全文获取类型
收费全文 | 4841篇 |
免费 | 355篇 |
国内免费 | 2篇 |
出版年
2023年 | 28篇 |
2022年 | 54篇 |
2021年 | 131篇 |
2020年 | 86篇 |
2019年 | 104篇 |
2018年 | 147篇 |
2017年 | 120篇 |
2016年 | 170篇 |
2015年 | 270篇 |
2014年 | 286篇 |
2013年 | 363篇 |
2012年 | 400篇 |
2011年 | 410篇 |
2010年 | 208篇 |
2009年 | 219篇 |
2008年 | 241篇 |
2007年 | 251篇 |
2006年 | 229篇 |
2005年 | 242篇 |
2004年 | 205篇 |
2003年 | 195篇 |
2002年 | 184篇 |
2001年 | 58篇 |
2000年 | 49篇 |
1999年 | 57篇 |
1998年 | 39篇 |
1997年 | 35篇 |
1996年 | 28篇 |
1995年 | 28篇 |
1994年 | 24篇 |
1993年 | 25篇 |
1992年 | 23篇 |
1991年 | 26篇 |
1990年 | 22篇 |
1989年 | 26篇 |
1988年 | 20篇 |
1987年 | 16篇 |
1986年 | 8篇 |
1985年 | 16篇 |
1984年 | 12篇 |
1983年 | 11篇 |
1982年 | 14篇 |
1981年 | 7篇 |
1980年 | 14篇 |
1979年 | 7篇 |
1978年 | 12篇 |
1977年 | 8篇 |
1975年 | 9篇 |
1973年 | 15篇 |
1970年 | 6篇 |
排序方式: 共有5198条查询结果,搜索用时 15 毫秒
41.
42.
While investigating dinoflagellate cyst assemblages in surface sediments of the Gulfs of Naples and Salerno (Mediterranean Sea), we found a new calcareous resting cyst. This cyst has a round to oval body surrounded by a thick mineral layer, which gives it the shape of a Napoleon hat, with a flat, oval face bearing the archeopyle and a convex keel on the opposite side. The cyst shape is variable in both natural samples and clonal cultures. The organic membrane underlying the calcareous covering is resistant to acetolysis, thus demonstrating the presence of sporopolleninlike material. The cyst germinated into a motile stage having the same morphological features and thecal plate pattern as Peridinium tyrrhenicum Balech. We believe the validity of the genus Pentapharsodinium Indelicato & Loeblich should be accepted. Based on the comparative examination of the species we collected and of a similar species, Pentapharsodinium trachodium Indelicato & Loeblich, we propose Pentapharsodinium tyrrhenicum as a new combination for Peridinium tyrrhenicum. The genus Pentapharsodinium also includes P. dalei Indelicato & Loeblich (= Peridinium faeroense Dale), which produces spiny, organic-walled cysts. The presence of species forming calcareous cysts and species producing noncalcareous cysts in the same genus raises questions about maintaining the family Calciodinellaceae. This family should only include calcareous cyst-forming peridinioids, in order to maintain a unified system of classification of fossil and recent dinoflagellates. 相似文献
43.
Purification and characterization of two 1,4-beta-xylan endohydrolases from the rumen fungus Neocallimastix frontalis. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Two beta-endoxylanases produced by Neocallimastix frontalis have been purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography. Xylanase I is a nonglycosylated protein with an apparent molecular mass of 45 kDa. Xylanase II is a glycoprotein with an apparent molecular mass of 70 kDa. The pH optima of these enzymes were 5.5 and 6, respectively, and the temperature optimum was 55 degrees C for each enzyme. The endo mode of action of the enzymes was revealed by thin-layer chromatography of xylan hydrolysates. Antibodies raised against each purified protein exhibited no cross-reaction, confirming the biochemical specificities of the enzymes. Both enzymes exhibited carboxymethyl cellulase activity, and xylanase I was absorbed on crystalline cellulose, indicating that these enzymes might belong to the F family of beta-1,4-glycanases. 相似文献
44.
C. Gutierrez G. Garcia-Casado R. Snchez-Monge L. Gomez P. Castaera G. Salcedo 《Entomologia Experimentalis et Applicata》1993,66(1):47-52
Crude α-amylase preparations from seven Lepidoptera pests were susceptible to inhibition by salt-soluble proteins of bread
wheat (Triticum aestivum L.) endosperm. Protein fractions that corresponded to tetrameric, dimeric, and monomeric wheat α-amylase inhibitors, were
decreasingly effective against the insect α-amylase activity. To further confirm these results, purified inhibitors were tested
against an α-amylase preparation fromEphestia kuehniella (Zeller). This preparation showed decreased activity when increasing amounts of an heterotetrameric inhibitor (reconstituted
from its isolated subunits WTAI-CM2, -CM3 and -CM16) were assayed. Activity was only partially inhibited by homodimeric (WDAI-1,
synonym 0.53; WDAI-2, synonym 0.19) and monomeric (WMAI-1, synonym 0.28) inhibitors. 相似文献
45.
Adriana García 《Hydrobiologia》1993,267(1-3):143-154
Only recently have extant Lamprothamnium species been reported from the American continent. L. succinctum (A. Br. in Asch.) R. D. W. was found in Lago Titicaca, Bolivia and L. haesseliae Dont. en Laguna Luro and Laguna La Salada, Argentina.Fossil gyrogonites of L. succinctum and L. haesseliae, however, are here reported to be widely distributed in Quaternary sediments of Argentina, associated with other charophytes, ostracods and foraminiferans. Localities include Laguna del Siasgo, Laguna Salada Grande, Laguna Mar Chiquita, Laguna La Amarga and Salina del Bebedero.
