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81.
Sourour Neji Ines Hadrich Amine Ilahi Houaida Trabelsi Hedi Chelly Nadia Mahfoudh Fatma Cheikhrouhou Hayet Sellami Fattouma Makni Ali Ayadi 《Mycopathologia》2018,183(5):765-775
Background
The Candida parapsilosis complex species has emerged as an important cause of human disease. The molecular identification of C. parapsilosis isolates at the species level can be helpful for epidemiological studies and then for the establishment of appropriate therapies and prophylactic measures.Methods
The present study was undertaken to analyze 13 short tandem repeat (STR) markers (7 minisatellites and 6 microsatellites) in a global set of 182 C. parapsilosis complex isolates from different origins including invasive and superficial clinical sites.Results
Upon the analysis of 182 strains of C. parapsilosis complex species, 10–17 haplotypes were detected for each minisatellite marker. The combination of 7 minisatellite markers yielded 121 different genotypes with a 0.995 D value. Upon the analysis of 114 isolates (68 from invasive infections and 46 from superficial infections), 21–32 genotypes were detected for each microsatellite marker. The combination of all 13 markers yielded 96 different genotypes among 114 isolates with a high degree of discrimination (0.997 D value).The same multilocus genotype was shared by isolates recovered from some patients and from the hand of theirs correspondent healthcare worker. For another patient, the same multilocus genotype of C. metapsilosis was detected in blood and skin confirming that candidemia usually arises as an endogenous infection following prior colonization.Conclusions
These STR markers are a valuable tool for the differentiation of C. parapsilosis complex strains, to support epidemiological investigations especially studies of strain relatedness and pathways of transmission.82.
Siala M Jaulhac B Gdoura R Sibilia J Fourati H Younes M Baklouti S Bargaoui N Sellami S Znazen A Barthel C Collin E Hammami A Sghir A 《Arthritis research & therapy》2008,10(2):R40-14
Introduction
Bacteria and/or their antigens have been implicated in the pathogenesis of reactive arthritis (ReA). Several studies have reported the presence of bacterial antigens and nucleic acids of bacteria other than those specified by diagnostic criteria for ReA in joint specimens from patients with ReA and various arthritides. The present study was conducted to detect any bacterial DNA and identify bacterial species that are present in the synovial tissue of Tunisian patients with reactive arthritis and undifferentiated arthritis (UA) using PCR, cloning and sequencing.Methods
We examined synovial tissue samples from 28 patients: six patients with ReA and nine with UA, and a control group consisting of seven patients with rheumatoid arthritis and six with osteoarthritis (OA). Using broad-range bacterial PCR producing a 1,400-base-pair fragment from the 16S rRNA gene, at least 24 clones were sequenced for each synovial tissue sample. To identify the corresponding bacteria, DNA sequences were compared with sequences from the EMBL (European Molecular Biology Laboratory) database.Results
Bacterial DNA was detected in 75% of the 28 synovial tissue samples. DNA from 68 various bacterial species were found in ReA and UA samples, whereas DNA from 12 bacteria were detected in control group samples. Most of the bacterial DNAs detected were from skin or intestinal bacteria. DNA from bacteria known to trigger ReA, such as Shigella flexneri and Shigella sonnei, were detected in ReA and UA samples of synovial tissue and not in control samples. DNA from various bacterial species detected in this study have not previously been found in synovial samples.Conclusion
This study is the first to use broad-range PCR targeting the full 16S rRNA gene for detection of bacterial DNA in synovial tissue. We detected DNA from a wide spectrum of bacterial species, including those known to be involved in ReA and others not previously associated with ReA or related arthritis. The pathogenic significance of some of these intrasynovial bacterial DNAs remains unclear. 相似文献83.
Sellami R Gharbi F Rejeb S Rejeb MN Henchi B Echevarria G Morel JL 《International journal of phytoremediation》2012,14(6):609-620
A pot experiment was conducted to investigate the effect of nickel concentration on physiological characteristics of Alyssum murale when grown in a soil mixed with sewage sludge (at the rate of 2.8%). Two types of sludge were used: agricultural sewage sludge (S1) and industrial sewage sludge with an increasing nickel concentration (S2, S3, and S4). Results showed that Ni in shoots was higher than Ni in roots. A. murale is able to concentrate up to 12730 mg/kg Ni in leaves. The highest dry matter yield was observed with plants grown with agricultural sewage sludge. An addition of S2 and S3 increased shoot biomass. However, application of S4 reduced 40% shoot dry weight as compared to the control Ni treatment did not affect all chlorophyll fluorescence parameters. The F(v)/F(m) ratio was stable between Ni treatments. Photosynthesis rate (A) increased with agricultural sewage sludge, but remained stable with variable Ni rates from the industrial sludge. The chlorophyll content increased with S1, S2 and S3 but it remains constant with S4 when compared to the control Therefore, high nickel concentration did not affect the function of the photosynthetic machine of A. murale. 相似文献
84.
