全文获取类型
收费全文 | 320篇 |
免费 | 22篇 |
出版年
2020年 | 3篇 |
2016年 | 5篇 |
2015年 | 9篇 |
2014年 | 15篇 |
2013年 | 7篇 |
2012年 | 17篇 |
2011年 | 8篇 |
2010年 | 17篇 |
2009年 | 16篇 |
2008年 | 9篇 |
2007年 | 8篇 |
2006年 | 7篇 |
2005年 | 12篇 |
2004年 | 5篇 |
2003年 | 12篇 |
2002年 | 7篇 |
2001年 | 9篇 |
2000年 | 7篇 |
1999年 | 9篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 8篇 |
1992年 | 7篇 |
1991年 | 5篇 |
1990年 | 8篇 |
1989年 | 8篇 |
1988年 | 15篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1984年 | 7篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1979年 | 2篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1974年 | 3篇 |
1973年 | 5篇 |
1972年 | 3篇 |
1971年 | 4篇 |
1969年 | 4篇 |
1968年 | 2篇 |
1967年 | 4篇 |
1963年 | 2篇 |
1962年 | 2篇 |
1936年 | 2篇 |
1932年 | 3篇 |
1931年 | 2篇 |
1929年 | 4篇 |
排序方式: 共有342条查询结果,搜索用时 15 毫秒
261.
Half-bushel parcels of wheat at 15 and 19% moisture content (MC) were implanted in bulks of dry stored oats in a farm granary for 60 weeks to monitor quality changes. Temperature, MC, fat acidity value (FAV), oxygen and carbon dioxide levels, germination, microfloral incidence and abundance, and major storage mycotoxins (aflatoxins, sterigmatocystin, ochratoxin A, citrinin, penicillic acid) were monitored at two-week or four-week intervals during storage. Wheat storage at 19% MC was characterized by higher temperature, MC, FAV, carbon dioxide level, and microfloral abundance, and lower levels of oxygen and germination as compared to 15% MC wheat; no mycotoxins were detected at either moisture. Approximately 19% MC may represent a critical moisture limit for mycotoxin formation in granary stored wheat, given the indigenous mycoflora. 相似文献
262.
Ryanodine stabilizes multiple conformational states of the skeletal muscle calcium release channel. 总被引:12,自引:0,他引:12
E Buck I Zimanyi J J Abramson I N Pessah 《The Journal of biological chemistry》1992,267(33):23560-23567
Nanomolar to micromolar ryanodine alters the gating kinetics of the Ca2+ release channel from skeletal sarcoplasmic reticulum (SR) fused with bilayer lipid membranes (BLM). In the presence of asymmetric CsCl and 100 microM CaCl2 cis, ryanodine (RY) (5-40 nM) activates the channel, increasing the open probability (po; maximum 300% of control) without changing unitary conductance (468 picosiemens (pS)). Statistical analyses of gating kinetics reveal that open and closed dwell times exhibit biexponential distributions and are significantly modified by nanomolar RY. Altered channel gating kinetics with low nanomolar RY is fully reversible and correlates well with binding kinetics of nanomolar [3H]RY with its high affinity site (Kd1 = 0.7 nM) under identical experimental conditions. RY (20-50 nM) induces occasional 1/2 conductance fluctuations which correlate with [3H]RY binding to a second site having lower affinity (Kd2 = 23 nM). RY (5-50 nM) in the presence of 500 mM CsCl significantly enhances Ca(2+)-induced Ca2+ release from actively loaded SR vesicles. Ryanodine > or = 50 nM stabilizes the channel in a 234-pS subconductance which is not readily reversible. RY (> or = 70 microM) produces a unidirectional transition from the 1/2 to a 1/4 conductance fluctuation, whereas RY > or = 200 microM causes complete closure of the channel. The RY required for stabilizing 1/4 conductance transitions and channel closure do not quantitatively correlate with [3H]RY equilibrium binding constants and is attributed to significant reduction in association kinetics with > 200 nM [3H]RY in the presence of 500 mM CsCl. These results demonstrate that RY stabilizes four discrete states of the SR release channel and supports the existence of multiple interacting RY effector sites on the channel protein. 相似文献
263.
