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171.
Luis Toronjo-Urquiza Adelina E. Acosta-Martin David C. James Tibor Nagy Robert J. Falconer 《Biotechnology progress》2020,36(4):e2980
Catechin compounds have potential benefits for recombinant monoclonal antibody (Mab) production as chemical additives in cell culture media. In this study, four catechin compounds catechin (Cat), epicatechin (EC), gallocatechin-gallate (GCG), and epigallocatechin-gallate (EGCG) were added to cell culture media (at 50 μM) and their effects on the recombinant Chinese hamster ovary (CHO) cell culture, specific productivity, and Mab quality were assessed. The results indicate that the improvement of specific productivity was linked to cell growth inhibition. All catechins caused cell phase growth arrest by lowering the number of cells in the G1/G0 phase and increasing the cells in the S and G2/M phases. Late addition of the catechin resulted in a significantly higher final IgG concentration. Cat and EC caused an improvement in the final antibody titer of 1.5 ± 0.1 and 1.3 ± 0.1 fold, respectively. Catechins with a galloyl group (GCG and EGCG) arrested cell growth and reduced cell specific productivity at the concentrations tested. The Cat-treated IgG was found to have reduced acidic species with a corresponding increase in the main peak. 相似文献
172.
Marta?G. Lete Jesús Sot Hasna Ahyayauch Noelia Fernández-Rivero Adelina Prado Félix?M. Go?i Alicia Alonso 《Biophysical journal》2014,106(5):1092-1100
Recent discoveries on the presence and location of phosphoinositides in the eukaryotic cell nucleoplasm and nuclear membrane prompted us to study the putative interaction of chromatin components with these lipids in model membranes (liposomes). Turbidimetric studies revealed that a variety of histones and histone combinations (H1, H2AH2B, H3H4, octamers) caused a dose-dependent aggregation of phosphatidylcholine vesicles (large unilamellar vesicle or small unilamellar vesicle) containing negatively charged phospholipids. 5 mol % phosphatidylinositol-4-phosphate (PIP) was enough to cause extensive aggregation under our conditions, whereas with phosphatidylinositol (PI) at least 20 mol % was necessary to obtain a similar effect. Histone binding to giant unilamellar vesicle and vesicle aggregation was visualized by confocal microscopy. Histone did not cause vesicle aggregation in the presence of DNA, and the latter was able to disassemble the histone-vesicle aggregates. At DNA/H1 weight ratios 0.1–0.5 DNA- and PIP-bound H1 appear to coexist. Isothermal calorimetry studies revealed that the PIP-H1 association constant was one order of magnitude higher than that of PI-H1, and the corresponding lipid/histone stoichiometries were ∼0.5 and ∼1, respectively. The results suggest that, in the nucleoplasm, a complex interplay of histones, DNA, and phosphoinositides may be taking place, particularly at the nucleoplasmic reticula that reach deep within the nucleoplasm, or during somatic and nonsomatic nuclear envelope assembly. The data described here provide a minimal model for analyzing and understanding the mechanism of these interactions. 相似文献
173.
In the cell, the majority of proteins exist in complexes. Most of these complexes have a constant stoichiometry and thus can be used as internal standards. In this rapid communication, we show that it is possible to calculate a correlation coefficient that reflects the reproducibility of the analytical approach used. The abundance of one subunit in a heterodimer is plotted against the abundance of the other, and this is repeated for all subunits in all heteromers found in the data set. The correlation coefficient obtained (the “heteromer score”) is a new bioinformatic tool that is independent of the method used to collect the data, requires no special sample preparation and can be used retrospectively on old datasets. It can be used for quality control, to indicate when a change becomes significant or identify complexes whose stoichiometry has been perturbed during the experiment. 相似文献
174.
Luis Toronjo-Urquiza Adelina E. Acosta-Martin David C. James Tibor Nagy Robert J. Falconer 《Biotechnology progress》2020,36(3):e2940
The effect of the addition of resveratrol to cell culture media during the production of monoclonal antibodies was investigated. Treatments of Chinese hamster ovary (CHO) cells expressing immunoglobulin G (IgG) with 25 and 50 μM resveratrol showed that resveratrol was capable of slowing cell growth while almost doubling cell-specific productivity to 4.7 ± 0.6 pg IgG/cell·day, resulting in up to a 1.37-fold increase of the final IgG titer. A resveratrol concentration of 50 μM slowed the progression through the cell cycle temporarily by trapping cells in the S-phase. Cation exchange chromatography showed no significant difference in the composition of acidic or basic IgG species and size exclusion chromatography indicated no change in fragmentation or aggregation of the recombinant IgG in the treatment groups. Resveratrol could be used as a chemical additive to CHO media where it would enhance IgG productivity and provide a degree of protection against hydroxyl and superoxide free radicals, expanding the range of options for process improvement available to monoclonal antibody manufacturers. 相似文献
175.
Marisa Liliana Fernández Maria Elena Marson Juan Carlos Ramirez Guido Mastrantonio Alejandro Gabriel Schijman Jaime Altcheh Adelina Rosa Riarte Facundo García Bournissen 《Memórias do Instituto Oswaldo Cruz》2016,111(3):218-221
Pharmacological treatment of Chagas disease with benznidazole (BNZ) is effective in
children in all stages, but it is controversial in chronically infected adults. We
report the pharmacokinetics and pharmacodynamics in six adult patients with Chagas
disease treated with the new BNZ formulation (ABARAX®) in doses between
2.5-5.5 mg/Kg/day. All but one patient had plasmatic BNZ concentrations within the
expected range. All patients finalised treatment with nondetectable
Trypanosoma cruziquantitative polymerase chain reaction, which
remained nondetectable at the six month follow-up. Our data suggests parasitological
responses with the new BNZ and supports the hypothesis that treatment protocols with
lower BNZ doses may be effective. 相似文献
176.
Adelina Vázquez Pilar Rodríguez Javier C. Torron 《Journal of Plant Growth Regulation》1983,2(1-4):117-120
The root differentiating properties of various benzyl alcohols were studied using a bean rooting test. The results showed that the methoxy derivatives enhanced rooting more efficiently than did the hydroxy derivatives. Only 4-hydroxybenzyl alcohol inhibited rooting, but the addition of IAA at 10?5M nullified the inhibition. Root promotion by the alcohols with a hydroxy group in the o-or p-position was increased by the addition of IAA. Auxin did not modify the rooting stimulation caused by the methoxy derivatives. The position of the methoxy group did not influence the rooting activity. 相似文献
177.
Manuel Ruiz Perez Adelina Valero Saez 《Human ecology: an interdisciplinary journal》1990,18(2):187-202
Existing transhumance practices in the Gredos Mountains are studied and the origin, destination, number of livestock, livestock raiser characteristics, and environmental variables of winter and summer pasture areas and drovers roads are analyzed. The important ecological, economic, and cultural role of transhumance is emphasized and attention is drawn to the need for its support and conservation. 相似文献