全文获取类型
收费全文 | 370篇 |
免费 | 31篇 |
专业分类
401篇 |
出版年
2023年 | 3篇 |
2022年 | 5篇 |
2021年 | 6篇 |
2020年 | 13篇 |
2019年 | 13篇 |
2018年 | 7篇 |
2017年 | 11篇 |
2016年 | 6篇 |
2015年 | 18篇 |
2014年 | 20篇 |
2013年 | 30篇 |
2012年 | 18篇 |
2011年 | 21篇 |
2010年 | 18篇 |
2009年 | 16篇 |
2008年 | 28篇 |
2007年 | 23篇 |
2006年 | 15篇 |
2005年 | 8篇 |
2004年 | 15篇 |
2003年 | 11篇 |
2002年 | 8篇 |
2001年 | 4篇 |
2000年 | 6篇 |
1999年 | 9篇 |
1998年 | 4篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1971年 | 2篇 |
1970年 | 2篇 |
1969年 | 1篇 |
1967年 | 1篇 |
1965年 | 2篇 |
排序方式: 共有401条查询结果,搜索用时 0 毫秒
81.
Nicholas K. Fletcher Pelayo Acevedo Jeremy S. Herman Joana Pauprio Paulo C. Alves Jeremy B. Searle 《Ecology and evolution》2019,9(24):14101-14113
Understanding the factors that contribute to the generation of reproductively isolated forms is a fundamental goal of evolutionary biology. Cryptic species are an especially interesting challenge to study in this context since they lack obvious morphological differentiation that provides clues to adaptive divergence that may drive reproductive isolation. Geographical isolation in refugial areas during glacial cycling is known to be important for generating genetically divergent populations, but its role in the origination of new species is still not fully understood and likely to be situation dependent. We combine analysis of 35,434 single‐nucleotide polymorphisms (SNPs) with environmental niche modeling (ENM) to investigate genomic and ecological divergence in three cryptic species formerly classified as the field vole (Microtus agrestis). The SNPs demonstrate high genomic divergence (pairwise FST values of 0.45–0.72) and little evidence of gene flow among the three field vole cryptic species, and we argue that genetic drift may have been a particularly important mechanism for divergence in the group. The ENM reveals three areas as potential glacial refugia for the cryptic species and differing climatic niches, although with spatial overlap between species pairs. This evidence underscores the role that glacial cycling has in promoting genetic differentiation and reproductive isolation by subdivision into disjunct distributions at glacial maxima in areas relatively close to ice sheets. Future investigation of the intrinsic barriers to gene flow between the field vole cryptic species is required to fully assess the mechanisms that contribute to reproductive isolation. In addition, the Portuguese field vole (M. rozianus) shows a high inbreeding coefficient and a restricted climatic niche, and warrants investigation into its conservation status. 相似文献
82.
83.
Range dynamics driven by Quaternary climate oscillations explain the distribution of introgressed mtDNA of Lepus timidus origin in hares from the Iberian Peninsula 下载免费PDF全文
84.
85.
86.
CO2 supply at 4% (v/v) in air increased the biooxidation of a gold concentrate (41% enargite, 43% pyrite) with Thiobacillus ferrooxidans in a 2-l bioreactor at 4% (w/v) solids concentration, 35°C and pH 2.4. Extraction increased from 21 to 69% for Fe, 19 to 25% for As, and 16 to 19% for Cu. Suspended biomass increased from 2.6·107 to 1.2·108 cells/ml. It is concluded that the biooxidation of this gold concentrate is limited by the rate of CO2 supply. © Rapid Science Ltd. 1998 相似文献
87.
88.
Nair Remya R. Corrochano Silvia Gasco Samanta Tibbit Charlotte Thompson David Maduro Cheryl Ali Zeinab Fratta Pietro Arozena Abraham Acevedo Cunningham Thomas J. Fisher Elizabeth M. C. 《Mammalian genome》2019,30(7-8):173-191
Mammalian Genome - Neurodegenerative disease encompasses a wide range of disorders afflicting the central and peripheral nervous systems and is a major unmet biomedical need of our time. There are... 相似文献
89.
Cristian A. Acevedo Paulo Díaz-Calderón Javier Enrione María J. Caneo Camila F. Palacios Caroline Weinstein-Oppenheimer Donald I. Brown 《Bioprocess and biosystems engineering》2015,38(4):777-785
Biomaterials based on crosslinked sponges of biopolymers have been extensively used as scaffolds to culture mammal cells. It is well known that single biopolymers show significant change over time due to a phenomenon called physical ageing. In this research, it was verified that scaffolds used for skin tissue engineering (based on gelatin, chitosan and hyaluronic acid) express an ageing-like phenomenon. Treatments based on ageing of scaffolds improve the behavior of skin-cells for tissue engineering purposes. Physical ageing of dry scaffolds was studied by differential scanning calorimetry and was modeled with ageing kinetic equations. In addition, the physical properties of wet scaffolds also changed with the ageing treatments. Scaffolds were aged up to 3 weeks, and then skin-cells (fibroblasts) were seeded on them. Results indicated that adhesion, migration, viability, proliferation and spreading of the skin-cells were affected by the scaffold ageing. The best performance was obtained with a 2-week aged scaffold (under cell culture conditions). The cell viability inside the scaffold was increased from 60 % (scaffold without ageing treatment) to 80 %. It is concluded that biopolymeric scaffolds can be modified by means of an ageing treatment, which changes the behavior of the cells seeded on them. The ageing treatment under cell culture conditions might become a bioprocess to improve the scaffolds used for tissue engineering and regenerative medicine. 相似文献
90.
Ruiz-Fons F Fernández-de-Mera IG Acevedo P Gortázar C de la Fuente J 《Applied and environmental microbiology》2012,78(8):2669-2676
Environmental factors may drive tick ecology and therefore tick-borne pathogen (TBP) epidemiology, which determines the risk to animals and humans of becoming infected by TBPs. For this reason, the aim of this study was to analyze the influence of environmental factors on the abundance of immature-stage Ixodes ricinus ticks and on the prevalence of two zoonotic I. ricinus-borne pathogens in natural foci of endemicity. I. ricinus abundance was measured at nine sites in the northern Iberian Peninsula by dragging the vegetation with a cotton flannelette, and ungulate abundance was measured by means of dung counts. In addition to ungulate abundance, data on variables related to spatial location, climate, and soil were gathered from the study sites. I. ricinus adults, nymphs, and larvae were collected from the vegetation, and a representative subsample of I. ricinus nymphs from each study site was analyzed by PCR for the detection of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum DNA. Mean prevalences of these pathogens were 4.0% ± 1.8% and 20.5% ± 3.7%, respectively. Statistical analyses confirmed the influence of spatial factors, climate, and ungulate abundance on I. ricinus larva abundance, while nymph abundance was related only to climate. Interestingly, cattle abundance rather than deer abundance was the main driver of B. burgdorferi sensu lato and A. phagocytophilum prevalence in I. ricinus nymphs in the study sites, where both domestic and wild ungulates coexist. The increasing abundance of cattle seems to increase the risk of other hosts becoming infected by A. phagocytophilum, while reducing the risk of being infected by B. burgdorferi sensu lato. Controlling ticks in cattle in areas where they coexist with wild ungulates would be more effective for TBP control than reducing ungulate abundance. 相似文献