排序方式: 共有51条查询结果,搜索用时 234 毫秒
21.
Ferjani Zouidi Mouna Stayoussef Dorra Bouzid Hajer Fourati Olfa Abida Costa João Mourad Ben Ayed Raouia Fakhfakh Kammoun Thouraya Hachicha Monjia Penha-Gonçalves Carlos Hatem Masmoudi 《Gene》2014
Type 1 diabetes (T1D) is an autoimmune disease (AID) with both genetic and environmental components. We aimed to investigate the genetic association of polymorphisms in genes previously linked with other AIDs, namely BANK1, IL15 and IL2/IL21 region. 相似文献
22.
Exogenously applied 5-aminolevulinic acid modulates growth,secondary metabolism and oxidative defense in sunflower under water deficit stress 总被引:1,自引:0,他引:1
Rasheed Rizwan Yasmeen Humaira Hussain Iqbal Iqbal Muhammad Ashraf Muhammad Arslan Parveen Abida 《Physiology and Molecular Biology of Plants》2020,26(3):489-499
Physiology and Molecular Biology of Plants - The present experiment was set-up to appraise protective role of ALA in sunflower cultivars (FH-1581 and FH-1572) under water scarcity stress.... 相似文献
23.
Sahar Mumtaz Muhammad Hamzah Saleem Mansoor Hameed Fatima Batool Abida Parveen Syeda Fasiha Amjad Athar Mahmood Muhammad Arfan Shakeel Ahmed Humaira Yasmin Abdulaziz Abdullah Alsahli Mohammed Nasser Alyemeni 《Saudi Journal of Biological Sciences》2021,28(5):2655-2666
Salinity is extremely hazardous to agriculture worldwide and its expanding constantly. Soil of almost 100 countries facing salinity problem including Pakistan. Cyperus laevigatus also act as salinity indicator species is a naturally adapted halophyte dispersed in subtropical regions of world. Six populations of C. laevigatus were collected from different saline habitats to evaluate adaptations regarding anatomical and physiological characteristics. C. laevigatus is perfectly adapted to harsh environmental conditions like dry barren soils, saline lakes, hyper-saline wetlands and salt marshes. Ecological success of this species is due to plasticity in physiological and anatomical characteristics to adapt variable environmental conditions. C. laevigatus is a halophyte, exhibited increased biomass production in moderately saline habitat. Higher uptake of K+ occurs to compensate the uptake of Na+ ion contents, a striking feature of salt-tolerant and halophytic species. Accumulation of osmoprotectants like proline, free amino acids, soluble sugar and protein contribute significantly to osmotic adjustment. Stem thickness enhanced as salinity level of habitat increased to store water in parenchymatous tissues under physiological drought. Intensive sclerification in root cortex provide mechanical strength to plant as well as prevent the radial leakage of water. Well-developed aerenchyma, increased vascular bundle area, broader vessels, small and dense stomata are critical to cope with environmental hazards. Population of Jahlar lake showing maximum biomass production indicate that this species grows better in moderate salinities. Therefore, this species will prove very useful for revegetation of salt affected rangeland and prairies by direct growth of such halophytic ecotypes. 相似文献
24.
Abida Yasmeen Bushra Mirza Samia Inayatullah Naila Safdar Maryam Jamil Shawkat Ali M. Fayyaz Choudhry 《Plant Molecular Biology Reporter》2009,27(1):20-28
In this study, in planta transformation of tomato (Solanum lycopersicum L.), using fruit injection and floral dip, is reported. Agrobacterium tumefaciens strain EHA 105 containing one of three constructs, i.e., pROKIIAP1GUSint (carrying the Apetala 1 [AP1] gene), pROKIILFYGUSint (carrying the LEAFY [LFY] gene), or p35SGUSint (carrying the β-glucuronidase [GUS] gene), was used for plant transformation. For fruit injection transformation, no significant effects (p > 0.05) of the construct used were observed. The highest frequency of transformation was obtained following 48-h incubation
of tomato fruit with bacterial cells harboring either one of the three constructs; transformation frequencies of 17%, 19%,
and 21% for AP1, LFY, and GUS gene constructs, respectively, were obtained. When fruit maturity was evaluated in fruit injection experiments, mature red
fruit resulted in higher frequency of transformants than immature green fruit with 40%, 35%, and 42% for AP1, LFY, and GUS gene constructs, respectively. For floral dip transformation, a higher number of transformants was obtained when the GUS gene construct was used instead of either the AP1 or LFY gene construct, thus suggesting a possible inhibitory effect of the flowering genes used. When flowers were transformed prior
to rather than following pollination, they yielded a higher transformation frequency, 12% for the LFY construct and 23% for the GUS construct (p < 0.05), although no transformant was obtained with the AP1 gene construct. All putative GUS-positive transformants were analyzed using polymerase chain reaction and confirmed for the
presence of the transgene. Compared to control plants, transgenic plants carrying either the AP1 or LFY transgene flowered earlier and showed several different morphological characters. 相似文献
25.
