Biocolloid retention in partially saturated soils

Unsaturated soils are considered excellent filters for preventing the transport of pathogenic biocolloids to groundwater, but little is known about the actual mechanisms of biocolloid retention. To obtain a better understanding of these processes, a number of visualization experiments were performed and analyzed.     

Cloning of human lysozyme gene and expression in the yeast Saccharomyces cerevisiae

cDNA clones encoding human lysozyme were isolated from a human histiocytic cell line (U-937) and a human placenta cDNA library. The clones, ranging in size from 0.5 to 0.75 kb, were identified by direct hybridization with synthetic oligodeoxynucleotides. The nucleotide sequence coding for the entire protein was determined. The derived amino acid sequence has 100% homology with the published amino acid (aa) sequence; the leader sequence codes for 18 aa. Expression and secretion of human lysozyme in Saccharomyces cerevisiae was achieved by placing the cloned cDNA under the control of a yeast gene promoter (ADH1) and the alpha-factor peptide leader sequence.     

Hauptgedanken des holismus

Résumé La question formulée est la suivante: il s' agit de prouver si la réalité est une unité, une pluralité ou une totalité. Nous ne nous proposons pas trouver des résultats définitifs; nous nous bornerons plutôt à renseigner un nouveau ideal de la connaissance des choses, pour ainsi servir les buts de la science avenir.Sous la prévalence de l'orientation physicale la science tendait à comprendre la nature comme une seule unité. La philosophie qui corresponde à cette conception est le Monisme, qui embrasse tous les systèmes philosophiques fondés sur l'idée mécanistique du monisme primitif deHaeckel jusqu'au positivisme sublime. Toutes ces conceptions ont échoué, car ils n'étaient pas capables d'éliminer les contingences de la réalité.Toute la conception de la nature soutenable du point de vue empirique, doit reconnaître ces contingences de la réalité. Sur cette base on peut comprendre la réalité ou comme une pluralité ou comme une totalité. Selon la doctrine de pluralisme la contingence des lois de la nature (Boutroux) est un phénomène, qu'on doit accepter comme tel. Il donnerait la solution définitive des problèmes philosophiques, à moins qu'il n'existât une autre philosophie de la réalité fondée, elle aussi, sur la contingence de la nature.Cette nouvelle philosophie est le Holisme.À la difference du pluralisme les rayons de la réalité ne constituent pas des domaines absolument autonomes, mais ils forment une totalité organique comparable à celle des membres d'un corps vivant. Dans un corps vivant chaque organe, entre la vie autonome, remplit une fonction spécifique dans l'ensemble total de l'organisme. De la même façon les différents rayons de la réalité ne sont que des organes fonctionnellement différenciés de la nature totale. Par conséquent, la nature n'est pas une unité passive et mécanistique, mais une totalité active et créatrice. Chaque cellule est un membre du tissu, chaque tissu un membre de l'organe et chaque organe un membre de l'organisme total; cependant chacun d'eux a sa propre existence. D'une manière analogue la réalité physique forme un membre de la réalité organique, la réalité organique un membre de la réalité psychique, la réalité psychique un membre de la réalité sociale, et enfin la réalité sociale un membre de la réalité historique. Toute fois ces rayons conservent leur autonomie et leur contingence. Or, les membres d'un organisme ne peuvent être expliqués qu'en partant de l'organe supérieur, c'est à dire pour comprendre les fonctions de la cellule il faut d'abord connaître celle du tissu et ainsi de suite etc. Les différents rayons de la réalité ne peuvent donc être compris qu'en dérivant, en chaque cas, la réalité moins compliquée, ce qui signifie par exemple qu'on doit dériver la réalité physique de la réalité organique, la réalité organique de la réalité psychique etc. Les détails de l'argumentation sont donnés dans le traité lui-même.En résumé il résulte une conception biologique de la réalité totale au lieu de la conception physique qui prévalait jusqu'ici.

