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61.
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Kin recognition, not competitive interactions, predicts root allocation in young Cakile edentula seedling pairs 总被引:1,自引:0,他引:1
? Recent studies have demonstrated sibling vs stranger differences in group root allocation in plants, suggesting that plants have the potential for kin discrimination in competition. However, morphology differences could potentially be generated by competition-based mechanisms. Here, we tested these hypotheses for the sibling vs stranger differences in root allocation in Cakile edentula. ? Seeds were planted in pairs of either kin (siblings) or strangers, from all combinations of eight families, to give eight kin (sibling) and 28 stranger pair identities. Because the species has a seed dimorphism, the 10 replicates of each pair identity included both seed types. Root allocation, size inequality between seedlings in a pair, and competitive ability were derived from measures of biomass and height. ? Cakile edentula seedlings demonstrated the same kin recognition response previously observed in juvenile plants, with lower root allocation in kin pairs than stranger pairs. The seed dimorphism was not associated with root allocation. ? The two competitive mechanisms, genetic differences in competitive ability and increased size inequality in stranger groups, did not explain the root allocation differences in these seedlings. Kin recognition offered the most probable explanation for the differences in root allocation between sibling and stranger pairs. 相似文献
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Adenylate kinase 3 sensitizes cells to cigarette smoke condensate vapor induced cisplatin resistance
Background
The major established etiologic risk factor for bladder cancer is cigarette smoking and one of the major antineoplastic agents used for the treatment of advanced bladder cancer is cisplatin. A number of reports have suggested that cancer patients who smoke while receiving treatment have lower rates of response and decreased efficacy of cancer therapies.Methodology/Principal Findings
In this study, we investigated the effect of cigarette smoke condensate (CSC) vapor on cisplatin toxicity in urothelial cell lines SV-HUC-1 and SCaBER cells. We showed that chronic exposure to CSC vapor induced cisplatin resistance in both cell lines. In addition, we found that the expression of mitochondrial-resident protein adenylate kinase-3 (AK3) is decreased by CSC vapor. We further observed that chronic CSC vapor-exposed cells displayed decreased cellular sensitivity to cisplatin, decreased mitochondrial membrane potential (ΔΨm) and increased basal cellular ROS levels compared to unexposed cells. Re-expression of AK3 in CSC vapor-exposed cells restored cellular sensitivity to cisplatin. Finally, CSC vapor increased the growth of the tumors and also curtail the response of tumor cells to cisplatin chemotherapy in vivo.Conclusions/Significance
The current study provides evidence that chronic CSC vapor exposure affects AK3 expression and renders the cells resistant to cisplatin. 相似文献66.
67.
The unfolded protein response (UPR) activates Ire1, an endoplasmic reticulum (ER) resident transmembrane kinase and ribonuclease (RNase), in response to ER stress. We used an in vivo assay, in which disappearance of the UPR-induced spliced HAC1 messenger ribonucleic acid (mRNA) correlates with the recovery of the ER protein-folding capacity, to investigate the attenuation of the UPR in yeast. We find that, once activated, spliced HAC1 mRNA is sustained in cells expressing Ire1 carrying phosphomimetic mutations within the kinase activation loop, suggesting that dephosphorylation of Ire1 is an important step in RNase deactivation. Additionally, spliced HAC1 mRNA is also sustained after UPR induction in cells expressing Ire1 with mutations in the conserved DFG kinase motif (D828A) or a conserved residue (F842) within the activation loop. The importance of proper Ire1 RNase attenuation is demonstrated by the inability of cells expressing Ire1-D828A to grow under ER stress. We propose that the activity of the Ire1 kinase domain plays a role in attenuating its RNase activity when ER function is recovered. 相似文献
68.
Krishna Baksi Zhenbo Zhang Aditi Banerjee Dipak K. Banerjee 《Glycoconjugate journal》2009,26(6):635-645
Mannosylphospho dolichol synthase (DPMS) is a critical enzyme in the biosynthesis of lipid-linked oligosaccharide (LLO; Glc3Man9GlcNAc2-PP-Dol), a pre-requisite for asparagine-linked (N-linked) protein glycosylation. We have shown earlier that DPMS is important
for angiogenesis, i.e., endothelial cell proliferation. This is true when cAMP is used for intracellular signaling. During cAMP signaling, DPMS
is activated and ER stress is reduced. To understand the activation of DPMS at the molecular level we have isolated a cDNA
clone for the DPMS gene (bDPMS) from the capillary endothelial cells of bovine adrenal medulla. DNA sequencing and the deduced
amino acid sequence have established that bDPMS has a motif to be phosphorylated by cAMP-dependent protein kinase (PKA). Based
on the sequence information Serine 165 has been found to be the phosphorylation target in bDPMS. Hydropathy Index when plotted
against amino acid number indicates the presence of a hydrophobic region around the amino acid residues 120–160, supporting
that bDPMS has one membrane spanning region. The recombinant bDPMS has now been purified as His-tag protein with an apparent
molecular weight of M
r 33 kDa. Additionally, we show here that overexpression of DPMS is indeed angiogenic. The capillary endothelial cells proliferate
at a higher rate carrying the DPMS overexpression plasmid over the parental cells or the vector. 相似文献
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Tapan K. Chaudhuri Vikash K. Verma Aditi Maheshwari 《Progress in biophysics and molecular biology》2009,99(1):42-50
Escherichia coli chaperonins GroEL and GroES are indispensable for survival and growth of the cell since they provide essential assistance to the folding of many newly translated proteins in the cell. Recent studies indicate that a substantial portion of the proteins involved in the host pathways are completely dependent on GroEL–GroES for their folding and hence providing some explanation for why GroEL is essential for cell growth. Many proteins either small-single domain or large multidomains require assistance from GroEL–ES during their lifetime. Proteins of size up to 70 kDa can fold via the cis mechanism during GroEL–ES assisted pathway, but other proteins (>70 kDa) that cannot be pushed inside the cavity of GroEL–ATP complex upon binding of GroES fold by an evolved mechanism called trans. In recent years, much work has been done on revealing facts about the cis mechanism involving the GroEL assisted folding of small proteins whereas the trans mechanism with larger polypeptide substrates still remains under cover. In order to disentangle the role of chaperonin GroEL–GroES in the folding of large E. coli proteins, this review discusses a number of issues like the range of large polypeptide substrates acted on by GroEL. Do all these substrates need the complete chaperonin system along with ATP for their folding? Does GroEL act as foldase or holdase during the process? We conclude with a discussion of the various queries that need to be resolved in the future for an extensive understanding of the mechanism of GroEL mediated folding of large substrate proteins in E. coli cytosol. 相似文献