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排序方式: 共有180条查询结果,搜索用时 241 毫秒
71.
Bryndan P. Durham Jana Grote Kerry A. Whittaker Sara J. Bender Haiwei Luo Sharon L. Grim Julia M. Brown John R. Casey Antony Dron Lennin Florez-Leiva Andreas Krupke Catherine M. Luria Aric H. Mine Olivia D. Nigro Santhiska Pather Agathe Talarmin Emma K. Wear Thomas S. Weber Jesse M. Wilson Matthew J. Church Edward F. DeLong David M. Karl Grieg F. Steward John M. Eppley Nikos C. Kyrpides Stephan Schuster Michael S. Rappé 《Standards in genomic sciences》2014,9(3):632-645
Strain HIMB11 is a planktonic marine bacterium isolated from coastal seawater in Kaneohe Bay, Oahu, Hawaii belonging to the ubiquitous and versatile Roseobacter clade of the alphaproteobacterial family Rhodobacteraceae. Here we describe the preliminary characteristics of strain HIMB11, including annotation of the draft genome sequence and comparative genomic analysis with other members of the Roseobacter lineage. The 3,098,747 bp draft genome is arranged in 34 contigs and contains 3,183 protein-coding genes and 54 RNA genes. Phylogenomic and 16S rRNA gene analyses indicate that HIMB11 represents a unique sublineage within the Roseobacter clade. Comparison with other publicly available genome sequences from members of the Roseobacter lineage reveals that strain HIMB11 has the genomic potential to utilize a wide variety of energy sources (e.g. organic matter, reduced inorganic sulfur, light, carbon monoxide), while possessing a reduced number of substrate transporters. 相似文献
72.
73.
Sawitree Nangola Agathe Urvoas Marie Valerio-Lepiniec Wannisa Khamaikawin Supachai Sakkhachornphop Saw-See Hong Pierre Boulanger Philippe Minard Chatchai Tayapiwatana 《Retrovirology》2012,9(1):1-27
Background
Ankyrins are cellular mediators of a number of essential protein-protein interactions. Unlike intrabodies, ankyrins are composed of highly structured repeat modules characterized by disulfide bridge-independent folding. Artificial ankyrin molecules, designed to target viral components, might act as intracellular antiviral agents and contribute to the cellular immunity against viral pathogens such as HIV-1.Results
A phage-displayed library of artificial ankyrins was constructed, and screened on a polyprotein made of the fused matrix and capsid domains (MA-CA) of the HIV-1 Gag precursor. An ankyrin with three modules named AnkGAG1D4 (16.5 kDa) was isolated. AnkGAG1D4 and MA-CA formed a protein complex with a stoichiometry of 1:1 and a dissociation constant of K d ~ 1 ??M, and the AnkGAG1D4 binding site was mapped to the N-terminal domain of the CA, within residues 1-110. HIV-1 production in SupT1 cells stably expressing AnkGAG1D4 in both N-myristoylated and non-N-myristoylated versions was significantly reduced compared to control cells. AnkGAG1D4 expression also reduced the production of MLV, a phylogenetically distant retrovirus. The AnkGAG1D4-mediated antiviral effect on HIV-1 was found to occur at post-integration steps, but did not involve the Gag precursor processing or cellular trafficking. Our data suggested that the lower HIV-1 progeny yields resulted from the negative interference of AnkGAG1D4-CA with the Gag assembly and budding pathway.Conclusions
The resistance of AnkGAG1D4-expressing cells to HIV-1 suggested that the CA-targeted ankyrin AnkGAG1D4 could serve as a protein platform for the design of a novel class of intracellular inhibitors of HIV-1 assembly based on ankyrin-repeat modules. 相似文献74.