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Summary The question dealt with in the article is the following: Is reality a Unity, a Plurality or a Whole. We do not expect to get definit results, we are only interested in pointing out a new ideal of scientific research.Under the predominance of physical thinking science was inclined upon nature as a Unity. The philosophy corresponding with this conception is Monism, to which belong all philosophical systems founded on the mecanistic idea from the primitive Monism ofHaeckel to the most sophisticated Positivism. All these conceptions had to be rejected, because they were not able to eliminate the contingences of reality.Every conception of nature consistent with experience must recognize these contingences of reality. On this bases one can take reality either as a plurality or as a whole. According to the doctrine of pluralism contingence of the laws of nature (Boutroux) is an original phenomenon which has to be accepted as such. This would mean the definit solution of the philosophical problems, if this would prove to be the only philosophy founded on the principle of contingence.But there is still such an another conception that is Holism. Here contrary to Pluralism, the domains of reality are not absolutely autonomous but form an organic whole comparable to that of the organs of a living body. In a living body each organ besides having its autonomous life, fulfils a special function in the whole of the organism. In the same science the different domains of reality are only the functionally differenciated organs of the whole nature. Consequently nature is not a passive and mecanistic unity, but an active and creative whole. Each cell is a member of a tissue, each tissue a member of an organ and the organ a member of the whole organism; each of them however has its personal and relatively autonomous existence. In the same way physical reality forms a member of organical reality, organical reality a member of psiquical reality, psiquical reality a member of social reality and social reality a member of historical reality. These domains also keep their autonomy and contingence. So the members of an organism can only be explained starting from the superior organ, i.e. in order to understand the functions of the cell one must first know those of the tissue etc. Though different domains of reality can only be understood by deriving in each case the less complicated reality from the more complicated, for instance the physical reality must be derived from organical reality, organical reality from psiquical reality etc. ... The details of the argumentation are given in the treatise itself. In summary the result is a biological conception of nature as a whole instead of physical conception prevalent up to now.

     

Effects of Prey Presence and Scale on Bobcat Resource Selection during Winter

Factors relevant to resource selection in carnivores may vary across spatial and temporal scales, both in magnitude and rank. Understanding relationships among carnivore occupancy, prey presence, and habitat characteristics, as well as their interactions across multiple scales, is necessary to improve our understanding of resource selection and predict population changes. We used a multi-scale dynamic hierarchical co-occurrence model with camera data to study bobcat and snowshoe hare occupancy in the Upper Peninsula of Michigan during winter 2012–2013. Bobcat presence was influenced at the local scale by snowshoe hare presence, and by road density at the local and larger scale when hare were absent. Hare distribution was related primarily to vegetation cover types, and detectability varied in space and time. Bobcat occupancy dynamics were influenced by different factors depending on the spatial scale considered and the resource availability context. Moreover, considering observed co-occurrence, we suggest that bobcat presence had a greater effect on hare occupancy than hare presence on bobcat occupancy. Our results highlight the importance of studying carnivore distributions in the context of predator-prey relationships and its interactions with environmental covariates at multiple spatial scales. Our approach can be applied to other carnivore species to provide insights beneficial for management and conservation.     

Highly protective alkalinization by ammonia vapor diffusion in viscosimetric DNA damage assessment

A method for the measurement of viscosities correlated to DNA alterations in alkaline homogenate suspensions is described. The alkaline pH shift to afford cell lysis, DNA unfolding, and denaturation is attained by gaseous ammonia diffusion, thus avoiding shear stress from mechanical mixing. At the same time a stabilizing density gradient is established. This solution is run through a plastic measuring tube that is wide enough to minimize the influence of uneven swelling of the lysing DNA-containing components. Flow times under a carefully controlled water head are registered, and their ratios to control solutions are evaluated. The relative viscosities show a strong and irreversible dependence on shear and on DNase treatment and therefore are considered as essentially DNA derived. The time dependence of the lysate viscosities with and without the DNA-damaging agent bleomycin is given and the dose:activity curves of this agent with sponge homogenates from two orders of Porifera are given with their 50% effective concentration values. The dose:activity curve of an extract from a polluted marine point source is demonstrated. The concentration changes in sponges exposed at differently polluted marine sites are shown. The idea of alkalinization through gaseous diffusion in conjunction with a simple measuring device has already proven a sensitive, reliable, and specific tool in the assessment of DNA damage produced under both laboratory and field conditions.