Effects of Bacterial Inoculation to Immobilize Nickel in Wheat Grown on Ni-Contaminated Soil

Abstract

Plant growth stimulating bacteria are very effective in immobilization of metals and reducing their translocation in plants through precipitation, and adsorption. A pot experiment was conducted to investigate the effectiveness of chitosan- and hematite-modified biochar and bacterial inoculations on the immobilization of nickel (Ni) in polluted soil under wheat cultivation. Application of modified biochars and inoculation with Pseudomonas putida significantly increased both wheat root and shoot dry matter yields but decreased Ni phytoextraction efficiency. The Ni concentration, translocation factor and uptake in wheat shoot and root significantly decreased the application of either modified or unmodified biochars. Bacterial inoculation significantly decreased mean translocation factor and also root and shoot concentration and the uptake Ni in the shoot. Chitosan-modified biochar was the most influential treatment in decreasing Ni uptake by wheat followed by P. putida inoculation treatment. The results demonstrated positive effects of chitosan modified biochar and inoculation with P. putida in increasing dry matter yield and decreasing Ni uptake in wheat grown on Ni-contaminated soil. According to the results of present study, modified biochars application and bacterial inoculation are influential treatments which prevent Ni toxicity probably.     

Habitat utilisation and nesting behaviour of two sympatric weavers in Mbalmayo District,Cameroon

Avian ecology and behaviour data provides crucial information for bird conservation and management, but are still missing for many species, particularly in developing countries. This study examines habitat utilisation, nesting behaviour, anthropogenic disturbances and impacts on agricultural crops of Village Weaver Ploceus cucullatus and Vieillot’s Black Weaver Ploceus nigerrimus in Mbalmayo District, Cameroon. Abundance was estimated by nest counts and point counts of birds at 40 previously located colonies, where also tree metrics and anthropogenic disturbances were measured. In total, more than 1 700 birds of the two species were estimated within a 9 km2 study area, divided into two blocks and five different habitat types. Village Weavers were generally more abundant, especially in the urban habitat, whereas Vieillot’s Black Weavers were most numerous in the rural habitat. Among the tree species used for nesting, mango Mangifera indica and African border tree Newbouldia laevis had the highest nest counts, which also was positively related to canopy cover diameter. Traffic noise disturbance was associated with lower bird and nest counts, whereas the opposite was observed on farming disturbance. The relative impacts on agricultural crops were estimated for African oil palm Elaeis guineensis (38%), maize Zea mays (36%), Musa spp. (18%) and Mangifera indica (8%). The implications for long-term coexistence between farmland birds and humans are discussed, and the challenge is to minimise both kinds of bird–human interactions, i.e. anthropogenic disturbances and agricultural crop damage.     

Does aging affect the intestinal transport of riboflavin?

Riboflavin deficiency has been reported in older individuals. The cause of this deficiency is not know but could include a decrease in the intestinal absorptive capacity for riboflavin. Therefore, we examined the intestinal absorption of riboflavin in young (3 month) and old (26 month) rats. We used in vitro jejunal everted sacs. The kinetic parameters of riboflavin absorption disclosed apparent Km of 0.37 and 0.43 microM and Vmax of 37 and 38 pmole/g initial tissue wet wt/20 min in young and old rats, respectively. These data do not demonstrate an aging associated change in the intestinal transport capacity for riboflavin. If these results are extrapolated to man, it would mean that the deficiency of riboflavin found in the elderly is not due to its intestinal malabsorption. Therefore, other mechanisms must be sought to account for the deficiency of riboflavin seen in the elderly.     