Sandra Muschiol Staffan Normark Birgitta Henriques-Normark Agathe Subtil 《BMC microbiology》2009,9(1):75-7
Background
Chlamydiae are obligate intracellular pathogens that possess a type III secretion system to deliver proteins into the host cell during infection. Small molecule inhibitors of type III secretion in Yersinia, termed INPs (Innate Pharmaceuticals AB) were reported to strongly inhibit Chlamydia growth in epithelial cells. In this study we have analyzed the effect of these drugs on bacterial invasiveness. 相似文献75.
Clonal structure through space and time: High stability in the holothurian Stichopus chloronotus (Echinodermata)
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Agathe Pirog Pauline Gélin Alexandre Bédier Grégoire Bianchetti Stéphane Georget Patrick Frouin Hélène Magalon 《Ecology and evolution》2017,7(18):7534-7547
Sea cucumbers are increasingly exploited for human consumption and for their curative properties, and many wild populations are now depleted or in danger of extinction. While aquaculture is seen as an alternative to fisheries and as a mean to restore wild populations, more knowledge is needed on their reproductive strategies to render this practice efficient, notably for fissiparous holothurians, which are some of the mobile animals able of asexual reproduction by transverse fission. Little information is available on their population genetic diversity and structure. Here, the clonal structure of populations of the fissiparous sea cucumber Stichopus chloronotus has been investigated using nine microsatellite loci and a random sampling, at different spatial (intra‐reef and inter‐reef) and temporal (inter‐season and inter‐year) scales. Our findings highlight the importance of asexual reproduction in maintaining these populations, and the prevalence of the “initial seedling recruitment” strategy (ISR), leading to a high stability of clonal composition over seasons and years. It also seemed that clonal propagation was limited to the reef scale (<10 km) while reefs were connected by sexual dispersal. This is the first time that clonal structure in sea cucumbers has been studied at such a fine scale, with a specific sampling strategy. It provides key findings on the genetic diversity and structure of fissiparous sea cucumbers, which will be useful for the management of wild populations and aquaculture. 相似文献
76.
Agathe Pirog Hélène Magalon Thomas Poirout Sébastien Jaquemet 《Journal of fish biology》2019,95(5):1195-1206
To improve understanding of bull shark Carcharhinus leucas reproductive biology, we analysed reproductive traits from 118 bull sharks caught along Reunion Island coasts (Western Indian Ocean), including 16 gravid females. Specific microsatellite loci were used to investigate the frequency of multiple paternity. Males and females reached maturity at c. 234 cm and 257 cm total length (LT), respectively, and litter sizes ranged from 5 to 14 embryos. Analysis of the 16 litters collected in various months of the year indicated that parturition occurs between October and December, with a size at birth c. 60–80 cm LT and that the gestation period is probably c. 12 months. Assuming a 1 year resting period and a period of sperm storage (4–5 months) between mating (in June–September) and fertilisation, the reproductive cycle of bull sharks at Reunion Island would be biennial. At least 56.25% of the litters investigated were polyandrous, sired by 2–5 males. Several males that each sired several litters conceived during the same or distinct mating seasons were detected, suggesting both a seasonal aggregation of sharks to mate and some male fidelity to mating site. Altogether, these findings provide valuable information for both shark risk management and conservation of the species in the Western Indian Ocean. 相似文献
77.
Oumarou Traoré Issa Wonni Gilles Cellier Fousseni Boro Agathe Allibert Cyrille Tinlé Zombré Léonard Somgnogdin Ouédraogo Irénée Somda 《Journal of Phytopathology》2023,171(1):1-11
Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is a disease that negatively affects the cultivation of Solanaceae crops in Burkina Faso. Knowledge of the pathogen diversity is essential to deploy locally adapted control methods. In this study, diseased plants showing typical bacterial wilt symptoms were collected in the three main agroclimatic zones of Burkina Faso for the detection of RSSC isolates. Strain characterization was achieved through a phylogenetic and pathogenicity diversity assessment. A total of 102 isolates were sampled, and Phylotype I (Ralstonia pseudosolanacearum) was predominant (n = 101; sequevars 14, 31, 34, and 46). The remaining isolate was characterized as Phylotype IIA-35 (Ralstonia solanacearum). Phylotypes I-31 and I-46 were predominant and both characterized as the most the aggressive group of strains amongst a subset of 33 representative isolates. Our findings provide valuable information as regard RSSC diversity that breeders and resistance programme should target in order to fight this pathogen in Burkina Faso and around the world. 相似文献
78.
