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891.
Jose Henrique Pereira Richard A. Heins Daniel L. Gall Ryan P. McAndrew Kai Deng Keefe C. Holland Timothy J. Donohue Daniel R. Noguera Blake A. Simmons Kenneth L. Sale John Ralph Paul D. Adams 《The Journal of biological chemistry》2016,291(19):10228-10238
There has been great progress in the development of technology for the conversion of lignocellulosic biomass to sugars and subsequent fermentation to fuels. However, plant lignin remains an untapped source of materials for production of fuels or high value chemicals. Biological cleavage of lignin has been well characterized in fungi, in which enzymes that create free radical intermediates are used to degrade this material. In contrast, a catabolic pathway for the stereospecific cleavage of β-aryl ether units that are found in lignin has been identified in Sphingobium sp. SYK-6 bacteria. β-Aryl ether units are typically abundant in lignin, corresponding to 50–70% of all of the intermonomer linkages. Consequently, a comprehensive understanding of enzymatic β-aryl ether (β-ether) cleavage is important for future efforts to biologically process lignin and its breakdown products. The crystal structures and biochemical characterization of the NAD-dependent dehydrogenases (LigD, LigO, and LigL) and the glutathione-dependent lyase LigG provide new insights into the early and late enzymes in the β-ether degradation pathway. We present detailed information on the cofactor and substrate binding sites and on the catalytic mechanisms of these enzymes, comparing them with other known members of their respective families. Information on the Lig enzymes provides new insight into their catalysis mechanisms and can inform future strategies for using aromatic oligomers derived from plant lignin as a source of valuable aromatic compounds for biofuels and other bioproducts. 相似文献
892.
Inter and intra‐population phenotypic and genotypic structuring in the European whitefish Coregonus lavaretus,a rare freshwater fish in Scotland
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C. E. Adams C. W. Bean J. A. Dodd A. Down E. C. Etheridge A. R. D. Gowans O. Hooker R. Knudsen A. A. Lyle I. J. Winfield K. Præbel 《Journal of fish biology》2016,88(2):580-594
This study revealed between‐lake genetic structuring between Coregonus lavaretus collected from the only two native populations of this species in Scotland, U.K. (Lochs Eck and Lomond) evidenced by the existence of private alleles (12 in Lomond and four in Eck) and significant genetic differentiation (FST = 0·056) across 10 microsatellite markers. Juvenile C. lavaretus originating from eggs collected from the two lakes and reared in a common‐garden experiment showed clear phenotypic differences in trophic morphology (i.e. head and body shape) between these populations indicating that these characteristics were, at least partly, inherited. Microsatellite analysis of adults collected from different geographic regions within Loch Lomond revealed detectable and statistically significant but relatively weak genetic structuring (FST = 0·001–0·024) and evidence of private alleles related to the basin structure of the lake. Within‐lake genetic divergence patterns suggest three possibilities for this observed pattern: (1) differential selection pressures causing divergence into separate gene pools, (2) a collapse of two formerly divergent gene pools and (3) a stable state maintained by balancing selection forces resulting from spatial variation in selection and lake heterogeneity. Small estimates of effective population sizes for the populations in both lakes suggest that the capacity of both populations to adapt to future environmental change may be limited. 相似文献
893.
J. Barry M. Newton J. A. Dodd M. C. Lucas P. Boylan C. E. Adams 《Journal of fish biology》2016,88(2):676-689
The unimpeded downstream movement patterns and migration success of small female and male Anguilla anguilla through a catchment in north‐west Europe were studied using an acoustic hydrophone array along the River Finn and into the Foyle Estuary in Ireland. Twenty silver‐stage A. anguilla (total length, LT, range: 332–520 mm) were trapped 152 km upstream from a coastal marine sea‐lough outlet and internally tagged with acoustic transmitters of which 19 initiated downstream migration. Migration speed was highly influenced by river flow within the freshwater (FW) compartment. Anguilla anguilla activity patterns were correlated with environmental influences; light, tidal direction and lunar phase all influenced the initiation of migration of tagged individuals. Migration speed varied significantly between upstream and lower river compartments. Individuals migrated at a slower speed in transitional water and sea‐lough compartments compared with the FW compartment. While 88·5% survival was recorded during migration through the upper 121 km of the river and estuary, only 26% of A. anguilla which initiated downstream migration were detected at the outermost end of the acoustic array. Telemetry equipment functioned efficiently, including in the sea‐lough, so this suggests high levels of mortality during sea‐lough migration, or less likely, long‐term sea‐lough residence by silver A. anguilla emigrants. This has important implications for eel management plans. 相似文献
894.
895.
896.
Growth temperature impact on leaf form and function in Arabidopsis thaliana ecotypes from northern and southern Europe 总被引:1,自引:0,他引:1
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897.
