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991.
Restoring exhausted Schiff's reagent 总被引:1,自引:0,他引:1
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Fu‐Jin Wei Lin‐Yun Kuang Hui‐Min Oung Sin‐Yuan Cheng Hshin‐Ping Wu Lin‐Tzu Huang Yi‐Tzu Tseng Wan‐Yi Chiou Vicki Hsieh‐Feng Cheng‐Han Chung Su‐May Yu Lan‐Ying Lee Stanton B. Gelvin Yue‐Ie C. Hsing 《The Plant journal : for cell and molecular biology》2016,85(5):648-659
Rice (Oryza sativa) is one of the world's most important crops. Rice researchers make extensive use of insertional mutants for the study of gene function. Approximately half a million flanking sequence tags from rice insertional mutant libraries are publicly available. However, the relationship between genotype and phenotype is very weak. Transgenic plant assays have been used frequently for complementation, overexpression or antisense analysis, but sequence changes caused by callus growth, Agrobacterium incubation medium, virulence genes, transformation and selection conditions are unknown. We used high‐throughput sequencing of DNA from rice lines derived from Tainung 67 to analyze non‐transformed and transgenic rice plants for mutations caused by these parameters. For comparison, we also analyzed sequence changes for two additional rice varieties and four T‐DNA tagged transformants from the Taiwan Rice Insertional Mutant resource. We identified single‐nucleotide polymorphisms, small indels, large deletions, chromosome doubling and chromosome translocations in these lines. Using standard rice regeneration/transformation procedures, the mutation rates of regenerants and transformants were relatively low, with no significant differences among eight tested treatments in the Tainung 67 background and in the cultivars Taikeng 9 and IR64. Thus, we could not conclusively detect sequence changes resulting from Agrobacterium‐mediated transformation in addition to those caused by tissue culture‐induced somaclonal variation. However, the mutation frequencies within the two publically available tagged mutant populations, including TRIM transformants or Tos17 lines, were about 10‐fold higher than the frequency of standard transformants, probably because mass production of embryogenic calli and longer callus growth periods were required to generate these large libraries. 相似文献
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Yeji Kim Kyungju Song Hwajin Lee Dohyun Kim Jintae Kim Minsub Chung 《Biotechnology and Bioprocess Engineering》2016,21(6):720-725
Far-red fluorescent proteins are beneficial for imaging in mammals. Here, starting from mCherry, the most commonly used among the different types of red fluorescent proteins (RFP), not having a H-bond network in its original form, we sought to recover the hydrogen bond network in mCherry. By comparing the structure of wtGFP and mCherry, we focused on a few key residues involved in a proton wire, and discovered an I197T mutant that showed a more red-shifted fluorescence. The detailed optical and photo-switching properties of related engineered RFPs are described. This study will guide further development of monomeric far-red FPs. 相似文献
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Nicola G. Ghazi Emad B. Abboud Sawsan R. Nowilaty Hisham Alkuraya Abdulrahman Alhommadi Huimin Cai Rui Hou Wen-Tao Deng Sanford L. Boye Abdulrahman Almaghamsi Fahad Al Saikhan Hassan Al-Dhibi David Birch Christopher Chung Dilek Colak Matthew M. LaVail Douglas Vollrath Kirsten Erger Wenqiu Wang Thomas Conlon Kang Zhang William Hauswirth Fowzan S. Alkuraya 《Human genetics》2016,135(3):327-343
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Volkan Okur Megan T. Cho Lindsay Henderson Kyle Retterer Michael Schneider Shannon Sattler Dmitriy Niyazov Meron Azage Sharon Smith Jonathan Picker Sharyn Lincoln Mark Tarnopolsky Lauren Brady Hans T. Bjornsson Carolyn Applegate Amy Dameron Rebecca Willaert Berivan Baskin Jane Juusola Wendy K. Chung 《Human genetics》2016,135(7):699-705