全文获取类型
收费全文 | 588篇 |
免费 | 45篇 |
专业分类
633篇 |
出版年
2021年 | 7篇 |
2016年 | 7篇 |
2015年 | 11篇 |
2014年 | 16篇 |
2013年 | 21篇 |
2012年 | 25篇 |
2011年 | 17篇 |
2010年 | 10篇 |
2009年 | 14篇 |
2008年 | 15篇 |
2007年 | 12篇 |
2006年 | 23篇 |
2005年 | 17篇 |
2004年 | 21篇 |
2003年 | 13篇 |
2002年 | 12篇 |
2001年 | 8篇 |
2000年 | 23篇 |
1999年 | 8篇 |
1998年 | 14篇 |
1997年 | 7篇 |
1996年 | 7篇 |
1995年 | 8篇 |
1994年 | 7篇 |
1993年 | 6篇 |
1992年 | 11篇 |
1991年 | 15篇 |
1990年 | 12篇 |
1989年 | 9篇 |
1988年 | 12篇 |
1987年 | 15篇 |
1986年 | 9篇 |
1985年 | 7篇 |
1984年 | 13篇 |
1983年 | 11篇 |
1979年 | 6篇 |
1978年 | 9篇 |
1977年 | 7篇 |
1976年 | 8篇 |
1975年 | 9篇 |
1974年 | 6篇 |
1973年 | 6篇 |
1971年 | 10篇 |
1970年 | 8篇 |
1969年 | 6篇 |
1968年 | 6篇 |
1967年 | 6篇 |
1966年 | 5篇 |
1953年 | 5篇 |
1942年 | 7篇 |
排序方式: 共有633条查询结果,搜索用时 8 毫秒
101.
Hideo Kimura Samuel A. Burstein David Thorning Jerry S. Powell Laurence A. Harker Philip J. Fialkow John W. Adamson 《Journal of cellular physiology》1984,118(1):87-96
The basic culture requirements and several physical characteristics were defined for megakaryocytic colony-forming cells (CFU-M) from normal human marrow growing in methylcellulose. Ficoll-hypaque separated mononuclear cells from human, marrow gave rise to megakaryocytic colonies in the presence of normal human plasma and phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM). Their identity as megakaryocytic colonies was confirmed by immunofluorescence staining with a monoclonal antibody to human factor VIII antigen and by electron microscopy of individually harvested colonies. Demonstration of the single-cell origin of the colonies was provided by analysis of the glucose-6-phosphate dehydrogenase (G-6-PD) enzyme type of individually harvested colonies grown from a G-6-PD heterozygote. The colonies grew best in heparinized or citrated plasma as opposed to serum. Detailed studies suggested that platelet-release products were responsible for this difference. Tritiated thymidine suicide studies showed that the percentage of CFU-M in DNA synthesis was 23 ± 8% (n = 10). The modal velocity sedimentation rate of CFU-M was 4.9 ± 0.6 mm/hr (n = 4) while that of concurrently studied granulocyte/macrophage colony-forming cells (CFU-GM) was 5.7 ± 0.5 mm/hr. Examination of the PHA-LCM dose-response characteristics suggested the presence in the conditioned medium of an inhibitor to megakaryocyte colony growth which was partially removed by chromatography of the medium on Sephadex G-100. The resulting conditioned medium increased the cloning efficiency for CFU-M compared with that with crude PHA-LCM (15.3 ± 7.0 and 8.2 ± 5.3/105 marrow cells, respectively). 相似文献
102.
103.
104.
105.
106.
107.
108.
109.
A Grover H Houlden M Baker J Adamson J Lewis G Prihar S Pickering-Brown K Duff M Hutton 《The Journal of biological chemistry》1999,274(21):15134-15143
110.