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161.
Creation and evolution of 30 years of the inferior pedicle in reduction mammaplasties 总被引:6,自引:0,他引:6
The article describes the experience of the senior author over the past 30 years using the glandular dermo-lipo flap. The flap primarily provides an effective enhancement of the shape of the breast that varies according to the age of the patient, skin elasticity, and mammary gland firmness. More than 2000 patients have had this procedure, including those with asymmetric breasts who had the procedure done on only one breast. 相似文献
162.
We determined the estrus profile (weaning-to-estrus interval (WEI), estrus duration (ED), and frequency of estrus per detection period) in 184 female swine and estimated the effect of the WEI, ED and frequency of artificial insemination (AI) on farrowing rate (FR) and litter size. Estrus detection was done at 8:30 a.m. and 5:00 p.m. The WEI was categorized as short (<100 h), medium (100-120 h) and long (>120 h). The ED was categorized as short (<60 h), medium (60-72 h) and long (>72 h). Mean lactation length was 14.6 days, mean WEI was 124.5 h and mean ED was 69 h. In each weaning group, females received either one or two AI, following a breeding schedule based on the estrus profile. In single-mated females, Al was performed 36 h after the beginning of estrus. In double-mated females, the first AI was done 24 h after the beginning of estrus and the second AI occurred 12 h later. The period of estrus detection had no effect (P > 0.05) on WEI, ED, FR, total born (TB) and live born litter size (LB). Mean FR was 82.6%, mean TB was 10.0% and mean LB was 9.2%. Mean ED was shorter (P < 0.03) for females having medium and long WEI (67.0 and 65.4 h, respectively) than for those having short WEI (72.2 h). A linear regression analysis identified a weak (R2 = 0.02) but significant negative association between ED and WEI (P = 0.05). The WEI did not influence FR (P > 0.05). Total litter size for females having short WEI (9.4) was lower (P < 0.03) than for those having long WEI (10.4). Also, LB for females having medium and long WEI (9.7-9.8) was higher (P < 0.05) than for those having short WEI (8.7). AI frequency had no effect on FR (P > 0.05). TB and LB litter size were lower (P < 0.05) for single-mated females (9.6 and 9.0, respectively) than for double-mated females (10.7 and 9.6, respectively). Double Al was associated with higher subsequent litter size. However, breeding schedules based only on estrus profile may not be precise in determining ideal breeding time, since females having short WEI had the longest ED and produced the lowest litter size. 相似文献
163.
Identification of an African swine fever virus gene with similarity to a myeloid differentiation primary response gene and a neurovirulence-associated gene of herpes simplex virus. 下载免费PDF全文
Here we describe an open reading frame (LMW23-NL) in the African swine fever virus genome that possesses striking similarity to a murine myeloid differentiation primary response gene (MyD116) and the neurovirulence-associated gene (ICP34.5) of herpes simplex virus. In all three proteins, a centrally located acidic region precedes a highly conserved, hydrophilic 56-amino-acid domain located at the carboxy terminus. LMW23-NL predicts a highly basic protein of 184 amino acids with an estimated molecular mass of 21.3 kDa. The similarity of LMW23-NL to genes involved in myeloid cell differentiation and viral host range suggests a role for it in African swine fever virus host range. 相似文献
164.
The action of uroporphyrin I (URO I) on the activity of red cell uroporphyrinogen decarboxylase (URO-D) in the dark and under UV light was studied. Light-dependent-and light-independent inactivation was observed. Both effects increased at increasing concentrations of URO I, the former reached its maximum at 150 microM of sensitizer. At 100 microM of URO I, both light and dark inactivation were temperature dependent amounting to about 50% at 30-37 degrees C. The velocity of dark inactivation increased with increasing temperature in the range of 0 to 45 degrees C. Photoinactivation can be ascribed to primary oxidation of essential amino acids, very likely histidyl residues, followed by secondary inter or intrapeptide cross-linking. Dark inactivation could be the result of both oxidation and cross-linking (although to a less degree than that produced by light) and also direct inhibition of the enzyme by induced conformational changes at its active site through binding of the porphyrin to the protein. When the action of URO I was tested on partially purified URO-D, the enzyme appeared to be more susceptible to the dark than to the light effect. 相似文献
165.
On the phospholipids of Bacillus megaterium 总被引:12,自引:0,他引:12
166.
