全文获取类型
收费全文 | 1429篇 |
免费 | 90篇 |
国内免费 | 2篇 |
出版年
2023年 | 5篇 |
2022年 | 11篇 |
2021年 | 19篇 |
2020年 | 11篇 |
2019年 | 9篇 |
2018年 | 14篇 |
2017年 | 17篇 |
2016年 | 38篇 |
2015年 | 49篇 |
2014年 | 62篇 |
2013年 | 83篇 |
2012年 | 115篇 |
2011年 | 109篇 |
2010年 | 69篇 |
2009年 | 52篇 |
2008年 | 87篇 |
2007年 | 81篇 |
2006年 | 91篇 |
2005年 | 87篇 |
2004年 | 98篇 |
2003年 | 73篇 |
2002年 | 56篇 |
2001年 | 7篇 |
1999年 | 15篇 |
1998年 | 15篇 |
1997年 | 10篇 |
1996年 | 9篇 |
1995年 | 12篇 |
1994年 | 14篇 |
1993年 | 11篇 |
1991年 | 10篇 |
1990年 | 6篇 |
1989年 | 8篇 |
1988年 | 4篇 |
1987年 | 5篇 |
1986年 | 8篇 |
1985年 | 6篇 |
1984年 | 6篇 |
1982年 | 7篇 |
1981年 | 9篇 |
1980年 | 9篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1977年 | 6篇 |
1975年 | 4篇 |
1974年 | 5篇 |
1971年 | 5篇 |
1962年 | 5篇 |
1935年 | 4篇 |
1912年 | 4篇 |
排序方式: 共有1521条查询结果,搜索用时 15 毫秒
111.
112.
Mary Agnes Hamilton 《应用发育科学》2015,19(2):87-107
Social inventions are new ways of solving human problems. This article reports on an action research project designed to find social inventions to reduce structural lag in four programs that support the transition to adulthood of marginalized youth in Latin America. The investigators engaged youth and staff members in identifying important questions, collecting and interpreting data, and using findings to improve their practices. Their issues aligned with social setting features: activities, resources, roles, and norms. Among their social inventions were “the life project,” the role of mentor, youth responsibility, and new norms of reflection introduced by action research, which not only revealed social inventions but generated them as well. Interaction with external parties contributed to this process: the investigators, “local researchers” engaged as part of the project, participants in conferences convened for participants. Rather than recommending social inventions for adoption in other locations, we recommend using action research to generate local social inventions. 相似文献
113.
114.
iNKT cells, CD1d dependent natural killer T cells are a unique population of T cells. The capacity of iNKT cells to produce regulatory cytokines first provided an indication of their regulatory potential. Later on, in experimental models as well as in patients afflicted with an auto-immune disease, such as Type 1 diabetes mellitus, multiple sclerosis, and systemic lupus erythematosus along with others, a deficit in iNKT cell number was observed, suggesting the role these cells may possibly have in the prevention of auto-immune diseases. More importantly, experimental strategies which focused on increasing the volume or stimulation of iNKT cells in laboratory animals, demonstrated an improved level of protection against the development of auto-immune diseases. This article reviews the mechanism of protection against autoimmunity by iNKT cells, discusses the obstacles against and indications for the potential use of iNKT cell manipulation in the treatment of human auto-immune diseases. 相似文献
115.
Chen J Zhang X Cao R Lu X Zhao S Fekete A Huang Q Schmitt-Kopplin P Wang Y Xu Z Wan X Wu X Zhao N Xu C Xu G 《Journal of proteome research》2011,10(5):2625-2632
The aim of this study was to use a two steps strategy metabolomics to screen/identify and validate novel metabolic biomarker(s) for epithelial ovarian cancer (EOC). In the screening step, serum samples from 27 healthy women, 28 benign ovarian tumors, and 29 EOCs were analyzed by using LC-MS based nontargeted metabolomics. The three groups were separated with OSC filtered PLS-DA model, and six metabolites (27-nor-5β-cholestane-3,7,12,24,25 pentol glucuronide (CPG), phenylalanine, glycocholic acid, propionylcarnitine, Phe-Phe and Lyso PC (18:2)) were considered as potential biomarker candidates. In the validation step, the six metabolites were analyzed in targeted metabolomics by LC-selective ion monitoring mass spectrometry in another 685 serum samples with various clinical backgrounds. As a result, CPG was evaluated to be a potential biomarker and its content was elevated in EOC tissues compared with benign ovarian tumor tissues (p = 0.0005). Besides, CPG levels were found to be up-regulated in early stage EOC and in the three types of EOC histological types. Other variables such as nonovarian diseases, medicine consumption, gynecological inflammations, and menopausal state did not interfere in using CPG as diagnosis marker. CPG was found to be complementary to CA125. Our findings suggest that CPG can be considered a statistical relevant biomarker of EOC, ready for early stage detection. 相似文献
116.