I. Moroenyane K. Dong D. Singh S. B. M. Chimphango J. M. Adams 《Evolutionary ecology》2016,30(4):685-701
In many ecosystems, the factors that determine landscape-scale community structure of soil nematodes are poorly understood. We were interested in discovering whether deterministic or stochastic factors dominate nematode community variation. We used a novel metagenetic approach to investigate variation in nematode community structure in the Fynbos vegetation of South Africa. We compared 23 samples of soil nematode communities from five different Fynbos landscapes. Nematode DNA was 454-pyrosequenced for the 18S rRNA gene. We investigated the community structure, diversity, and the relative role of both deterministic (niche-based) and neutral processes may play in delimiting the nematode phylogenetic community structure in different Fynbos vegetation types. Nematode diversity showed no relationship to any measured soil parameter. The phylogenetic signal showed that more closely related types of nematodes in the Fynbos tended to co-occur more often than would be expected by chance, demonstrating that (a) closely related lineages occupy similar niches spatially and (b) community variation is influenced more by determinism than stochasticity. The standardised beta mean nearest taxon distance (ses.βMNTD) index showed no association with vegetation type. Both ses.NTI (nearest taxon index) and ses.βMNTD deviated significantly from null models, indicating that deterministic processes were important in the assembly of nematode communities. Furthermore, at local scale, the ses.NTI was significantly higher than null expectations, indicating that co-occurrence of related nematode lineages is determined by the differences in environmental conditions across the sites. We conclude that in the Fynbos there is niche overlap between closely related types of nematodes, that nematode speciation tends to occur conservatively into closely related niches, and that the phylogenetic community structure reveals that deterministic (rather than stochastic) processes are more important in delimiting the community assembly. 相似文献
898.
In almost all bacteria, cell division is co‐ordinated by the essential tubulin homologue FtsZ and represents an attractive but as yet unexploited target for new antibiotics. The benzamides, e.g. PC190723, are potent FtsZ inhibitors that have the potential to yield an important new class of antibiotic. However, the evolution of resistance poses a challenge to their development. Here we show that a collection of PC190723‐resistant and ‐dependent strains of Staphylococcus aureus exhibit severe growth and morphological defects, questioning whether these ftsZ mutations would be clinically relevant. Importantly, we show that the most commonly isolated substitution remains sensitive to the simplest benzamide 3‐MBA and likely works by occluding compound binding. Extending this analysis to Bacillus subtilis, we isolated a novel benzamide‐dependent strain that divides using unusual helical division events. The ftsZ mutation responsible encodes the substitution of a highly conserved residue, which lies outside the benzamide‐binding site and forms part of an interface between the N‐ and C‐terminal domains that we show is necessary for normal FtsZ function. Together with an intragenic suppressor mutation that mimics benzamide binding, the results provide genetic evidence that benzamides restrict conformational changes in FtsZ and also highlights their utility as tools to probe bacterial division. 相似文献
899.
900.
Effect of nitrogen limitation on foliar antioxidants in relationship to other metabolic characteristics 总被引:13,自引:0,他引:13
Barry A. Logan Barbara Demmig-Adams Todd N. Rosenstiel William W. Adams III 《Planta》1999,209(2):213-220
The long-term effect of limiting soil nitrogen (N) availability on foliar antioxidants, thermal energy dissipation, photosynthetic
and respiratory electron transport, and carbohydrates was investigated in Spinacia oleracea L. Starch, sucrose, and glucose accumulated in leaves of N-limited spinach at predawn, consistent with a downregulation of
chloroplast processes by whole-plant sink limitation in response to a limited supply of N-based macromolecules throughout
the plant. On a leaf-area or dry-weight basis, levels of chlorophyll, carotenoid pools, photosynthetic electron transport
capacity, as well as activities for the predominantly chloroplast-localized antioxidant enzymes ascorbate peroxidase (EC 1.11.1.11)
and glutathione reductase (EC 1.6.4.2) were much lower in N-limited versus N-replete plants. When expressed on a chlorophyll
basis, foliar levels of all of these parameters were similar in N-replete versus N-limited plants. However, on a total-protein
basis, antioxidant enzyme activities were higher in N-limited plants. Nitrogen-limited spinach showed higher levels of thermal
energy dissipation and of zeaxanthin and antheraxanthin at midday, as well as slightly higher ascorbate contents relative
to chlorophyll. These results indicate that strong, long-term N limitation led not only to alterations in the balance between
different processes but also to an overall downregulation of light collection, photosynthetic electron transport capacity,
and chloroplast-based antioxidant enzymes. This is further supported by the finding that glucose-feeding of excised leaves
led to strong concomitant decreases in photosynthetic electron transport capacity and ascorbate peroxidase activity. On a
leaf-area basis, neither superoxide dismutase (EC 1.15.1.1) activity nor dark repiration rates showed a treatment effect.
This indicates that overall mitochondrial electron transport activity does not decrease under long-term N limitation and is
consistent with localization of an important fraction of foliar superoxide dismutase in mitochondria.
Received: 19 March 1999 / Accepted: 13 April 1999 相似文献