全文获取类型
收费全文 | 941篇 |
免费 | 60篇 |
国内免费 | 5篇 |
出版年
2024年 | 2篇 |
2023年 | 3篇 |
2022年 | 27篇 |
2021年 | 31篇 |
2020年 | 25篇 |
2019年 | 14篇 |
2018年 | 37篇 |
2017年 | 43篇 |
2016年 | 60篇 |
2015年 | 72篇 |
2014年 | 69篇 |
2013年 | 96篇 |
2012年 | 68篇 |
2011年 | 78篇 |
2010年 | 45篇 |
2009年 | 37篇 |
2008年 | 36篇 |
2007年 | 28篇 |
2006年 | 27篇 |
2005年 | 30篇 |
2004年 | 18篇 |
2003年 | 15篇 |
2002年 | 15篇 |
2001年 | 4篇 |
2000年 | 10篇 |
1999年 | 12篇 |
1998年 | 9篇 |
1997年 | 5篇 |
1996年 | 9篇 |
1995年 | 3篇 |
1994年 | 8篇 |
1993年 | 3篇 |
1992年 | 7篇 |
1991年 | 5篇 |
1990年 | 7篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1978年 | 2篇 |
1976年 | 4篇 |
1972年 | 4篇 |
1968年 | 2篇 |
1965年 | 1篇 |
1939年 | 1篇 |
1934年 | 1篇 |
排序方式: 共有1006条查询结果,搜索用时 15 毫秒
211.
212.
Jameel R. Al-Obaidi Noor Baity Saidi Siti Rokhiyah Ahmad Usuldin Siti Nahdatul Isnaini Said Hussin Noornabeela Md Yusoff Abu Seman Idris 《The protein journal》2016,35(2):100-106
Ganoderma species are a group of fungi that have the ability to degrade lignin polymers and cause severe diseases such as stem and root rot and can infect economically important plants and perennial crops such as oil palm, especially in tropical countries such as Malaysia. Unfortunately, very little is known about the complex interplay between oil palm and Ganoderma in the pathogenesis of the diseases. Proteomic technologies are simple yet powerful tools in comparing protein profile and have been widely used to study plant–fungus interaction. A critical step to perform a good proteome research is to establish a method that gives the best quality and a wide coverage of total proteins. Despite the availability of various protein extraction protocols from pathogenic fungi in the literature, no single extraction method was found suitable for all types of pathogenic fungi. To develop an optimized protein extraction protocol for 2-DE gel analysis of Ganoderma spp., three previously reported protein extraction protocols were compared: trichloroacetic acid, sucrose and phenol/ammonium acetate in methanol. The third method was found to give the most reproducible gels and highest protein concentration. Using the later method, a total of 10 protein spots (5 from each species) were successfully identified. Hence, the results from this study propose phenol/ammonium acetate in methanol as the most effective protein extraction method for 2-DE proteomic studies of Ganoderma spp. 相似文献
213.
214.
Thirty-four monosporous isolates ofH. gramincum were grown on potato-dextrose-agar medium and their cultural characters and rate of growth were studied. None of the isolates sporulated in culture. Conidiophores and conidia from natural host were measured. On the basis of cultural and morphological characters these isolates were distinguished as seven distinct races. 相似文献
215.
Thirty-four monosporous isolates ofH. gramineum
Rab., the incitant of leaf stripe of barley, were divided in seven categories on the basis of morphological and cultural characters. Seventy-three Indian and American varieties of barley were tested for their reaction against the seven isolates. Detailed observations on prevalence and severity have been taken and a new method has been developed for calculating infection value in each case. There are distinct differences among the isolates in their prevalence and severity on the barley varieties and thus these isolates are distinct races. 相似文献
216.
M. Basri A. B. Salleh K. Ampon W. M. Z. Yunus C. N. A. Razak 《Biocatalysis and Biotransformation》1991,4(4):313-317
Lipase was modified using polyethylene glycol activated by p-nitrochloroformate. The hydrolytic activity of the polyethylene glycol-derivatised lipase (PEG-lipase) was relatively low compared with that of the unmodified enzyme in aqueous system. The esterification activity, however, was enhanced following the modification. The rate of esterification of butyric acid was higher than that of oleic acid. Benzene was the best solvent for the esterification reaction. 相似文献
217.
M Ali C J Gubler J Al Saleh F Abu Farsak 《Comparative biochemistry and physiology. B, Comparative biochemistry》1987,87(4):833-835
1. A study was made of transketolase activity in red and white blood cells and of conditions for assay for transketolase activity and for assessment of the "TPP effect" in human and rat blood. 2. The ratio of the transketolase activity in white cells to that in red cells varied between 23 and 93. 3. Red cells or white cells can both be used for assessment of transketolase activity and the "TPP effect", but the best source for evaluation of transketolase activity and the percent change on addition of thiamin diphosphate appears to be whole blood. 相似文献
218.
