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941.
Congenital atrichia is a rare autosomal recessive disorder of hair development, characterized by complete loss of hair shortly
after birth. Evidence of linkage to chromosome 8p12 has been established, implicating the human homolog of the mouse hairless
(hr) gene as a candidate gene. We have previously identified missense mutations in families with congenital atrichia. Here,
we report the first deletion mutation (2147del C) in exon 9 of the human hairless gene leading to a frameshift and downstream
premature termination codon in five Palestinian families of Arab origin.
Received: 31 July 1998 / Accepted: 31 August 1998 相似文献
942.
Treatment of Dutch iris (Iris × hollandica Hoog. cv. Sapphire Beauty) bulbs with ethylene prior to precooling stimulated flowering in bulbs of various sizes. In large sized bulbs exposure to ethylene followed by precooling resulted in 100% flowering over a five months period after planting. Flowering in control bulbs which were not treated with ethylene prior to precooling was limited to 67% during the same five months period. In medium sized bulbs flowering in the ethylene treatment was 90% compared to 75% in the control. However, the biggest stimulation of flowering by ethylene was found in small sized bulbs (from 16 to 56%). Application of octanoic acid for a short time period prior to exposure to ethylene stimulated flowering in all bulb sizes. After five months the final percentage flowering in large and medium sized bulbs of the octanoic acid plus ethylene treatment did not differ from that of the ethylene only treatment. However, the initial rate of flowering was higher in the former treatment. In small bulbs the percentage flowering was much higher in the octanoic acid plus ethylene treatment than in the ethylene only treatment. The results of this study indicate that, just as in certain flowers, fruit and seeds, treatment with octanoic acid stimulates ethylene sensitivity in Dutch iris bulbs. The sensitivity of untreated bulbs to ethylene was highest in large bulbs and lowest in small bulbs. This correlated well with the endogenous octanoic acid content of the bulbs. Octanoic acid levels were highest in large bulbs and lowest in small Bulbs. It appears that the endogenous levels of octanoic acid in the bulbs is determined prior to the onset of dormancy. 相似文献
943.
944.
Orly Ardon Raphael Nudelman Catherine Caris Jacqueline Libman Abraham Shanzer Yona Chen Yitzhak Hadar 《Journal of bacteriology》1998,180(8):2021-2026
In this study, we monitored and compared the uptake of iron in the fungus Ustilago maydis by using biomimetic siderophore analogs of ferrichrome, the fungal native siderophore, and ferrioxamine B (FOB), a xenosiderophore. Ferrichrome-iron was taken up at a higher rate than FOB-iron. Unlike ferrichrome-mediated uptake, FOB-mediated iron transport involved an extracellular reduction mechanism. By using fluorescently labeled siderophore analogs, we monitored the time course, as well as the localization, of iron uptake processes within the fungal cells. A fluorescently labeled ferrichrome analog, B9-lissamine rhodamine B, which does not exhibit fluorescence quenching upon iron binding, was used to monitor the entry of the compounds into the fungal cells. The fluorescence was found intracellularly 4 h after the application and later was found concentrated in two to three vesicles within each cell. The fluorescence of the fluorescently labeled FOB analog CAT18, which is quenched by iron, was visualized around the cell membrane after 4 h of incubation with the ferrated (nonfluorescent) compounds. This fluorescence intensity increased with time, demonstrating fungal iron uptake from the siderophores, which remained extracellular. We here introduce the use of fluorescent biomimetic siderophores as tools to directly track and discriminate between different pathways of iron uptake in cells. 相似文献
945.
Martinez-Salas Jose; Mendelssohn Richard; Abraham William M.; Hsiao Bernard; Ahmed Tahir 《Journal of applied physiology》1998,84(1):222-228
Martinez-Salas, José, Richard Mendelssohn, William M. Abraham, Bernard Hsiao, and Tahir Ahmed. Inhibition of allergic airway responses by inhaled low-molecular-weight heparins:molecular-weight dependence. J. Appl.Physiol. 84(1): 222-228, 1998.Inhaled heparin prevents antigen-induced bronchoconstriction and inhibitsanti-immunoglobulin E-mediated mast cell degranulation. We hypothesizedthat the antiallergic action of heparin may be molecular weightdependent. Therefore, we studied the effects of three differentlow-molecular-weight fractions of heparin [medium-, low-, andultralow-molecular-weight heparin (MMWH, LMWH, ULMWH,respectively)] on the antigen-induced acute bronchoconstrictorresponse (ABR) and airway hyperresponsiveness (AHR) in allergic sheep.Specific lung resistance was measured in 22 sheep before and afterairway challenge with Ascarissuum antigen, without and afterpretreatment with inhaled fractionated heparins at doses of0.31-5.0 mg/kg. Airway responsiveness was estimated before and 2 hpostantigen as the cumulative provocating dose of carbachol in breathunits that increased specific lung resistance by 400%. Allfractionated heparins caused a dose-dependent inhibition of ABR andAHR. ULMWH was the most effective fraction, with the inhibitory dosecausing 50% protection (ID50)against ABR of 0.5 mg/kg, whereasID50 values of LMWH and MMWH were1.25 and 1.8 mg/kg, respectively. ULMWH was also the most effective fraction in attenuating AHR; theID50 values for ULMWH, LMWH, andMMWH were 0.5, 2.5, and 4.7 mg/kg, respectively. These data suggestthat 1) fractionatedlow-molecular-weight heparins attenuate antigen-induced ABR and AHR;2) there is an inverse relationship between the antiallergic activity of heparin fractions and molecular weight; and 3) ULMWH is the mosteffective fraction preventing allergic bronchoconstriction and airwayhyperresponsiveness. 相似文献
946.