Galajda Z Balla J Szentmiklosi AJ Biro T Czifra G Dobrosi N Cseppento A Patonay L Roszer T Balla G Popescu LM Lekli I Tosaki A 《Journal of cellular and molecular medicine》2011,15(12):2614-2623
The study has analysed the action of histamine in the rabbit venous system and evaluated its potential role in contraction during increased venous pressure. We have found that a great variety exists in histamine sensitivity and H(1) -histamine receptor expression in various types of rabbit veins. Veins of the extremities (saphenous vein, femoral vein, axillary vein) and abdomen (common iliac vein, inferior vena cava) responded to histamine by a prominent, concentration-dependent force generation, whereas great thoracic veins (subclavian vein, superior vena cavas, intrathoracic part of inferior vena cava) and a pelvic vein (external iliac vein) exhibited slight sensitivity to exogenous histamine. The lack of reactivity to histamine was not due to increased activity of nitric oxide synthase (NOS) or heme oxygenase-1. H(1) -histamine receptor expression of veins correlated well with the histamine-induced contractions. Voltage-dependent calcium channels mediated mainly the histamine-induced force generation of saphenous vein, whereas it did not act in the inferior vena cava. In contrast, the receptor-operated channels were not involved in this response in either vein. Tyrosine phosphorylation occurred markedly in response to histamine in the saphenous vein, but not in the inferior vena cava. Histamine induced a prominent ρ kinase activation in both vessels. Protein kinase C and mitogen-activated protein kinase (MAPK) were not implicated in the histamine-induced intracellular calcium sensitization. Importantly, transient clamping of the femoral vein in animals caused a short-term constriction, which was inhibited by H(1) -histamine receptor antagonist in vivo. Furthermore, a significantly greater histamine immunopositivity was detected in veins after stretching compared to the resting state. We conclude that histamine receptor density adapts to the actual requirements of the circulation, and histamine liberated by the venous wall during increased venous pressure contributes to the contraction of vessels, providing a force for the venous return. 相似文献
117.
118.
119.
Putters J da Silva Almeida AC van Kerkhof P van Rossum AG Gracanin A Strous GJ 《PloS one》2011,6(2):e14676
Background
Length and intensity of signal transduction via cytokine receptors is precisely regulated. Degradation of certain cytokine receptors is mediated by the ubiquitin ligase SCF(βTrCP). In several instances, Janus kinase (Jak) family members can stabilise their cognate cytokine receptors at the cell surface.Principal Findings
In this study we show in Hek293 cells that Jak2 binding to the growth hormone receptor prevents endocytosis in a non-catalytic manner. Following receptor activation, the detachment of phosphorylated Jak2 induces down-regulation of the growth hormone receptor by SCF(βTrCP). Using γ2A human fibroblast cells we show that both growth hormone-induced and constitutive growth hormone receptor endocytosis depend on the same factors, strongly suggesting that the modes of endocytosis are identical. Different Jak2 RNA levels in HepG2, IM9 and Hek293 cells indicate the importance of cellular concentration on growth hormone receptor function. Both Jak2 and βTrCP bind to neighbouring linear motifs in the growth hormone receptor tail without the requirement of modifications, indicating that growth hormone sensitivity is regulated by the cellular level of non-committed Jak2.Conclusions/Significance
As signal transduction of many cytokine receptors depends on Jak2, the study suggests an integrative role of Jak2 in cytokine responses based on its enzyme activity as well as its stabilising properties towards the receptors. 相似文献120.
Nguyen NQ Castermans K Berndt S Herkenne S Tabruyn SP Blacher S Lion M Noel A Martial JA Struman I 《PloS one》2011,6(11):e27318