Agathe Garnier Ali Berredjem Bernard Botton 《Fungal genetics and biology : FG & B》1997,22(3):168-176
The NAD-dependent glutamate dehydrogenase (GDH) (EC 1.4.1.2) fromLaccaria bicolorwas purified 410-fold to apparent electrophoretic homogeneity with a 40% recovery through a three-step procedure involving ammonium sulfate precipitation, anion-exchange chromatography on DEAE–Trisacryl, and gel filtration. The molecular weight of the native enzyme determined by gel filtration was 470 kDa, whereas sodium dodecyl sulfate–polyacrylamide gel electrophoresis gave rise to a single band of 116 kDa, suggesting that the enzyme is composed of four identical subunits. The enzyme was specific for NAD(H). The pH optima were 7.4 and 8.8 for the amination and deamination reactions, respectively. The enzyme was found to be highly unstable, with virtually no activity after 20 days at −75°C, 4 days at 4°C, and 1 h at 50°C. The addition of ammonium sulfate improved greatly the stability of the enzyme and full activity was still observed after several months at −75°C. NAD-GDH activity was stimulated by Ca2+and Mg2+but strongly inhibited by Cu2+and slightly by the nucleotides AMP, ADP, and ATP. The Michaelis constants for NAD, NADH, 2-oxoglutarate, and ammonium were 282 μM, 89 μM, 1.35 mM, and 37 mM, respectively. The enzyme had a negative cooperativity for glutamate (Hill number of 0.3), and itsKmvalue increased from 0.24 to 3.6 mM when the glutamate concentration exceeded 1 mM. These affinity constants of the substrates, compared with those of the NADP-GDH of the fungus, suggest that the NAD-GDH is mainly involved in the catabolism of glutamate, while the NADP-GDH is involved in the catalysis of this amino acid. 相似文献
79.
Vincent Parinet Elise Chapiro Audrey Bidet Baptiste Gaillard Odile Maarek Laurence Simon Christine Lefebvre Sabine Defasque Marie-Joelle Mozziconacci Anne Quinquenel Matthieu Decamp François Lifermann Nadia Ali-Ammar Agathe Maillon Marine Baron Mélanie Martin Stéphanie Struski Dominique Penther Jean-Baptiste Micol Nathalie Auger Chrystèle Bilhou-Nabera Jean-Alain Martignoles Sylvie Tondeur Florence Nguyen-Khac Pierre Hirsch Damien Roos-Weil on behalf the FILO GFCH groups 《Journal of cellular and molecular medicine》2021,25(20):9557-9566
80.
T Bourgogne M J Vacheron M Guinand G Michel 《The International journal of biochemistry》1992,24(3):471-476
1. A gamma-D-glutamyl-L-di-amino acid endopeptidase II (EC3.4.-.-) active on the peptide moieties of some bacterial peptidoglycans has been purified to homogeneity from the sporulation medium and from the spores of Bacillus sphaericus. 2. Enzyme from both sources showed a single protein band (Mr 28,000) by polyacrylamide gel electrophoresis under denaturing conditions. It is an acidic protein (pI 4.1). Kinetic studies have shown a Km value of 0.24 mM and an apparent Vmax of 8.3 mumol min-1 mg-1 with the pentapeptide L-Ala-gamma-D-Glu-L-Lys-D-[14C]Ala-D-[14C]Ala as substrate. 3. The enzyme was inhibited by p-hydroxymercuribenzoate, a sulfhydryl inhibitor. 4. The 38-residue N-terminal region was sequenced. It may be useful to construct a nucleotide probe for the research of the gene encoding this enzyme. 相似文献