Continuous production of the rotifer Brachionus plicatilis rotundiformis (S-type) in an intensive chemostat culture system has been investigated. The production dynamics of rotifers in relation to different flow rates and feed regimes show that the growth rate and production depends on the type of algal feed and flow rate utilized in the culture system. It was possible to achieve a mean production of up to 318.84 × 106 rotifers m–3 d–1 at a flow rate of 6 1 h–1 in 100 1 chemostats and up to 261.21 × 106 rotifers m–3 d–1 at a flow rate of 40 1 h –1 while using 1 m3 capacity rotifer chemostats as production units. The 3 fatty acid composition of rotifers while using Chlorella and Nannochloropsis in the culture system has been described. The results of this investigation show that the rotifer productivity in the continuous culture system is considerably higher than in any of the conventional culture systems described to date for aquacultural purposes.This research was financed by the Kuwait Foundation for the Advancement of Sciences (KFAS), Kuwait, under a contract research project code 86-04-02. 相似文献
219.
We isolated the unialgal strain of Cylindotheca closterium (Ehrenb.) Reimann et J. C. Lewin and produced an axenic strain using an antibiotic cocktail of enriched f/2 artificial seawater medium. The optimal growth conditions were estimated under 27 different combinations of temperature, salinity, and nutrients, and mass culture was performed based on the best specific growth conditions. Its antioxidant activities were determined from the extracts of methanol, water, and enzymes (proteases and carbohydrases). The maximum specific growth rate (μmax) varied from 0.63 to 0.97 · d?1. The maximum cell density was 7.20 × 104 cells · mL?1, while the μmax was 0.82 · d?1 in culture conditions of 20°C, 30 psu (practical salinity unit), and “F” nutrient concentrations on day 10 of the culture period. The scavenging rates for 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) free radical were 72.5% and 69.4% from Viscozyme and methanol extracts, respectively. The enzymatic extracts of C. closterium prepared by the hydrolyses of Amyloglucosidase (AMG) and Viscozyme showed 45.8% and 45.5% nitric‐oxide‐scavenging rates, slightly lower than the activity of alpha‐tocopherol (α‐tocopherol) but similar to butylated hydroxytoluene (BHT). The extract from methanol and water showed 44.8% and 44.4% scavenging rates, statistically similar with BHT. The metal‐chelating activities of the Kojizyme, Alcalase, methanol, Viscozyme, and Neutrase extracts were 67.1, 53.9, 53.2, 52.1, and 50.2 %, respectively, five to six times higher than the commercial antioxidants. The AMG, Viscozyme, and Neutrase extracts showed a remarkable linoleic acid peroxidation inhibition, which was higher than BHT and statistically similar with α‐tocopherol. 相似文献
220.
Nidal Abu Laban Draženka Selesi Thomas Rattei Patrick Tischler Rainer U. Meckenstock 《Environmental microbiology》2010,12(10):2783-2796
Anaerobic benzene degradation was studied with a highly enriched iron‐reducing culture (BF) composed of mainly Peptococcaceae‐related Gram‐positive microorganisms. The proteomes of benzene‐, phenol‐ and benzoate‐grown cells of culture BF were compared by SDS‐PAGE. A specific benzene‐expressed protein band of 60 kDa, which could not be observed during growth on phenol or benzoate, was subjected to N‐terminal sequence analysis. The first 31 amino acids revealed that the protein was encoded by ORF 138 in the shotgun sequenced metagenome of culture BF. ORF 138 showed 43% sequence identity to phenylphosphate carboxylase subunit PpcA of Aromatoleum aromaticum strain EbN1. A LC/ESI‐MS/MS‐based shotgun proteomic analysis revealed other specifically benzene‐expressed proteins with encoding genes located adjacent to ORF 138 on the metagenome. The protein products of ORF 137, ORF 139 and ORF 140 showed sequence identities of 37% to phenylphosphate carboxylase PpcD of A. aromaticum strain EbN1, 56% to benzoate‐CoA ligase (BamY) of Geobacter metallireducens and 67% to 3‐octaprenyl‐4‐hydroxybenzoate carboxy‐lyase (UbiD/UbiX) of A. aromaticum strain EbN1 respectively. These genes are proposed as constituents of a putative benzene degradation gene cluster (~17 kb) composed of carboxylase‐related genes. The identified gene sequences suggest that the initial activation reaction in anaerobic benzene degradation is probably a direct carboxylation of benzene to benzoate catalysed by putative anaerobic benzene carboxylase (Abc). The putative Abc probably consists of several subunits, two of which are encoded by ORFs 137 and 138, and belongs to a family of carboxylases including phenylphosphate carboxylase (Ppc) and 3‐octaprenyl‐4‐hydroxybenzoate carboxy‐lyase (UbiD/UbiX). 相似文献