I. Lee‐Montero A. Navarro Y. Borrell M. García‐Celdrán N. Martín D. Negrín‐Báez G. Blanco E. Armero C. Berbel M. J. Zamorano J. J. Sánchez A. Estévez G. Ramis M. Manchado J. M. Afonso 《Animal genetics》2013,44(5):533-546
The high number of multiplex PCRs developed for gilthead seabream (Sparus aurata L.) from many different microsatellite markers does not allow comparison among populations. This highlights the need for developing a reproducible panel of markers, which can be used with safety and reliability by all users. In this study, the first standardised panel of two new microsatellite multiplex PCRs was developed for this species. Primers of 138 specific microsatellites from the genetic linkage map were redesigned and evaluated according to their genetic variability, allele size range and genotyping reliability. A protocol to identify and classify genotyping errors or potential errors was proposed to assess the reliability of each marker. Two new multiplex PCRs from the best assessed markers were designed with 11 markers in each, named SMsa1 and SMsa2 (SuperMultiplex Sparus aurata). Three broodstocks (59, 47 and 98 breeders) from different Spanish companies, and a sample of 80 offspring from each one, were analysed to validate the usefulness of these multiplexes in the parental assignation. It was possible to assign each offspring to a single parent pair (100% success) using the exclusion method with SMsa1 and/or SMsa2. In each genotyped a reference sample (Ref‐sa) was used, and its DNA is available on request similar to the kits of bin set to genotype by genemapper (v.3.7) software (kit‐SMsa1 and kit‐SMsa2). This will be a robust and effective tool for pedigree analysis or characterisation of populations and will be proposed as an international panel for this species. 相似文献
167.
Amanda Ribeiro dos Santos Aline Dionizio Mileni da Silva Fernandes Marília Afonso Rabelo Buzalaf Beatriz Pereira Dbora de Ftima Almeida Donanzam Sergio Marrone Ribeiro Anamaria Mello Miranda Paniago Ricardo de Souza Cavalcante Rinaldo Poncio Mendes James Venturini 《PLoS neglected tropical diseases》2021,15(8)
BackgroundPulmonary sequelae (PS) in patients with chronic paracoccidioidomycosis (PCM) typically include pulmonary fibrosis and emphysema. Knowledge of the molecular pathways involved in PS of PCM is required for treatment and biomarker identification.Methodology/Principal findingsThis non-concurrent cohort study included 29 patients with pulmonary PCM that were followed before and after treatment. From this group, 17 patients evolved to mild/ moderate PS and 12 evolved severe PS. Sera from patients were evaluated before treatment and at clinical cure, serological cure, and apparent cure. A nanoACQUITY UPLC-Xevo QT MS system and PLGS software were used to identify serum differentially expressed proteins, data are available via ProteomeXchange with identifier PXD026906. Serum differentially expressed proteins were then categorized using Cytoscape software and the Reactome pathway database. Seventy-two differentially expressed serum proteins were identified in patients with severe PS compared with patients with mild/moderate PS. Most proteins altered in severe PS were involved in wound healing, inflammatory response, and oxygen transport pathways. Before treatment and at clinical cure, signaling proteins participating in wound healing, complement cascade, cholesterol transport and retinoid metabolism pathways were downregulated in patients with severe PS, whereas signaling proteins in gluconeogenesis and gas exchange pathways were upregulated. At serological cure, the pattern of protein expression reversed. At apparent cure pathways related with tissue repair (fibrosis) became downregulated, and pathway related oxygen transport became upregulated. Additionally, we identified 15 proteins as candidate biomarkers for severe PS.Conclusions/SignificanceDevelopment of severe PS is related to increased expression of proteins involved in glycolytic pathway and oxygen exchange), indicative of the greater cellular activity and replication associated with early dysregulation of wound healing and aberrant tissue repair. Our findings provide new targets to study mechanisms of PS in PCM, as well as potential biomarkers. 相似文献
168.
Prior Exercise Training Prevent Hyperglycemia in STZ Mice by Increasing Hepatic Glycogen and Mitochondrial Function on Skeletal Muscle 下载免费PDF全文
169.
Clemens Küpper Scott V. Edwards András Kosztolányi Monif Alrashidi Terry Burke Philipp Herrmann Araceli Argüelles‐Tico Juan A. Amat Mohamed Amezian Afonso Rocha Hermann Hötker Anton Ivanov Joseph Chernicko Tamás Székely 《Molecular ecology》2012,21(23):5864-5879
Gene flow promotes genetic homogeneity of species in time and space. Gene flow can be modulated by sex‐biased dispersal that links population genetics to mating systems. We investigated the phylogeography of the widely distributed Kentish plover Charadrius alexandrinus. This small shorebird has a large breeding range spanning from Western Europe to Japan and exhibits an unusually flexible mating system with high female breeding dispersal. We analysed genetic structure and gene flow using a 427‐bp fragment of the mitochondrial (mtDNA) control region, 21 autosomal microsatellite markers and a Z microsatellite marker in 397 unrelated individuals from 21 locations. We found no structure or isolation‐by‐distance over the continental range. However, island populations had low genetic diversity and were moderately differentiated from mainland locations. Genetic differentiation based on autosomal markers was positively correlated with distance between mainland and each island. Comparisons of uniparentally and biparentally inherited markers were consistent with female‐biased gene flow. Maternally inherited mtDNA was less structured, whereas the Z‐chromosomal marker was more structured than autosomal microsatellites. Adult males were more related than females within genetic clusters. Taken together, our results suggest a prominent role for polyandrous females in maintaining genetic homogeneity across large geographic distances. 相似文献
170.
Monica Alves Peter Sol Reinach Jayter Silva Paula Antonio Augusto Vellasco e Cruz Leticia Bachette Jacqueline Faustino Francisco Penteado Aranha Afonso Vigorito Carmino Antonio de Souza Eduardo Melani Rocha 《PloS one》2014,9(5)