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71.
Rizky Pasthika Kirana Kumar Gaurav Sanu Arora Gerlinde Wiesenberger Maria Doppler Sebastian Michel Simone Zimmerl Magdalena Matic Chinedu E. Eze Mukesh Kumar Ajla Topuz Marc Lemmens Rainer Schuhmacher Gerhard Adam Brande B. H. Wulff Hermann Buerstmayr Barbara Steiner 《Plant biotechnology journal》2023,21(1):109-121
Aegilops tauschii is the diploid progenitor of the wheat D subgenome and a valuable resource for wheat breeding, yet, genetic analysis of resistance against Fusarium head blight (FHB) and the major Fusarium mycotoxin deoxynivalenol (DON) is lacking. We treated a panel of 147 Ae. tauschii accessions with either Fusarium graminearum spores or DON solution and recorded the associated disease spread or toxin-induced bleaching. A k-mer-based association mapping pipeline dissected the genetic basis of resistance and identified candidate genes. After DON infiltration nine accessions revealed severe bleaching symptoms concomitant with lower conversion rates of DON into the non-toxic DON-3-O-glucoside. We identified the gene AET5Gv20385300 on chromosome 5D encoding a uridine diphosphate (UDP)-glucosyltransferase (UGT) as the causal variant and the mutant allele resulting in a truncated protein was only found in the nine susceptible accessions. This UGT is also polymorphic in hexaploid wheat and when expressed in Saccharomyces cerevisiae only the full-length gene conferred resistance against DON. Analysing the D subgenome helped to elucidate the genetic control of FHB resistance and identified a UGT involved in DON detoxification in Ae. tauschii and hexaploid wheat. This resistance mechanism is highly conserved since the UGT is orthologous to the barley UGT HvUGT13248 indicating descent from a common ancestor of wheat and barley. 相似文献
72.
Elnur Babayev Chanakarn Suebthawinkul Dilan Gokyer Wendena S. Parkes Felipe Rivas Mary Ellen Pavone Adam R. Hall Michele T. Pritchard Francesca E. Duncan 《Aging cell》2023,22(11):e14004
Reproductive aging is associated with ovulatory defects. Age-related ovarian fibrosis partially contributes to this phenotype as short-term treatment with anti-fibrotic compounds improves ovulation in reproductively old mice. However, age-dependent changes that are intrinsic to the follicle may also be relevant. In this study, we used a mouse model to demonstrate that reproductive aging is associated with impaired cumulus expansion which is accompanied by altered morphokinetic behavior of cumulus cells as assessed by time-lapse microscopy. The extracellular matrix integrity of expanded cumulus–oocyte complexes is compromised with advanced age as evidenced by increased penetration of fluorescent nanoparticles in a particle exclusion assay and larger open spaces on scanning electron microscopy. Reduced hyaluronan (HA) levels, decreased expression of genes encoding HA-associated proteins (e.g., Ptx3 and Tnfaip6), and increased expression of inflammatory genes and matrix metalloproteinases underlie this loss of matrix integrity. Importantly, HA levels are decreased with age in follicular fluid of women, indicative of conserved reproductive aging mechanisms. These findings provide novel mechanistic insights into how defects in cumulus expansion contribute to age-related infertility and may serve as a target to extend reproductive longevity. 相似文献
73.
Amino Acids - Protein arginine N-methyltransferases (PRMTs) have emerged as important actors in the eukaryotic stress response with implications in human disease, aging, and cell signaling.... 相似文献
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76.