Ibrahim Aissa Vijaykumar D. Nimbarte Afifa Zardi‐Bergaoui Mansour Znati Guido Flamini Roberta Ascrizzi Hichem Ben Jannet 《化学与生物多样性》2019,16(4)
The chemical composition of the essential oil (LEO) and its volatile fractions (V1–V10) collected during the hydrodistillation process every 15 min from the fresh leaves of I. viscosa (L.), growing in Tunisia, were analyzed by GC‐FID and GC/MS. Eighty‐two compounds, representing 90.9–99.4 % of the total samples, were identified. The crude essential oil (LEO) and its fractions (V1–V10) were characterized by the presence of a high amount of oxygenated sesquiterpenes (82.7–95.8 %). Isocostic acid ( 1 ) was found to be the most abundant component (37.4–83.9 %) and was isolated from the same essential oil over silica gel column chromatography and identified by spectroscopic methods (1H, 13C, DEPT 135 NMR and EI‐MS) and by comparison with literature data. Furthermore, the fresh leaves essential oil (LEO), its volatile fractions (V1–V10) as well as compound 1 were screened for their antibacterial, antityrosinase, anticholinesterase and anti‐5‐lipoxygenase activities. It was found that the isolated compound 1 exhibited an interesting antibacterial activity against Staphylococcus aureus ATCC 25923 (MIC=32 μg/mL) and Enterococcus faecalis ATCC 29212 (MIC=32 μg/mL) and the highest antityrosinase activity (IC50=13.82±0.87 μg/mL). Compound 1 was also found to be able to strongly inhibit 5‐lipoxygenase with an IC50 value of 59.21±0.85 μg/mL. The bioactivity and drug likeness scores of compound 1 were calculated using Molinspiration software and interpreted, and the structure‐activity relationship (SAR) was discussed with the help of molecular docking analysis. 相似文献
85.
Jiangtian Chen Wencke Reiher Christiane Hermann-Luibl Azza Sellami Paola Cognigni Shu Kondo Charlotte Helfrich-F?rster Jan A. Veenstra Christian Wegener 《PLoS genetics》2016,12(9)
Feeding and sleep are fundamental behaviours with significant interconnections and cross-modulations. The circadian system and peptidergic signals are important components of this modulation, but still little is known about the mechanisms and networks by which they interact to regulate feeding and sleep. We show that specific thermogenetic activation of peptidergic Allatostatin A (AstA)-expressing PLP neurons and enteroendocrine cells reduces feeding and promotes sleep in the fruit fly Drosophila. The effects of AstA cell activation are mediated by AstA peptides with receptors homolog to galanin receptors subserving similar and apparently conserved functions in vertebrates. We further identify the PLP neurons as a downstream target of the neuropeptide pigment-dispersing factor (PDF), an output factor of the circadian clock. PLP neurons are contacted by PDF-expressing clock neurons, and express a functional PDF receptor demonstrated by cAMP imaging. Silencing of AstA signalling and continuous input to AstA cells by tethered PDF changes the sleep/activity ratio in opposite directions but does not affect rhythmicity. Taken together, our results suggest that pleiotropic AstA signalling by a distinct neuronal and enteroendocrine AstA cell subset adapts the fly to a digestive energy-saving state which can be modulated by PDF. 相似文献
86.
S.?Neji F.?Makni F.?Cheikhrouhou H.?Sellami S.?Boudaya H.?Turki A.?AyadiEmail author 《Mycopathologia》2010,170(5):353-355
This is a first case of Trichophyton soudanense isolated from Ivoiran student in Tunisia. A 24-year-old man was addressed for extensive erythematous, scaling lesions. Examination
disclosed tinea capitis, tinea corporis, tinea pedis, and onychomycosis of toenails and fingernails. Isolates were identified
as Trichophyton soudanense on the basis of macroscopy and microscopy colony characteristics. The patient was treated with fluconazole, topical econazole,
and ciclopiroxolamine varnish. Although T. soudanense was identified since the late 1950s outside the African continent especially in the North America, Brazil, Australia, and
many European countries, this is the first case reported in Tunisia. Accessibility to our universities for African students
makes possible the emergence of this dermatophyte. 相似文献
87.
S. Neji F. Makni H. Sellami F. Cheikhrouhou A. Sellami Ali Ayadi 《Mycopathologia》2009,167(6):351-353
This is the first case of Microsporum ferrugineum isolated from a Tunisian patient. A 60-year-old man was admitted for tinea sycosis associated with circinate herpes of the
hand. Examination disclosed diffuse erythematic and perifollicular papules and pustules in the beard area. Typical ringworm
vesiculo-pustular lesions involved skin of the hand. Isolates were identified as Microsporum sp on the basis of macroscopic and microscopic colony characteristics. The diagnosis of M. ferrugineum was confirmed by PCR sequencing of Chitin Synthase1 gene. The patient was treated successfully with Griseofulvin, which was
administered for 4 weeks. 相似文献
88.
M Sellami M Gamoudi K Krichen A Kharrat K Ben Romdhane M Maalej 《Archives de l'Institut Pasteur de Tunis》1991,68(1-2):33-41
Activation of cellular proto-oncogenes can be detected in several human cancers. In the current study, alterations of C-erb B2 gene in primary human breast cancers were investigated. It was found to be amplified from 2 to 30 fold in 25% of the tumors. Correlations of gene amplification with several disease parameters was evaluated. 相似文献
89.
Cluster Computing - Estimating software enhancement efforts became a challenging task in software project management. Recent researches focused on identifying the best machine learning algorithms... 相似文献
90.
Ghorbel Koubaa Fatma Chaâbane Mariem Chiab Nour Jdidi Hajer Sefi Mediha Boudawara Ons Turki Mouna Gargouri Bouzid Radhia Boudawara Sellami Tahia Makni Ayadi Fatma El Feki Abdelfattah 《Biometals》2022,35(5):833-851
BioMetals - Vanadium has been shown to catalyze the generation of reactive oxygen species. Since free radical production and lipid peroxidation are potentially important mediators in testicular... 相似文献