Percutaneous transvenous retrieval of intracardiac port-a-cath catheter fragment: a case report

A female patient, 36 years of age, with a metastasised left breast cancer received several courses of chemotherapy for aggressive local tumour growth and multiple metastatic activity. In the current patient, surgical ablation of the left breast was carried out. Also loco-regional radio-therapy was conducted. To facilitate the administration of chemotherapy courses and prevent thrombophlebitis a vascular access port (port-a-cath) was surgically inserted via the right subclavian vein. After a few successful administrations of chemotherapeutic drugs the vascular port stopped functioning. It was demonstrated that a detached catheter fragment had dislodged into the right ventricle. Successful percutaneous, transvenous removal of the entrapped catheter fragment by the Gooseneck retrieval loop snare from the right ventricle was performed via the right femoral vein access. The procedure was uncomplicated and the patient tolerated the procedure well.     

Pulmonary vasodilator responses to vasoactive intestinal peptide in the cat

We investigated the effects of vasoactive intestinal peptide (VIP) in the feline pulmonary vascular bed under conditions of controlled pulmonary blood flow when pulmonary vascular tone was at base-line levels and when vascular resistance was elevated. Under base-line conditions, VIP caused small but significant reductions in lobar arterial pressure without affecting left atrial pressure. Decreases in lobar arterial pressure in response to VIP were greater and were dose related when lobar vascular resistance was increased by intralobar infusion of U 46619, a stable prostaglandin endoperoxide analogue. Acetylcholine and isoproterenol also caused significant decreases in lobar arterial pressure under base-line conditions, and responses to these agents were enhanced when lobar vascular tone was elevated. Moreover, when doses of these agents are expressed in nanomoles, acetylcholine and isoproterenol were more potent than VIP in decreasing lobar arterial pressure. Responses to VIP were longer in duration with a slower onset than were responses to acetylcholine or isoproterenol. Pulmonary vasodilator responses to VIP were unchanged by indomethacin, atropine, or propranolol. The present data demonstrate that VIP has vasodilator activity in the pulmonary vascular bed and that responses are dependent on the existing level of vasoconstrictor tone. These studies indicate that this peptide is less potent than acetylcholine or isoproterenol in dilating the feline pulmonary vascular bed and that responses to VIP are not dependent on a muscarinic or beta-adrenergic mechanism or release of a dilator prostaglandin.     

Ischemia reperfusion dysfunction changes model-estimated kinetics of myofilament interaction due to inotropic drugs in isolated hearts

Background  

The phase-space relationship between simultaneously measured myoplasmic [Ca²⁺] and isovolumetric left ventricular pressure (LVP) in guinea pig intact hearts is altered by ischemic and inotropic interventions. Our objective was to mathematically model this phase-space relationship between [Ca²⁺] and LVP with a focus on the changes in cross-bridge kinetics and myofilament Ca²⁺ sensitivity responsible for alterations in Ca²⁺-contraction coupling due to inotropic drugs in the presence and absence of ischemia reperfusion (IR) injury.     

Phenomic and genomic approaches to studying the inhibition of multiresistant Salmonella enterica by microcin J25

In livestock production, antibiotics are used to promote animal growth, control infections and thereby increase profitability. This practice has led to the emergence of multiresistant bacteria such as Salmonella, of which some serovars are disseminated in the environment. The objective of this study is to evaluate microcin J25 as an inhibitor of Salmonella enterica serovars of various origins including human, livestock and food. Among the 116 isolates tested, 37 (31.8%) were found resistant to at least one antibiotic, and 28 were multiresistant with 19 expressing the penta-resistant phenotype ACSSuT. Microcin J25 inhibited all isolates, with minimal inhibitory concentration values ranging from 0.06 μg/ml (28.4 nM) to 400 μg/ml (189 μM). Interestingly, no cross-resistance was found between microcin J25 and antibiotics. Multiple sequence alignments of genes encoding for the different proteins involved in the recognition and transport of microcin J25 showed that only ferric-hydroxamate uptake is an essential determinant for susceptibility of S. enterica to microcin J25. Examination of Salmonella strains exposed to microcin J25 by transmission electronic microscopy showed for the first-time involvement of a pore formation mechanism. Microcin J25 was a strong inhibitor of several multiresistant isolates of Salmonella and may have a great potential as an alternative to antibiotics.