Mengual R El Abida K Mouaffak N Rieu M Beaudry M 《American journal of physiology. Endocrinology and metabolism》2003,285(6):E1196-E1204
The specificity of the transport mechanisms for pyruvate and lactate and their sensitivity to inhibitors were studied in L6 skeletal muscle cells. Trans- and cis-lactate effects on pyruvate transport kinetic parameters were examined. Pyruvate and lactate were transported by a multisite carrier system, i.e., by two families of sites, one with low affinity and high capacity (type I sites) and the other with high affinity and low capacity (type II). The multisite character of transport kinetics was not modified by either hydroxycinnamic acid (CIN) or p-chloromercuribenzylsulfonic acid (PCMBS), which exert different types of inhibition. The transport efficiency (TE) ratios of maximal velocity to the trans-activation dissociation constant (Kt) showed that lactate and pyruvate were preferentially transported by types I and II sites, respectively. The cis-lactate effect was observed with high Ki values for both sites. The trans-lactate effect on pyruvate transport occurred only on type I sites and exhibited an asymmetric interaction pattern (Kt of inward lactate > Kt of outward lactate). The inability of lactate to trans-stimulate type II sites suggests that intracellular lactate cannot recruit these sites. The high-affinity type II sites act as a specific pyruvate shuttle and constitute an essential relay for the intracellular lactate shuttle. 相似文献
26.
Beaudry M Mouaffak N Darribere T El Abida K Rieu M Mengual R 《The Journal of membrane biology》2000,173(2):89-95
Lactate transport was investigated in newborn rat muscle cells in culture. The aim was to study the lactate transport function
at two stages of cell differentiation in culture: (i) during the proliferative phase characterized by myoblasts and myotubes
(MyB/MyT2) obtained after 2–3 seedings, (ii) when myotubes (MyT1) grow old in culture after 8–9 seedings. In both developmental
stages MyB/MyT2, lactate was carried following a saturable and sigmoidal velocity curve: the Hill and the Scatchard plot analyses
confirmed an allosteric or multisite mechanism of lactate transport with two classes of carriers: one of low and one of high
affinity i.e., 8.6 and 0.95 mm, respectively, which are associated with high and low transport capacities (V
m
) i.e., 9.1 and 0.67 nm/min/mg, respectively. With MyT1, the velocity curve of lactate transport presented a hyperbolic profile, and the Hill plot
analysis gave a Hill number near one suggesting that for cell aging in culture the decrease in cooperativity shows that lactate
transport essentially occurs through the low affinity transport system. Inhibitor effects also contributed to evidence for
at least two systems of transport. Results obtained from primary cells give evidence for the early activity of lactate transport
system at the Myb/MyT2 stage and its evolution during cell aging in culture (MyT1). Sarcolemmal lactate transport in primary
cultures of myocytes is accomplished by multiple carriers, neither of which are MCT1 or MCT2 as confirmed by immunoblots.
Received: 31 March 1999/Revised: 22 September 1999 相似文献
27.
Naima Asghar Abida Aziz Muhammad Farooq Azhar Mohamed El-Sharnouby Uzma Irfan Iqra Rafiq Husnain Farooq Muhammad Ishaq Asif Rehmani Mohamed I. Sakran A. Altalhi Fahad M. Alzuaibr Ayman El Sabagh 《Phyton》2022,91(8):1817-1829
Moringa oleifera is a miracle plant rich in nutrients, antioxidants, and antibiotic properties. Present study was
designed to evaluate various biochemical attributes of leaves and flowers of M. oleifera. Plant parts (leaves, flowers) of M. oleifera, collected from different roadsides of Multan district, Punjab, Pakistan, were used as experimental material. Result indicates that alkaloids, saponin, carbohydrates, fats, and protein had a high value in the
aqueous extract of both leaves and flowers of M. oleifera. Whereas phenol content was high in methanolic leaves
extract and the phenol contents were high in aqueous extract of flowers. The extract yield of M. oleifera leaves and
flowers both showed a higher percentage in aqueous extract (57.5%), followed by methanol extract and lowest in
ethyl acetate extract. Flavonoids contents were higher in ethyl acetate extract of leaves (33.67%) and aqueous
extract of flowers (53.71%). While crude fiber was high in methanolic extract of leaves (12.40%) and in flowers
crude fiber was high in ethyl acetate extract (15.86%). The moisture contents were higher in leaves (8.87%) than
flowers (7.3%) and similarly, ash percentage in flowers (52.60%) than leaves (41.84%). Ethyl acetate extracts of
M. oleifera leaves show antibacterial activity against Pseudomonas aeruginosa while methanolic extract of M. oleifera flowers shows antibacterial activity against Xanthomonas sp. Maximum growth inhibits show in all extracts of
leaves against Aspergillus flavus, F. oxysporum, and P. glabrum except for the concentrated aqueous extract of
leaves. While in flowers maximum growth inhibits all extracts against P. glabrum, A. niger, and A. flavus except
the diluted ethyl acetate extract. Phytochemicals present in different parts of moringa have significant edible and
commercial potential. Moringa extracts exhibited significant antimicrobial activity, therefore have applications in
pharmaceuticals. 相似文献
28.
