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Parts L Hedman ÅK Keildson S Knights AJ Abreu-Goodger C van de Bunt M Guerra-Assunção JA Bartonicek N van Dongen S Mägi R Nisbet J Barrett A Rantalainen M Nica AC Quail MA Small KS Glass D Enright AJ Winn J;MuTHER Consortium Deloukas P Dermitzakis ET McCarthy MI Spector TD Durbin R Lindgren CM 《PLoS genetics》2012,8(5):e1002704
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Soares JB Pimentel-Nunes P Afonso L Rolanda C Lopes P Roncon-Albuquerque R Gonçalves N Boal-Carvalho I Pardal F Lopes S Macedo G Lara-Santos L Henrique R Moreira-Dias L Gonçalves R Dinis-Ribeiro M Leite-Moreira AF 《Innate immunity》2012,18(5):700-708
We evaluated expression of TLR2, TLR4 and proinflammatory genes [NF-κB, TNF-α, cyclooxygenase-2 (COX-2)] in liver samples of patients in different stages of liver disease. Fifteen patients with unexplained transaminases elevation (reference group), 22 with viral chronic hepatitis (hepatitis group), 14 with virus-induced severe fibrosis/cirrhosis (cirrhosis group) and 10 with hepatocarcinoma (hepatocarcinoma group) were consecutively included in the study. Quantification of TLR2, TLR4, NF-κB, TNF-α and COX-2 mRNA was done by real-time RT-PCR and TLR2 and TLR4 protein expression was evaluated by immunohistochemistry. Compared with reference, TLR2 and TLR4 mRNA was increased in hepatitis (TLR2: 2.66?±?0.69; TLR4: 3.11?±?0.79; P?0.05) and cirrhosis (TLR2: 2.14?±?0.5; TLR4: 1.74?±?0.27; P?0.05) and decreased in hepatocarcinoma (TLR2: 0.48?±?0.15; TLR4: 0.54?±?0.10; P?0.05). This associated with increased TNF-α and COX-2 mRNA in hepatitis (TNF-α: 3.24?±?0.79; COX-2: 2.47?±?0.36; P?0.05) and cirrhosis (TNF-α: 1.73?±?0.28; COX-2: 1.8?±?0.35, P?0.05), whereas NF-κB mRNA was increased in hepatitis (2.42?±?0.31; P?0.05) and unchanged in cirrhosis (1.34?±?0.17; P?=?0.3). Hepatocarcinoma presented increased COX-2 mRNA (1.63?±?0.15; P?0.05) and maintained (at decreased levels) mRNA of NF-κB (0.52?±?0.12) and TNF-α (0.52?±?0.12; P?0.05, all genes). Immunohistochemistry confirmed increased expression of TLR2 and TLR4 in hepatitis and cirrhosis and maintained expression in hepatocarcinoma. Upregulation of TLR2, TLR4 and their proinflammatory mediators is associated with virus-induced hepatic IFC sequence. 相似文献
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Molecular mapping of soybean rust (Phakopsora pachyrhizi) resistance genes: discovery of a novel locus and alleles 总被引:1,自引:1,他引:0
Garcia A Calvo ES de Souza Kiihl RA Harada A Hiromoto DM Vieira LG 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(4):545-553
Soybean production in South and North America has recently been threatened by the widespread dissemination of soybean rust (SBR) caused by the fungus Phakopsora pachyrhizi. Currently, chemical spray containing fungicides is the only effective method to control the disease. This strategy increases production costs and exposes the environment to higher levels of fungicides. As a first step towards the development of SBR resistant cultivars, we studied the genetic basis of SBR resistance in five F(2) populations derived from crossing the Brazilian-adapted susceptible cultivar CD 208 to each of five different plant introductions (PI 200487, PI 200526, PI 230970, PI 459025, PI 471904) carrying SBR-resistant genes (Rpp). Molecular mapping of SBR-resistance genes was performed in three of these PIs (PI 459025, PI 200526, PI 471904), and also in two other PIs (PI 200456 and 224270). The strategy mapped two genes present in PI 230970 and PI 459025, the original sources of Rpp2 and Rpp4, to linkage groups (LG) J and G, respectively. A new SBR resistance locus, rpp5 was mapped in the LG-N. Together, the genetic and molecular analysis suggested multiple alleles or closely linked genes that govern SBR resistance in soybean. 相似文献
56.
O-acetylation is one of the major modifications of sialic acids that significantly alters biological properties of the parent molecule. These O-acetylated forms are components of the cellular membrane and can affect physiological and pathological responses. Understanding the role of N-glycans in physiology is of increasing relevance to cellular biologists in various disciplines who study glycoproteomics yet lack information regarding the function of the attached glycans. It is well known that stress may decrease immune function in fish; however, there are only few suitable biomarkers available to monitor the physiological responses under the stress conditions. This study is the first report on the effect of stress on the profile of O-acetylation of sialic acids in fish serum. In order to preserve the relevant structural characteristics as much as possible, native N-glycans were directly analyzed using CE-MS. We have characterized the N-glycans in serum of salmon (Salmo salar) exposed to long-term handling stress (15 s out of the water, daily for 4 wk) and compared with the results obtained from sera of control fish. The results indicated that major N-glycans in salmon serum contained mono-acetylated sialic acids (83%), and that the O-acetylation pattern of sialic acids could be altered by long-term stress. 相似文献
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A thermodilution flowmeter 总被引:1,自引:0,他引:1
S Afonso 《Journal of applied physiology》1966,21(6):1883-1886
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