Lamprothamnium is a genus that prefers shallow, alkaline, hyposaline to mesosaline environments. Analysis of the characteristics of Laguna La Salada contributes information on the factors that influence the distribution of the genus.The morphology characteristics of the gyrogonites and oospores of L. haesseliae and the gyrogonites of L. succinctum are described. The use of Lamprothamnium as a biomarker and its application in the reconstruction of Quaternary saline environments are also discussed. 相似文献
46.
Gustavo A. Londoño Juan Pablo Gomez Manuel A. Sánchez-Martínez Douglas J. Levey Scott K. Robinson 《Ecology letters》2023,26(4):609-620
Tropical montane communities host the world's highest beta diversity of birds, a phenomenon usually attributed to community turnover caused by changes in biotic and abiotic factors along elevation gradients. Yet, empirical data on most biotic factors are lacking. Nest predation is thought to be especially important because it appears to be common and can change selective pressures underlying life history traits, which can alter competitive interactions. We monitored 2538 nests, 338 of which had known nest predators, to evaluate if nest predation changes along a tropical elevational gradient. We found that nest predation decreased with elevation, reflecting the loss of lowland predators that do not tolerate colder climates. We found different “super” nest predators at each elevation that accounted for a high percentage of events, suggesting that selection pressures exerted by nest predator communities may be less diffuse than has been hypothesized, at least for birds nesting in the understory. 相似文献
47.
Daniel E. Gomez Jacqueline L. Hartzler Robert H. Corbitt Alexander M. Nason Unnur P. Thorgeirsson 《In vitro cellular & developmental biology. Animal》1993,29(6):451-455
Summary We describe a fast and reproducible method that can be used as a final step in obtaining pure populations of liver endothelial
cells. This method employs endothelial cell specific lectin covalently bound to magnetic polystyrene beads (Dynabeads). Evonymus
europaeus agglutinin (EEA)-coated Dynabeads were used to purify monkey liver endothelium from Percoll gradient separated nonparenchymal
cells. EEA-coated beads were also successfully used to purify monkey aortic endothelial cells. The endothelial cells grew
to confluence as a cobblestonelike monolayer, expressed Factor VIII related antigen, and incorporated acetylated-low density
lipoprotein. The magnetic beads seemed not to modify the normal properties of the isolated endothelium, thus facilitating
their use in experimental studies. This immunomagnetic separation technique may be applicable for purification of endothelial
cells from a wide variety of tissue sources. 相似文献
48.
49.
Rafael Radi Stephen Sims Adriana Cassina Julio F. Turrens 《Free radical biology & medicine》1993,15(6)
A recent report (Radi et al., J. Biol. Chem. 266:22028–22034, 1991) showed that rat heart mitochondria contain catalase. The protective role of mitochondrial catalase was tested by exposing heart or kidney mitochondria and mitoplasts to two oxidants (H2O2) or tert-butyl hydroperoxide, t-BOOH), estimating lipid peroxidation (as thiobarbituric acid-reactive substances, TBARS) and overall oxidative stress (as chemiluminescence). Additional controls included heart and kidney preparations from aminotriazole-treated (catalase-depleted) rats. Both oxidants increased TBARS in catalase-free preparations to similar extents over their respective controls (between 200 to 350%). In catalase-containing preparations, H2O2 lipid peroxidation increased by only 40 to 96% over controls. Similar qualitative results were obtained when measuring chemiluminescence. The catalytic role of cytochrome c in mitochondrial lipid peroxidation was investigated by exposing either control or cytochrome-c-depleted kidney mitoplasts (catalase free) to either H2O2 or t-BOOH. Hydrogen-peroxide-dependent mitochondrial lipid peroxidation varied with cytochrome c concentrations, remaining close to controls when cytochrome c concentration decreased by 66%, even though there was no catalase present. Tert-butyl hydroperoxide-dependent lipid peroxidation was less affected by cytochrome c remaining 2.3-fold above controls under the same conditions, suggesting that organic peroxides are more likely to remain in the less polar membrane environment being decomposed by heme or nonheme iron imbedded in the inner mitochondrial membrane. Chemiluminescence was less affected by cytochrome c depletion. Comparing control and cytochrome-c-deficient mitochondria, chemiluminescence was 1.7-fold and 2.8-fold higher when control preparations were challenged with t-BOOH or H2O2, respectively. 相似文献
50.
Diana Villegas-Coronado Jesús Adriana Soto-Guzman Juan Manuel Martínez-Soto Nayelli Guadalupe Teran-Saavedra Ana Maria Guzman-Partida Luz Vazquez-Moreno Ana Gloria Villalba-Villalba Amir Maldonado Irlanda Lagarda-Diaz 《化学与生物多样性》2023,20(7):e202300051
Acute monocytic leukemia is a type of myeloid leukemia that develops in monocytes. The current clinical therapies for leukemia are unsatisfactory due to their side effects and nonspecificity toward target cells. Some lectins display antitumor activity and may specifically recognize cancer cells by binding to carbohydrate structures on their surface. Therefore, this study evaluated the response of the human monocytic leukemia cell lines THP-1 to the Olneya tesota PF2 lectin. The induction of apoptosis and reactive oxygen species production in PF2-treated cells was evaluated by flow cytometry, and the lectin-THP-1 cell interaction and mitochondrial membrane potential were evaluated by confocal fluorescence microscopy. PF2 genotoxicity was evaluated by DNA fragmentation analysis via gel electrophoresis. The results showed that PF2 binds to THP-1 cells, triggers apoptosis and DNA degradation, changes the mitochondrial membrane potential, and increases reactive oxygen species levels in PF2-treated THP-1 cells. These results suggest the potential use of PF2 for developing alternative anticancer treatments with enhanced specificity. 相似文献