Clamagirand C El Abida B Der Garabedian PA Hanquez C Dubost L Marie A Rholam M Friguet B Cohen P 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2007,146(4):530-539
Using a monoclonal antibody against the entire C-terminal end of human APP695 (643–695 sequence) and a monoclonal antibody directed against human β[1–40] amyloid peptide (βA), we show the existence of endogenous peptides proteolytically derived from APP in skin exudate of the non transgenic Xenopus laevis frog. The majority of the immunoreactivity is found associated with a 30 kDa molecular species. Biochemical fractionation followed by mass spectrometry identification allowed us to assign this molecular species to C-terminal APP fragments containing all or part of βA. According to the nature of N- and C-terminal amino acids we identified endogenous β-, γ-, ε-secretase-like activities, caspase-like activity and numerous endogenous cleavage sites within the β-amyloid sequence at same sites as those observed in human βA sequence. All these homologies with human indicate that X. laevis skin exudate is a good natural model to study βA metabolism. In this way, interestingly, we identified endogenous cleavages at prohormone convertase-like sites not yet described at the same sites in human. Finally, all identified peptide fragments were stably associated with a 20.2 kDa protein. These new observed features suggest new research pathways concerning human βA metabolism and carriage of hydrophobic peptide fragments issued from APP processing. 相似文献
29.
Due to their importance for human nutrition, the protein content of cereal grains has been a subject of intense study for over a century and cereal grains were not surprisingly one of the earliest subjects for 2D-gel-based proteome analysis. Over the last two decades, countless cereal grain proteomes, mostly derived using 2D-gel based technologies, have been described and hundreds of proteins identified. However, very little is still known about post-translational modifications, subcellular proteomes, and protein-protein interactions in cereal grains. Development of techniques for improved extraction, separation and identification of proteins and peptides is facilitating functional proteomics and analysis of sub-proteomes from small amounts of starting material, such as seed tissues. The combination of proteomics with structural and functional analysis is increasingly applied to target subsets of proteins. These “next-generation” proteomics studies will vastly increase our depth of knowledge about the processes controlling cereal grain development, nutritional and processing characteristics. 相似文献
30.
Majumder A Sultan A Jersie-Christensen RR Ejby M Schmidt BG Lahtinen SJ Jacobsen S Svensson B 《Proteomics》2011,11(17):3470-3481
Lactobacillus acidophilus NCFM is a probiotic bacterium adapted to survive in the gastrointestinal tract and with potential health benefits to the host. Lactitol is a synthetic sugar alcohol used as a sugar replacement in low calorie foods and selectively stimulating growth of L. acidophilus NCFM. In the present study the whole-cell extract proteome of L. acidophilus NCFM grown on glucose until late exponential phase was resolved by 2-DE (pH 3-7). A total of 275 unique proteins assigned to various physiological processes were identified from 650 spots. Differential 2-DE (DIGE) (pH 4-7) of L. acidophilus NCFM grown on glucose and lactitol, revealed 68 spots with modified relative intensity. Thirty-two unique proteins were identified in 41 of these spots changing 1.6-12.7-fold in relative abundance by adaptation of L. acidophilus NCFM to growth on lactitol. These proteins included β-galactosidase small subunit, galactokinase, galactose-1-phosphate uridylyltransferase and UDP-glucose-4-epimerase, which all are potentially involved in lactitol metabolism. This first comprehensive proteome analysis of L. acidophilus NCFM provides insights into protein abundance changes elicited by the prebiotic lactitol. 相似文献