Anna Nasulewicz Andrzej Mazur Adam Opolski 《Journal of trace elements in medicine and biology》2004,18(1):1-8
The formation of new blood vessels is the initial step in progressive tumour development and metastasis. The first stage in tumour angiogenesis is the activation of endothelial cells. Copper ions stimulate proliferation and migration of endothelial cells. It has been shown that serum copper concentration increases as the cancer disease progresses and correlates with tumour incidence and burden. Copper ions also activate several proangiogenic factors, e.g., vascular endothelial growth factor, basic fibroblast growth factor, tumour necrosis factor alpha and interleukin 1. This review concerns a brief introduction into the basics of tumour blood vessel development as well as the regulatory mechanisms of this process. The role of copper ions in tumour angiogenesis is discussed. The new antiangiogenic therapies based on a reduction of copper levels in tumour microenvironment are reviewed. 相似文献
77.
Carbon : nitrogen : phosphorus (C : N : P) stoichiometry can underlie physiological and life history characteristics that shape ecological interactions. Despite its potential importance, there is much to learn about the causes and consequences of stoichiometric variation in terrestrial consumers. Here we show that treehoppers (Publilia modesta) tended by ants (Formica obscuripes) contained lower N concentrations than treehoppers on plants from which ants were excluded. Ant presence also affected nutrient concentrations in host plants: on plants with ants, leaves contained uniformly low concentrations of N; on plants without ants, N concentrations were low only in the few leaves fed upon by treehoppers at the time of collection. We suggest treehopper feeding reduces leaf nutrient levels and ants positively affect treehopper abundance, producing a top–down effect on plant quality. Determining the causes of these stoichiometric changes should help elucidate factors guiding the dynamics of conditional mutualisms between ants and homopterans. 相似文献
78.
Measuring Success: Evaluating the Restoration of a Grassy Eucalypt Woodland on the Cumberland Plain, Sydney, Australia 总被引:10,自引:0,他引:10
Abstract We compared the floristic composition and structure of restoration areas of eucalypt woodland with untreated pasture (control) and remnant vegetation (reference) in western Sydney. The restored areas comprised over 1,000 ha of abandoned pasture, which had been treated to reduce weeds and planted with seedlings of 26 native plant species raised from seed obtained locally from remnant vegetation. Plantings were carried out 0–9 years ago. Floristic composition was measured in quadrats using frequency scores and cover abundance. As far as possible treatments and restoration ages were replicated across sites. Ordination and analyses of similarity failed to distinguish the composition of restored vegetation from that of untreated pasture, which were both significantly different from that of remnant vegetation. There was a weak compositional trend with age of restored vegetation, but this was not in the direction of increasing resemblance to remnant vegetation. There was some evidence for convergence in structural features of restored with remnant vegetation, but this was at least partly attributed to plant growth. Subject to constraints imposed by the sampling design, environmental factors, and spatial variation were discounted as explanations for the results. The results therefore suggest either failure of restoration treatments or a restoration trajectory that is too slow to detect within 10 years of establishment. Our conclusions agree with those of similar studies in other ecosystems and support: (1) the need to monitor restoration projects against ecological criteria with rigorous sampling designs and analytical methods, (2) further development of restoration methods, and (3) regulatory approaches that seek to prevent damage to ecosystems rather than those predicated on replacing losses with reconstructed ecosystems. 相似文献
79.
80.
Irina Zaitseva Vjacheslav Zaitsev Graeme Card Kirill Moshkov Benjamin Bax Adam Ralph Peter Lindley 《Journal of biological inorganic chemistry》1996,1(1):15-23
The X-ray structure of human serum ceruloplasmin has been solved at a resolution of 3.1?Å. The structure reveals that the molecule is comprised of six plastocyanin-type domains arranged in a triangular array. There are six copper atoms; three form a trinuclear cluster sited at the interface of domains 1 and 6, and there are three mononuclear sites in domains 2, 4 and 6. Each of the mononuclear coppers is coordinated to a cysteine and two histidine residues, and those in domains 4 and 6 also coordinate to a methionine residue; in domain 2, the methionine is replaced by a leucine residue which may form van der Waals type contacts with the copper. The trinuclear centre and the mononuclear copper in domain 6 form a cluster essentially the same as that found in ascorbate oxidase, strongly suggesting an oxidase role for ceruloplasmin in the plasma. 相似文献