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Vitthal Awad Rohit Shirke Sourav Mukherjee Suresh Khadke Pankaj Pawar Nilambika Meti Abhay Harsulkar 《In vitro cellular & developmental biology. Plant》2011,47(4):525-535
Taverniera cuneifolia (Roth) Arn. or Indian licorice is considered to be a substitute for Glycyrrhiza glabra L. owing to an equivalent content of glycyrrhizic acid (GA). GA recognized as the main active ingredient of T. cuneifolia, GA imparts several medicinal properties to these plants. However, research on this plant is scanty with no published record
on tissue culture studies. Present study demonstrates a method for (1) induction and successful development of somatic embryos
from the root culture, (2) regeneration of plants, and (3) GA production by root cultures of T. cuneifolia. Shoot initiation frequency of cultured roots ranged from 52.57% to 97.71%. Plant regeneration frequency (germination) from
somatic embryos was 88.66% in 1/4-strength Murashige and Skoog (MS) medium supplemented with 2% sucrose, as against 68% in
half 1/2-strength MS containing 2% sucrose. Glycyrrhizic acid of 1.32 mg/gm of dry weight was obtained in full-strength MS
medium supplemented with 3% sucrose, through high-performance thin layer chromatography analysis with standard GA in root
cultures. The present study provides an efficient method for the mass production of plants from a single mother plant as well
as the potential root culture system to study the GA production pathways in T. cuneifolia. 相似文献