全文获取类型
收费全文 | 114篇 |
免费 | 17篇 |
专业分类
131篇 |
出版年
2022年 | 1篇 |
2021年 | 3篇 |
2019年 | 2篇 |
2017年 | 2篇 |
2016年 | 3篇 |
2015年 | 2篇 |
2014年 | 2篇 |
2013年 | 5篇 |
2012年 | 4篇 |
2011年 | 5篇 |
2010年 | 5篇 |
2009年 | 2篇 |
2008年 | 6篇 |
2007年 | 4篇 |
2006年 | 2篇 |
2005年 | 7篇 |
2004年 | 6篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 5篇 |
2000年 | 6篇 |
1999年 | 5篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 5篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1985年 | 4篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有131条查询结果,搜索用时 0 毫秒
31.
Oren E. Livne Lide Han Gorka Alkorta-Aranburu William Wentworth-Sheilds Mark Abney Carole Ober Dan L. Nicolae 《PLoS computational biology》2015,11(3)
Founder populations and large pedigrees offer many well-known advantages for genetic mapping studies, including cost-efficient study designs. Here, we describe PRIMAL (PedigRee IMputation ALgorithm), a fast and accurate pedigree-based phasing and imputation algorithm for founder populations. PRIMAL incorporates both existing and original ideas, such as a novel indexing strategy of Identity-By-Descent (IBD) segments based on clique graphs. We were able to impute the genomes of 1,317 South Dakota Hutterites, who had genome-wide genotypes for ~300,000 common single nucleotide variants (SNVs), from 98 whole genome sequences. Using a combination of pedigree-based and LD-based imputation, we were able to assign 87% of genotypes with >99% accuracy over the full range of allele frequencies. Using the IBD cliques we were also able to infer the parental origin of 83% of alleles, and genotypes of deceased recent ancestors for whom no genotype information was available. This imputed data set will enable us to better study the relative contribution of rare and common variants on human phenotypes, as well as parental origin effect of disease risk alleles in >1,000 individuals at minimal cost. 相似文献
32.
Jo?o?Luiz?S?Moreira Rodrigo?M?Mota Maria?F?Horta Santuza?MR?Teixeira Elisabeth?Neumann Jacques?R?Nicoli álvaro?C?NunesEmail author 《BMC microbiology》2005,5(1):15
Background
The accurate identification of Lactobacillus and other co-isolated bacteria during microbial ecological studies of ecosystems such as the human or animal intestinal tracts and food products is a hard task by phenotypic methods requiring additional tests such as protein and/or lipids profiling. 相似文献33.
34.
JR Abney CD Meliza B Cutler M Kingma JE Lochner BA Scalettar 《Biophysical journal》1999,77(5):2887-2895
Secretory granules containing a hybrid protein consisting of the regulated secretory protein tissue plasminogen activator and an enhanced form of green fluorescent protein were tracked at high spatial resolution in growth cones of differentiated PC12 cells. Tracking shows that granules, unlike synaptic vesicles, generally are mobile in growth cones. Quantitative analysis of trajectories generated by granules revealed two dominant modes of motion: diffusive and directed. Diffusive motion was observed primarily in central and peripheral parts of growth cones, where most granules diffused two to four orders of magnitude more slowly than comparably sized spheres in dilute solution. Directed motion was observed primarily in proximal parts of growth cones, where a subset of granules underwent rapid, directed motion at average speeds comparable to those observed for granules in neurites. This high-resolution view of the dynamics of secretory granules in growth cones provides insight into granule organization and release at nerve terminals. In particular, the mobility of granules suggests that granules, unlike synaptic vesicles, are not tethered stably to cytoskeletal structures in nerve terminals. Moreover, the slow diffusive nature of this mobility suggests that secretory responses involving centrally distributed granules in growth cones will occur slowly, on a time scale of minutes or longer. 相似文献
35.
Sianos G Kay IP Carlier SG Lighart JM Wardeh AJ Coen VL Levendag PC Serruys PW 《International journal of cardiovascular interventions》2000,3(2):121-125
The application of beta-radiation in coronary arteries is a promising new technique for the treatment of in-stent restenosis. This is the first case in which the 5 F. delivery catheter of the Beta-Cath trade mark system was advanced through the struts of a stent, previously deployed in an adjacent branch, so as to deliver radiation to the target vessel. 相似文献
36.