264.
Reconstitution of hepatic uricase in planar lipid bilayer reveals a functional organic anion channel
E. Leal-Pinto R. D. London B. A. Knorr R. G. Abramson 《The Journal of membrane biology》1995,146(2):123-132
Rat renal proximal tubule cell membranes have been reported to contain uricase-like proteins that function as electrogenic urate transporters. Although uricase, per se, has only been detected within peroxisomes in rat liver (where it functions as an oxidative enzyme) this protein has been shown to function as a urate transport protein when inserted into liposomes. Since both the uricase-like renal protein and hepatic uricase can transport urate, reconstitution studies were performed to further characterize the mechanism by which uricase may function as a transport protein. Ion channel activity was evaluated in planar lipid bilayers before and after fusion of uricase-containing proteoliposomes. In the presence of symmetrical solutions of urate and KCl, but absence of uricase, no current was generated when the voltage was ramped between ±100 mV. Following fusion of uricase with the bilayer, single channel activity was evident: the reconstituted channel rectified with a mean slope conductance of 8 pS, displayed voltage sensitivity, and demonstrated a marked selectivity for urate relative to K+ and Cl–. The channel was more selective to oxonate, an inhibitor of both enzymatic uricase activity and urate transport, than urate and it was equally selective to urate and pyrazinoate, an inhibitor of urate transport. With time, pyrazinoate blocked both its own movement and the movement of urate through the channel. Channel activity was also blocked by the IgG fraction of a polyclonal antibody to affinity purified pig liver uricase. These studies demonstrate that a highly selective, voltage dependent organic anion channel is formed when a purified preparation of uricase is reconstituted in lipid bilayers.This work was supported in part by the G. Harold and Leila Y. Mathers Charitable Foundation (E.L.P. and R.D.L.), the Irma T. Hirschl Trust (R.D.L.), National Institutes of Health grant DK08419 (B.A.K.) and a Grant-in-Aid from the American Heart Association, N.Y.C. Affiliate (R.G.A.). 相似文献
265.
The isolation, identification and synthesis of two metabolites of guanethidine formed in pig and rabbit liver homogenates 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
1. Two metabolites of radioactively labelled guanethidine were isolated from rabbit and pig liver homogenates by ion-exchange chromatography on a sulphonic acid resin. 2. One of the metabolites was eluted from the column with ammonia and identified as 2-(6-carboxyhexylamino)ethylguanidine on the basis of the elemental analysis, i.r. spectrum and pH titration curve of the pure compound, and the observed partial loss of tritium for ring-labelled guanethidine during the formation of this metabolite. 3. This identification was confirmed by synthesis. 4. 2-(6-Carboxyhexylamino)ethylguanidine underwent ring-closure in hot alkaline solution to 1-(6-carboxyhexyl)-2-iminoimidazolidine. 5. The other metabolite of guanethidine was eluted from the ion-exchange column with 6m-hydrochloric acid along with the unchanged drug. It was purified by countercurrent distribution and shown to be identical with synthetic guanethidine N-oxide. 6. The two metabolites and the product of ring-closure had less than one-tenth of the antihypertensive activity of guanethidine in the renal-hypertensive rat and are unlikely to contribute to the pharmacological properties of the drug. 相似文献
266.
Two major alkaloids from Buxus sempervirens, cyclovirobuxine-D and cyclobuxine-D, were found to be radioactively labelled following administration of mevalonic acid [2-14C,(4R)-4-3H1] to freshly-harvested shoots. The 3H: 14C atomic ratio of 3:4 in cyclovirobuxine-D indicated a biosynthetic pathway from cycloartenol involving 3-ketone and 20-ketone intermediates. A 3H: 14C atomic ratio of ca 3:3 in cyclobuxine-D suggests that the 4α-methyl group of cycloartenol is lost in its formation, and this conforms with current theories of the sequence of C-4 demethylation of sterols. 相似文献
267.
