-framework KIR genes detected
in Southern Turkey population were: KIR 2DL1 (97%), KIR 3DL1 (91%), KIR 2DS4 (92%) and the pseudogene 2DP1 (96%). Fourty different
genotypes were found in 200 subjects and AA1 genotype was the most frequent (27%). Among 40 different genotypes, ten of these
were described for the first time in this study and were added to the database () numerized as genotype ID from 400 to 409. Gene frequencies and found genotypes demonstrated similarity of Southern Turkey’s
KIR repertoire with the KIR repertoires of Middle East and European population. High variability seen in KIR genome in this
region is thought to be formed as a result of migration and settlement of different civilizations in this region and heterogenity
formed in time. 相似文献
86.
Sare Sipal Zekai Halici ?lhami Kiki Beyzagul Polat Abdulmecit Albayrak Fatih Albayrak Emre Karakus Selina Aksak Berna Ozturk Cemal Gundogdu 《Journal of molecular histology》2012,43(6):723-735
The presence of liver disease in patients with progressively worsening insulin resistance may not be recognized until patients develop manifestations of the metabolic syndrome such as diabetes, hypertension, hyperlipidemia, and vascular disease. It was aimed to investigate whether three angiotensin II type 1 receptor antagonists (ARBs) (olmesartan, losartan, and valsartan) had preventive effect against hepatic fibrosis and this was a common characteristic among ARBs. In current study, 25 adult male rats were used and divided into five groups: the non-diabetic healthy group, alloxan induced diabetic (AID) control group, AID losartan group, AID valsartan group and AID olmesartan group. According to numerical density of hepatocytes, significant difference was found between the non-diabetic healthy group and diabetic control group. All treatments groups were significant when compared to diabetic control group. In diabetic control group it was examined swelling, irregular cristae arrangement in some of mitochondria. It was also determined mitochondria membrane degeneration in some areas of section profiles. In diabetic rats treated with losartan group, there were necrotic hepatocytes. In diabetic rats treated with valsartan group, predominantly, findings were similar to losartan group. In diabetic rats treated with olmesertan group, plates of hepatocytes were quite regular. There were hardly necrotic cells. Not only other organelles such as RER, SER and lysosom but also mitochondrial structures had normal appearance. In the diabetic control group electron microscopy revealed edema in both the cytoplasm and perinuclear area and the nuclear membranes appeared damaged. In conclusion, it was established that the most protective ARB the liver in diabetic rats was olmesartan, followed by losartan. 相似文献
87.
Knaapen Ad M. Schins Roel P.F. Polat Dünya Becker Andrea Borm Paul J.A. 《Molecular and cellular biochemistry》2002,(1):143-151
Reactive oxygen species (ROS) released by neutrophils have been suggested to play an important role in cancer development. Since the mechanisms underlying this effect in the respiratory tract are still unclear, we evaluated DNA damage induced by neutrophils in respiratory tract epithelial cells in vitro and in vivo. For in vitro studies, rat lung epithelial cells (RLE) were co-incubated with activated neutrophils, neutrophil-conditioned medium, or hydrogen peroxide. For in vivo studies, we considered the human nose as a target organ, comparing neutrophilic inflammation in the nasal lavage fluid with the oxidative DNA lesion 8-hydroxydeoxyguanosine (8-OHdG) in epithelial cells obtained by nasal brush. Our in vitro data show that human neutrophils are able to induce both 8-OHdG and strand breaks in DNA from RLE cells. Our data also suggest that DNA damage induced by neutrophils is inhibited when neutrophil-derived H2O2 is consumed by myeloperoxidase. In contrast, in the nose no association between neutrophil numbers and 8-OHdG was found. Therefore, it remains unclear whether neutrophils pose a direct genotoxic risk for the respiratory tract epithelium during inflammation, and more in vivo studies are needed to elucidate the possible association between neutrophils and genotoxicity in the lung. 相似文献
88.
Qingping Xu Sebastian Sudek Daniel McMullan Mitchell D. Miller Bernhard Geierstanger David H. Jones S. Sri Krishna Glen Spraggon Badry Bursalay Polat Abdubek Claire Acosta Eileen Ambing Tamara Astakhova Herbert L. Axelrod Dennis Carlton Jonathan Caruthers Hsiu-Ju Chiu Thomas Clayton Marc C. Deller Lian Duan Ian A. Wilson 《Structure (London, England : 1993)》2009,17(2):303-313
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90.
Kozbial P Xu Q Chiu HJ McMullan D Krishna SS Miller MD Abdubek P Acosta C Astakhova T Axelrod HL Carlton D Clayton T Deller M Duan L Elias Y Elsliger MA Feuerhelm J Grzechnik SK Hale J Han GW Jaroszewski L Jin KK Klock HE Knuth MW Koesema E Kumar A Marciano D Morse AT Murphy KD Nigoghossian E Okach L Oommachen S Reyes R Rife CL Spraggon G Trout CV van den Bedem H Weekes D White A Wolf G Zubieta C Hodgson KO Wooley J Deacon AM Godzik A Lesley SA Wilson IA 《Proteins》2008,71(3):1589-1596