Role of tachykinins in airway responses to ozone in rats 总被引:1,自引:0,他引:1
Takebayashi Toru; Abraham Joseph; Murthy G. G.Krishna; Lilly Craig; Rodger Ian; Shore Stephanie A. 《Journal of applied physiology》1998,85(2):442-450
Previousstudies that used neonatal capsaicin (Cap) treatment toablate C fibers indicate that C fibers act to inhibit lung damage andairway hyperresponsiveness after ozone(O3) exposure in rats. Thepurpose of this study was to determine1) the role of tachykinins in theseprotective effects and 2) whetherdifferences in minute ventilation (E)during O3 exposure might accountfor the effect of Cap. In the first study, male Sprague-Dawley rats were exposed to 1 part/million O3or air for 3 h. Four hours later, a bronchoalveolar lavage (BAL) wasperformed or airway responsiveness was measured. Rats were treated withCP-99994 and SR-48968, selective neurokinin-1- and -2-receptorantagonists, respectively, or with vehicle (Veh).O3 caused an increase in thenumber of neutrophils recovered from BAL fluid in both the Veh-treatedand tachykinin-receptor antagonist (TKRA)-treated rats, but the numberof neutrophils was approximately twofold greater in the TKRA-treatedrats. In contrast, TKRA treatment had no effect on baseline pulmonarymechanics or airway responsiveness. AfterO3 exposure, the number ofneutrophils in BAL fluid was also greater in Cap- than in Veh-treatedrats. O3 reducedE in both Veh- and Cap-treated rats,but the response was greater (reduction of 44.7 ± 3.7 vs. 27.8 ± 6.8%) and occurred earlier (10 vs. 70 min) in Cap- than inVeh-treated rats (P < 0.02). Theseresults suggest that tachykinins mediate protective effects of C fibersagainst O3-induced lunginflammation. The results also indicate that the more pronounced effectof O3 on BAL neutrophils inCap-treated rats is not the result of a greater inhaled dose ofO3 resulting from greaterE. 相似文献
947.
F. C. Steward and Jakob Reinert in the late 1950s, independently and with different degrees of scientific exactness, demonstrated that somatic cells of cultivated carrot can produce embryo-like structures in aseptic culture. Growth substances in the nutrient medium were viewed as central to the process. The now classic papers of Steward and Reinert have found a special and enduring place in the literature of plant development. But Harry Waris also deserves credit for his observation that vegetative cells sloughed off from aseptically germinated seedlings reared in liquid nutrient medium can produce 'embryos'. In his studies, seedlings of Oenanthe aquatica (Umbelliferae) were maintained in culture for protracted periods under nutrient conditions designed to foster imbalance in protein metabolism, but without exogenous growth hormones. Seedlings placed in media with high concentrations of glycine grew normally for 3–4 months; after this a "period of morbidity" occurred, followed by production of new plants from the root tips. These new plants, later called "neomorphs", in turn reproduced by colorless outgrowths of leaf epidermis. Such outgrowths, and "nodules" formed in a callus produced by the original seedlings, passed through stages described as "nodule", "fusiform", and a stage with two or more "lobes". Transfer of the neomorphs to a medium lacking glycine resulted in the development of normal plants. We show that Waris was among the first, if not the first, to observe and recognize somatic embryo production in aseptic culture, and indeed to call them "embryos". We also discuss his investigations in the context of understanding development at the cellular level, then and now. 相似文献
948.
949.
Launois Sandrine H.; Averill Nathan; Abraham Joseph H.; Kirby Debra A.; Weiss J. Woodrow 《Journal of applied physiology》2001,90(1):114-120
Spontaneous and provoked nonrespiratoryarousals can be accompanied by a patterned hemodynamic response. Toinvestigate whether a patterned response is also elicited byrespiratory arousals, we compared nonrespiratory arousals (NRA) torespiratory arousals (RA) induced by airway occlusion during non-rapideye movement sleep. We monitored mean arterial blood pressure (MAP),heart rate, iliac and renal blood flow, and sleep stage in 7 pigsduring natural sleep. Iliac and renal vascular resistance werecalculated. Airway occlusions were obtained by manually inflating achronically implanted tracheal balloon during sleep. The balloon wasquickly deflated as soon as electroencephalogram arousal occurred. As previously reported, NRA generally elicited iliac vasodilation, renalvasoconstriction, little change in MAP, and tachycardia. In contrast,RA generally elicited iliac and renal vasoconstriction, an increase inMAP and tachycardia. The frequent occurrence of iliac vasoconstrictionand arterial pressure elevation following RA but not NRA suggests thatsleep state change alone does not account for the hemodynamic responseto airway occlusion during sleep. 相似文献
950.