268.
A specific collagenase (EC 3.4.24.3) has been found and purified from serum-free culture medium of 11095 epidermoid carcinoma of rat prostate. The molecular weight of this collagenase was estimated at 71 000 and the pH optimum was approx. 7. At 26 degrees C, the collagenase cleaved collagen at a site 3/4 the length from the N-terminus. At 37 degrees C, this collagenase degraded collagen to smaller peptides. The enzyme activity was inhibited by serum, cysteine and EDTA, but not by protease inhibitors. The presence of collagenase in rat tumor tissue suggests that this enzyme might play a significant role in tissue invasion by cancer cells. 相似文献
269.
KIF3C and KIF3A Form a Novel Neuronal Heteromeric Kinesin That Associates with Membrane Vesicles 总被引:8,自引:2,他引:6
下载免费PDF全文
![点击此处可从《Molecular biology of the cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Virgil Muresan Tatiana Abramson Asya Lyass Dirk Winter Elena Porro Filbert Hong Nancy L. Chamberlin Bruce J. Schnapp 《Molecular biology of the cell》1998,9(3):637-652
We have cloned from rat brain the cDNA encoding an 89,828-Da kinesin-related polypeptide KIF3C that is enriched in brain, retina, and lung. Immunocytochemistry of hippocampal neurons in culture shows that KIF3C is localized to cell bodies, dendrites, and, in lesser amounts, to axons. In subcellular fractionation experiments, KIF3C cofractionates with a distinct population of membrane vesicles. Native KIF3C binds to microtubules in a kinesin-like, nucleotide-dependent manner. KIF3C is most similar to mouse KIF3B and KIF3A, two closely related kinesins that are normally present as a heteromer. In sucrose density gradients, KIF3C sediments at two distinct densities, suggesting that it may be part of two different multimolecular complexes. Immunoprecipitation experiments show that KIF3C is in part associated with KIF3A, but not with KIF3B. Unlike KIF3B, a significant portion of KIF3C is not associated with KIF3A. Consistent with these biochemical properties, the distribution of KIF3C in the CNS has both similarities and differences compared with KIF3A and KIF3B. These results suggest that KIF3C is a vesicle-associated motor that functions both independently and in association with KIF3A. 相似文献
270.
Recombinant protein, designated UAT, prepared from a cloned rat renal cDNA library functions as a selective voltage-sensitive
urate transporter/channel when fused with lipid bilayers. Since we previously suggested that UAT may represent the mammalian
electrogenic urate transporter, UAT has been functionally characterized in the presence and absence of potential channel blockers,
several of which are known to block mammalian electrogenic urate transport. Two substrates, oxonate (a competitive uricase
inhibitor) and pyrazinoate, that inhibit renal electrogenic urate transport also block UAT activity. Of note, oxonate selectively
blocks from the cytoplasmic side of the channel while pyrazinoate only blocks from the channel's extracellular face. Like
oxonate, anti-uricase (an electrogenic transport inhibitor) also selectively blocks channel activity from the cytoplasmic
side. Adenosine blocks from the extracellular side exclusively while xanthine blocks from both sides. These effects are consistent
with newly identified regions of homology to uricase and the adenosine A1/A3 receptor in UAT and localize these homologous
regions to the cytoplasmic and extracellular faces of UAT, respectively. Additionally, computer analyses identified four putative
α-helical transmembrane domains, two β sheets, and blocks of homology to the E and B loops of aquaporin-1 within UAT. The experimental observations substantiate our proposal that UAT is the molecular representation
of the renal electrogenic urate transporter and, in conjunction with computer algorithms, suggest a possible molecular structure
for this unique channel.
Received: 13 October 1998/Revised: 28 January 1999 相似文献