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121.
Matilde Fernández Bertrand Morel Andrés Corral‐Lugo Tino Krell 《Molecular microbiology》2016,99(1):34-42
Chemotaxis is an essential mechanism that enables bacteria to move toward favorable ecological niches. Escherichia coli, the historical model organism for studying chemotaxis, has five well‐studied chemoreceptors. However, many bacteria with different lifestyle have more chemoreceptors, most of unknown function. Using a high throughput screening approach, we identified a chemoreceptor from Pseudomonas putida KT2440, named McpH, which specifically recognizes purine and its derivatives, adenine, guanine, xanthine, hypoxanthine and uric acid. The latter five compounds form part of the purine degradation pathway, permitting their use as sole nitrogen sources. Isothermal titration calorimetry studies show that these six compounds bind McpH‐Ligand Binding Domain (LBD) with very similar affinity. In contrast, non‐metabolizable purine derivatives (caffeine, theophylline, theobromine), nucleotides, nucleosides or pyrimidines are unable to bind McpH‐LBD. Mutation of mcpH abolished chemotaxis toward the McpH ligands identified – a phenotype that is restored by complementation. This is the first report on bacterial chemotaxis to purine derivatives and McpH the first chemoreceptor described that responds exclusively to intermediates of a catabolic pathway, illustrating a clear link between metabolism and chemotaxis. The evolution of McpH may reflect a saprophytic lifestyle, which would have exposed the studied bacterium to high concentrations of purines produced by nucleic acid degradation. 相似文献
122.
Jaime García-Mena Selvasankar Murugesan Ashael Alfredo Pérez-Muñoz Matilde García-Espitia Otoniel Maya Monserrat Jacinto-Montiel Giselle Monsalvo-Ponce Alberto Piña-Escobedo Lilianha Domínguez-Malfavón Marlenne Gómez-Ramírez Elsa Cervantes-González María Teresa Núñez-Cardona 《Microbial ecology》2016,72(1):70-84
123.
Renata Barsacchi Paolo Camici Ubaldo Bottigli Piero A. Salvadori Gualtiero Pelosi Matilde Maiorino Fulvio Ursini 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1983,762(2):241-247
The isolated perfused rat heart emits a spontaneous ultraweak chemiluminescence. When the perfusion is stopped, light emission decreases, indicating the dependency of this phenomenon on aerobic metabolism. Emitted chemiluminescence was markedly enhanced following perfusion with 0.05 mM H2O2 or cumene hydroperoxide or tert-butyl hydroperoxide; substitution of O2 for N2 in the gassing mixture of the perfusion media significantly lowered photon emission. Lipid peroxidation, which is known to be associated with chemiluminescence, was evaluated by HPLC analysis of peroxidized and unperoxidized heart phosphatidylcholines. During hydroperoxide perfusion, coronary flow and heart rate progressively decreased, while lactic dehydrogenase was released after complete cardiac arrest. The resultant morphology of this damage corresponds to the so-called ‘stone heart’, a pattern already described in both human and experimental pathology. 相似文献
124.
A combined chromosome and C-heterochromatin polymorphism in pair 12 in the complement of the newt species, T. italicus is described. The C-heterochromatin polymorphism is presumably due to a loss in the proximal C-band, whereas the chromosomal polymorphism has its origin in two different independent pericentric inversions both including the centromere and the proximal C-band of chromosome 12. The double-inversion polymorphism has a wide distribution over the range and follows a clear bipolarity between a northern area where the karyotype is homomorphic for the standard type of pair 12 (ST/ST) and an opposite area where the ST type is completely replaced by variant M1 and M2 metacentric chromosomes 12. Various karyophylogenies are possible, but the simplest and the most probable presumes an ancestral karyotype of ST/ST and a mechanism of gradual replacement of the heterobrachial chromosome ST by two independent pericentric inversions. The present data are discussed in relation to existing theories on karyological evolution of Urodeles and the functional significance of telocentric chromosomes suggested by Sessions et al. (1982). 相似文献
125.
Matilde H. Navarrete Gardenia González-Gil S. Martín-Hurtado J. F. López-Sáez 《Protoplasma》1984,121(1-2):138-145
Summary Cell distribution in different compartments of the cell cycle (G1, early, middle and late S, G2 and mitosis) has been studied during treatment with 0.5 mM 5-aminouracil and recovery inAllium cepa L. root meristems by cytophotometric and autoradiographic methods. At optimum conditions for obtaining mitotic synchronization, 5-aminouracil gives rise to cell accumulation in the S period, preferentially in its middle zone where the relative DNA content is 2.8 ± 0.1 C. After a 14-hour treatment 33% of the proliferative population is accumulated in this particular region.During recovery, a drastic reduction of the S phase and a clear increase of the mitotic frequency are the most important events observed. Apparently, the removal of the drug frees the blockage and the accumulated cells complete their interphase making up the mitotic wave. 相似文献
126.
