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971.
972.
Cryosections and whole-mount preparations of the guinea pig small intestine and colon were single or double immunolabeled
using the anti-c-Kit and protein gene product 9.5 antibodies. Immunolabeled specimens were observed under a confocal laser
scanning microscope. The main findings of the present study are: (1) the distribution and profiles of three-dimensional structures
of c-Kit-positive cellular networks in the small intestine and colon, and (2) the anatomical relations of c-Kit-positive cells
to the enteric nerves in the layers. In the small intestine, c-Kit-positive cellular networks were observed at levels of the
deep muscular plexus and myenteric plexus. The c-Kit-positive cellular networks ran along or overlay the nerve fibers at the
deep muscular plexus, while they showed the reticular structures intermingled with the nerve elements at the myenteric plexus.
In the colon, c-Kit-positive cellular networks were observed at levels of the submuscular plexus and myenteric plexus, and
were further identified within the circular and longitudinal muscle layers as well as in the subserosal layer. In the circular
muscle layer, c-Kit-positive cells surrounded the associated nerve fibers and extended several long processes toward the adjacent
c-Kit-positive cells. The c-Kit-positive cellular networks within the longitudinal muscle layer as well as in the subserosal
layer were not associated with the nerve fibers. In the layers of the intestinal wall with c-Kit-positive cells, the cellular
networks of the interstitial cells were identified in ultrastructure. The characteristic profiles of c-Kit-positive cellular
networks provide a morphological basis upon which to investigate the mechanisms regulating intestinal movement.
Received: 14 July 1998 / Accepted: 2 September 1998 相似文献
973.
Zeller R Haramis AG Zuniga A McGuigan C Dono R Davidson G Chabanis S Gibson T 《Cell and tissue research》1999,298(1):85-93
Formin was originally isolated as the gene affected by the murine limb deformity (ld) mutations, which disrupt the epithelial-mesenchymal interactions regulating patterning of the vertebrate limb autopod. More
recently, a rapidly growing number of genes with similarity to formin have been isolated from many different species including fungi and plants. Genetic and biochemical analysis shows that formin family members function in cellular processes regulating either cytokinesis and/or cell polarisation. Another common feature
among formin family members is their requirement in morphogenetic processes such as budding and conjugation of yeast, establishment of
Drosophila oocyte polarity and vertebrate limb pattern formation. Vertebrate formins are predominantly nuclear proteins which control polarising activity in limb buds through establishment of the SHH/FGF-4
feedback loop. Formin acts in the limb bud mesenchyme to induce apical ectodermal ridge (AER) differentiation and FGF-4 expression in the posterior
AER compartment. Finally, disruption of the epithelial-mesenchymal interactions controlling induction of metanephric kidneys
in ld mutant embryos indicates that formin might function more generally in transduction of morphogenetic signals during embryonic pattern formation.
Received: 24 September 1998 / Accepted: 30 September 1998 相似文献
974.
975.
976.
Organization of the human orphan nuclear receptor Nurr1 gene 总被引:5,自引:0,他引:5
977.
Characterization of an outer membrane protein gene, pgmA, and its gene product from Porphyromonas gingivalis 总被引:5,自引:0,他引:5
Hongo H Osano E Ozeki M Onoe T Watanabe K Honda O Tani H Nakamura H Yoshimura F 《Microbiology and immunology》1999,43(10):937-946
A gene upstream from fimA, the gene encoding fimbrilin, on the chromosome of Porphyromonas gingivalis was sequenced and shown to be the gene encoding an outer membrane protein in this organism based on homology and biochemical analyses. Therefore, the gene (formerly ORF5) was designated pgmA, the P. gingivalis outer membrane protein A gene. The gene product, PgmA, was sensitive to protease, and was detected as a 60-kDa protein from wild-type strains with trichloroacetic acid treatment, which was carried out to destroy intrinsic proteases, and from protease-deficient mutants without this treatment prior to electrophoresis. PgmA was indeed present in the membrane fraction. Its nature was determined to be that of outer membrane proteins in gram-negative bacteria based on attempts at differential extraction of inner membrane proteins with detergents. No evidence has been found thus far from functional analyses that this protein is related to fimbrial morphogenesis and functions or to serum resistance of this organism. 相似文献
978.
979.
The elucidation of the biological role of glycan is one of the most important issues to be resolved following the genome project. RNA interference is becoming an efficient reverse genetic tool for studying gene function in model organisms, including C.elegans and Drosophila melanogaster. Our molecular evolutionary study has shown that a prototype of glycosyltransferases, which synthesize a variety of glycan structures in the Golgi apparatus, was conserved between mammals and Drosophila. For analyses of the basic physiological functions of glycans, we established the Drosophila inducible RNAi knockdown system and applied it to one glycosyltransferase and one transporter, proteoglycan UDP-galactose: beta-xylose beta1,4galactosyltransferase I and the PAPS-transporter, respectively. If on the silencing of each gene induced ubiquitously under the control of a cytoplasmic actin promoter, the RNAi knockdown fly died, then the protein was indispensable for life. The expression of the target gene was disrupted specifically and the degree of interference was well correlated with the phenotype. The inducible RNAi knockdown fly obtained using the GAL4-UAS system will pave the way for the functional analysis of glycans. 相似文献
980.