A review of the fungi associated with soybean seeds, pods, or flowers was conducted in North America. Species of Deuteromycetes
are the most common fungi in each of the soybean flower organs followed by the Ascomycetes and Phycomycetes which comprise
about one-fourth of the total mycoflora. Eighty genera and about 135 or more species occur in seeds, pods, or flowers. With
regard to numbers of taxa from separate mycofloras, 63 genera and about 108 or more species occur in seeds, 65 genera and
about 88 or more species occur in pods, and 36 genera and approximately 47 or more species occur in flowers. Most of the fungi
which occur in flowers can be cultured from pods, and the majority of those fungi occur in seeds. Methods for and a key are
provided for the identification of the 30 most important selected fungi.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
37.
We studied seasonal and spatial variability in the reproductive life-history traits of Caribbean gambusia, Gambusia puncticulata puncticulata, using collections representing dry and wet periods from eight pond sites located across the three Cayman Islands. Caribbean gambusia exhibited a seasonal life-history response over the 5-month interval between the relatively dry and wet periods, marked by shifts to larger adult sizes and smaller broods made up of larger offspring. This seasonal shift in the life-history pattern coincided with increased rainfall, lower salinity, lower water temperature, and higher food availability. Overall, there was a reproductive trade-off involving a reciprocal relationship between brood size and mean embryo mass, and a direct relationship between brood size and total embryo mass. Levels of various environmental variables, including salinity, submerged aquatic vegetation cover, and capture depth, were apparently unrelated to the life-history pattern. Furthermore, the life-history pattern did not reflect an island effect. However, a correlation between the seasonal difference in salinity and offspring size suggested that the Cayman Island life-history pattern may correspond in part with the environmental stability hypothesis. 相似文献
38.
E R Abney 《Biochemical Society transactions》1985,13(2):422-424
39.
The aim of this study is determining the different patterns of egg nests and the morphological differences between the specimens of Cicadatra persica Kirkalidy, 1909 (Hemiptera: Cicadidae) distributed in fruit orchards in Erneh located on AL-Sheikh mountain south west of Syria. The appearance of 80 egg nests was studied, and the results showed that there were two basic patterns of egg nests laid by Cicadatra persica, 90% of the egg nests were of the first pattern (consists of several adjacent slits), while 10% of them were of the second pattern (consists of several divergent slits). A random sample consisting of 300 specimens (150 males and 150 females) were also studied concentrating on the differences in the color of the supra-antennal plate and in the number of spurs on the tibia of the hind legs. The results showed that there were two basic patterns of individuals based on the differences in the color of supra-antennal plate. The first pattern (individuals with yellow supra-antennal plates), constituted more than 90%, and the second one (individuals with black supra-antennal plates) constituted less than 10%. The results also showed that there were 27 different patterns based on the number of spurs on the tibia of the hind legs. One of them was a common pattern (2, 3) whose individuals have 2 spurs on the upper side of the tibia of the hind legs and 3 spurs on the lateral side of the tibia of the hind legs. The total percent of this common pattern was 76%. The other 26 patterns were different from each other, and the total percent of all these different patterns was 24%. 相似文献
40.
MR D'Andrea 《Biotechnic & histochemistry》2013,88(2):55-64
Reliable immunohistochemical detection of collagen in formalin fixed, paraffin embedded tissues requires protease digestion. While these pan-proteases (pepsin, trypsin, protease K, etc.) enhance collagen detection, they also digest many other tissue proteins and produce poor cellular morphology and unrecognizable cellular structures. Balancing the conditions (protease type, concentration, incubation time and temperature) to digest some, but not all, proteins in a tissue section while optimizing collagen detection requires one to compromise improved collagen immunolabeling with adequate cellular morphology. Furthermore, optimal conditions for digesting tissue proteins to enhance collagen detection vary among tissue types and their fixation. Although brain is not typically subject to these deleterious consequences, structures such as epithelium, spermatids, stroma etc. and other tissues with complicated histology are profoundly affected. To resolve this technical dilemma, we discovered a novel use for collagenase to enhance collagen immunodetection without affecting the noncollagen proteins, thereby preserving tissue morphology. Collagenase, which is typically used in vitro for disassociation of cells, has never been used reliably on formalin fixed, paraffin embedded tissue sections. This new use of collagenase for immunohistochemistry promotes increased collagen immunolabeling, is easy to use, is versatile, and allows preservation of tissue structure that provides maximal and accurate histological information. 相似文献