全文获取类型
收费全文 | 5254篇 |
免费 | 656篇 |
国内免费 | 3篇 |
出版年
2022年 | 47篇 |
2021年 | 71篇 |
2018年 | 70篇 |
2017年 | 49篇 |
2016年 | 108篇 |
2015年 | 149篇 |
2014年 | 171篇 |
2013年 | 177篇 |
2012年 | 268篇 |
2011年 | 249篇 |
2010年 | 146篇 |
2009年 | 150篇 |
2008年 | 174篇 |
2007年 | 203篇 |
2006年 | 183篇 |
2005年 | 189篇 |
2004年 | 148篇 |
2003年 | 175篇 |
2002年 | 169篇 |
2001年 | 180篇 |
2000年 | 170篇 |
1999年 | 131篇 |
1998年 | 77篇 |
1997年 | 57篇 |
1996年 | 65篇 |
1995年 | 51篇 |
1994年 | 66篇 |
1993年 | 47篇 |
1992年 | 109篇 |
1991年 | 114篇 |
1990年 | 97篇 |
1989年 | 121篇 |
1988年 | 85篇 |
1987年 | 107篇 |
1986年 | 81篇 |
1985年 | 92篇 |
1984年 | 76篇 |
1983年 | 62篇 |
1982年 | 60篇 |
1981年 | 52篇 |
1980年 | 50篇 |
1979年 | 73篇 |
1978年 | 48篇 |
1977年 | 52篇 |
1974年 | 59篇 |
1973年 | 58篇 |
1972年 | 68篇 |
1970年 | 52篇 |
1969年 | 54篇 |
1968年 | 46篇 |
排序方式: 共有5913条查询结果,搜索用时 31 毫秒
91.
92.
93.
K. H. Adams 《Biophysical journal》1973,13(3):209-217
Equations of mechanical equilibrium are applied to the erythrocyte membrane in the normal, hypotonically swollen, and sphered configurations. The hydrostatic pressure drop across the normal cell membrane is shown to be zero for all biconcave shapes if the membrane thickness is uniform. This result leads to the conclusion that the membrane tension is uniform and is a function of membrane potential. A two-dimensional fluid film model for the membrane is introduced to describe the unusual deformability of the erythrocyte during sphering in hypotonic solutions. The model predicts a smooth transition from the biconcave shape to a perfect sphere. 相似文献
94.
95.
96.
Inducible degradation of hydroxyproline in Pseudomonas putida: pathway regulation and hydroxyproline uptake 总被引:3,自引:0,他引:3 下载免费PDF全文
Studies in Pseudomonas putida of the inducible degradation of hydroxyproline to alpha-ketoglutarate have indicated that either of the two epimers, hydroxy-l-proline or allohydroxy-d-proline, acts as an inducer of all the pathway enzymes. In a mutant lacking the first enzyme of the sequence, hydroxyproline-2-epimerase, which interconverts these two hydroxyproline epimers, either epimer is still equally active as an inducer of the remaining three enzymes, suggesting that each epimer has intrinsic inducer activity. The second and third enzymes of the sequence were induced coordinately. The induction process appeared to be insensitive to catabolite repression under a number of experimental conditions. The induced enzymes were stable even under conditions of nitrogen starvation and other conditions designed to increase protein turnover. In addition to inducing the degradative enzymes, the two hydroxyproline epimers were also found to induce an uptake system that concentrates hydroxyproline intracellularly. Either amino acid induced the uptake system for its epimer as well as for itself. 相似文献
97.
Proteolysis and myelin breakdown: a review of recent histochemical and biochemical studies 总被引:9,自引:0,他引:9
Synopsis Proteins are important constituents of the myelin sheath and serve to maintain its structural integrity. One of the protein components is susceptible to tryptic digestion and may be regarded as a particularly vulnerable part of the myelin sheath. The initial events in myelin breakdown may involve disruption of lipid-protein attachments followed later by chemical degradation of released lipids.In Wallerian degeneration the activity of proteolytic enzymes increases by 12 hr after nerve section. Proteolytic enzyme activity increases in the prodromal phase of diphtheritic neuropathy. Extracts of degenerating nerve cause proteolysis of normal myelin with loss of trypanophilic basic protein and lipid; selective loss of basic protein occurs very early in Wallerian degeneration and has also been found in and around plaques of multiple sclerosis. Proteolytic activity is increased at the edges of active multiple sclerosis lesions. It has been shown that the basic encephalitogenic protein is susceptible to digestion by neural proteases, yielding an active encephalitogenic fragment.It is inferred from these collective observations that proteases play an important role in early myelin breakdown and may also be implicated in the pathogenesis of multiple sclerosis plaques by digesting basic protein, by releasing lipid from its attachment to such protein, and by liberating an active encephalitogenic peptide. The factors responsible for the activation and release of proteases remain unknown.Research Associate supported by the Multiple Sclerosis Society. 相似文献
98.
Developmental potential of single blastomeres of the rabbit egg 总被引:2,自引:0,他引:2
99.
100.
A new glycolipid sulfate ester in Halobacterium cutirubrum 总被引:7,自引:0,他引:7