Neutroperiodism in different varieties of groundnut with respect to zinc and its uptake as affected by phosphorus application

Summary A greenhouse experiment was conducted to study the Zn uptake at various growth stages of groundnut plant. Soil used for the study was non calcareous typic Torripsamment, low in available Zn. Results obtained revealed that highest amount of Zn was accumulated in shoot portion at midflowering and declined severely at 75 days of plant growth. Its concentration in shoot portion increased again at maturity. Maximum Zn translocation from the shoot portion to fruits occurred between 50 and 75 days growth period. P application showed an antagonistic effect on Zn uptake. re]19760213     

A waveguide imaging platform for live‐cell TIRF imaging of neurons over large fields of view

Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been demonstrated for short‐term live‐imaging of robust cell types [3‐5], but not yet for delicate primary neurons nor over extended periods of time. Here, we present a waveguide‐based TIRFM set‐up for live‐cell imaging of demanding samples. Using the developed microscope, referred to as the ChipScope, we demonstrate successful culturing and imaging of fibroblasts, primary rat hippocampal neurons and axons of Xenopus retinal ganglion cells (RGCs). The high contrast and gentle illumination mode provided by TIRFM coupled with the exceptionally large excitation areas and superior illumination homogeneity offered by photonic waveguides have potential for a wide application span in neuroscience applications.