Ana S. Fernandes João Serejo Jorge Gaspar Fátima Cabral Ana F. Bettencourt José Rueff Matilde Castro Judite Costa Nuno G. Oliveira 《Cell biology and toxicology》2010,26(2):91-101
Oxidative cell injury could be induced by different reactive oxygen species (ROS) operating in multiple pathways. The present
work is focused on three different models of oxidative stress: the xanthine/xanthine oxidase system (XXO), an extracellular
superoxide anion generator; tert-butylhydroperoxide (TBHP), an analogue of lipid hydroperoxides; and doxorubicin (Dox), an anticancer drug. Superoxide and
peroxyl radicals, among other ROS, could be effectively scavenged by MnTM-4-PyP, a polyfunctional catalytic antioxidant. In
this report, we have addressed the role of MnTM-4-PyP on the protection against the cytotoxicity induced by the three aforementioned
oxidants. The effect of MnTM-4-PyP (0.1–100 μM) was evaluated in V79 fibroblasts using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide reduction and the crystal violet assays, as well as the mitotic index. Also, the generation of intracellular
ROS was studied by the fluorescent probe dihydroethidium. MnTM-4-PyP has shown significant protective effects against the
cytotoxicity of XXO and TBHP, increasing the cell viability in approximately 40% and reducing the intracellular level of ROS.
However, no considerable protection occurred against Dox. The three oxidants caused a mitotic index reduction that was not
altered by MnTM-4-PyP. In summary, MnTM-4-PyP appears to be a promising agent for the protection against oxidative injury.
However, it has shown differential responses, reinforcing the need to study different experimental models for the adequate
evaluation of its potentialities as a catalytic antioxidant. 相似文献
127.
128.
Melania Maglio Matilde Tschon Laura Sicuro Roberta Lolli Milena Fini 《Journal of cellular physiology》2019,234(5):5420-5435
The increasing demand for reliable preclinical models and to reduce, refine and, if possible, replace animal studies have brought forth the development of complex tissue cultures in different research areas, including the musculoskeletal field. In this paper, we review the literature within last 10 years on the state of progress for in vitro models of osteochondral tissue cultures, taking into account the clinical relevance of the management and treatment of osteochondral lesions. According to the selected research criteria, 35 works, 27 of which with animal tissues and 8 with human tissues, resulted to be relevant for the purposes of this review. Data analyzed revealed a great heterogeneity among the proposed tissue culture models. The anatomical harvesting sites resulted to be mainly the knee stifle joint, both for animal (prevalently bovines) and human tissues derived from joint replacement surgery, and significant heterogeneity among culture conditions and media were found. To date, very few papers have focused on the set up of a reproducible in vitro model, applicable to a variety of studies, thus suggesting a relevant gap to fill in the development of advanced three-dimensional osteochondral culture models. 相似文献
129.
Chacón MR Fernández-Real JM Richart C Megía A Gómez JM Miranda M Caubet E Pastor R Masdevall C Vilarrasa N Ricard W Vendrell J 《Obesity (Silver Spring, Md.)》2007,15(3):664-672
Objective: Our goal was to test any association between human plasma circulating levels of monocyte chemoattractant protein‐1 (cMCP‐1) and insulin resistance and to compare monocyte chemoattractant protein‐1 (MCP‐1) adipose tissue gene expression and cMCP‐1 in relation with inflammatory markers. Research Methods and Procedures: cMCP‐1 was measured in n = 116 consecutive control male subjects to whom an insulin sensitivity (Si) test was performed. Circulating levels of soluble CD14, soluble tumor necrosis factor receptor type 2 (sTNFR2), soluble interleukin‐6 (sIL‐6), and adiponectin also were measured. Subcutaneous adipose tissue samples were obtained from n = 107 non‐diabetic and type 2 diabetic subjects with different degrees of obesity. Real‐time polymerase chain reaction was used to measure gene expression of MCP‐1, CD68, tumor necrosis factor‐α (TNF‐α), and its receptor TNFR2. Results: In the Si study, no independent effect of cMCP‐1 levels on insulin sensitivity was observed. In the expression study, in non‐diabetic subjects, MCP‐1 mRNA had a positive correlation with BMI (r = 0.407, p = 0.003), TNF‐α mRNA (r = 0.419, p = 0.002), and TNFR2 mRNA (r = 0.410, p = 0.003). In these subjects, cMCP‐1 was found to correlate with waist‐to‐hip ratio (r = 0.322, p = 0.048). In patients with type 2 diabetes, MCP‐1 mRNA was up‐regulated compared with non‐diabetic subjects. TNF‐α mRNA was found to independently contribute to MCP‐1 mRNA expression. In this group, CD68 mRNA was found to correlate with BMI (r = 0.455, p = 0.001). Discussion: cMCP‐1 is not associated with insulin sensitivity in apparently healthy men. TNF‐α is the inflammatory cytokine associated with MCP‐1 expression in subcutaneous adipose tissue. 相似文献