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31.
Metals and metalloids were known to have a real problem and serious risk to the environment and the ecosystems as well as human beings. In the present experiment, phyototoxicity of essential metals (Cu and Ni) and non-essential metalloids (As) at 10, 50, 100 and 200 mg L?1 were evaluated on seed germination and early seedlings growth of fenugreek. Higher concentrations of Cu had almost no effect on the parameters tested. By contrast, As was found to reduce germination, root and shoot elongation, seeds vigor index and dry biomass within range dose of 50–200 mg L?1. Accordingly, tolerance index showed that fenugreek seeds have zero tolerance at higher concentration of As. The decreasing order of sensibility of fenugreek seeds to tested metals and metalloid followed the order: As > Ni > Cu. 相似文献
32.
Differential Cytotoxic Activity of Essential Oil of Lippia citriodora from Different Regions in Morocco 下载免费PDF全文
Moulay Ali Oukerrou Mounir Tilaoui Hassan Ait Mouse Najat Bouchmaa Abdelmajid Zyad 《化学与生物多样性》2017,14(7)
The aim of this work was to investigate the cytotoxic effect of the essential oil of dried leaves of Lippia citriodora (H.B. & K.) harvested in different regions of Morocco. This effect was evaluated against the P815 murine mastocytoma cell line using the MTT assay. Interestingly, this work demonstrated for the first time that these essential oils exhibited a strong cytotoxic activity against the P815 cell line, with IC50 values ranging from 7.75 to 13.25 μg/ml. This cytotoxicity began early and increased in a dose‐ and time‐dependent manner. The chemical profile of these essential oils was analyzed by gas chromatography coupled to mass spectrometry. Importantly, the difference in terms of major components’ contents was not significant suggesting probably that the differential cytotoxicity between these essential oils could be attributed to the difference in the content of these essential oils in minor compounds, which could interact with each other or with the main molecules. Finally, this study demonstrated for the first time that essential oils of L. citriodora from different regions in Morocco induced apoptosis against P815 tumor cell line. 相似文献
33.
Sabile Abdelmajid Poras Isabelle Cherif Dora Goodfellow Peter Avner Philip 《Mammalian genome》1997,8(2):81-85
Mouse/human somatic cell hybrids constitute a valuable resource for both genetic and physical mapping. In this report, we
describe the production and characterization of a series of six monochromosomal hybrids generated by fusion of murine microcells
with intact human recipient cells. The presence of each mouse chromosome was characterized by PCR analysis and the integrity
of the mouse chromosome retained in the hybrids confirmed by fluorescence in situ hybridization (FISH) analysis.
Received: 22 August 1996 / Accepted: 19 September 1996 相似文献
34.
Skirycz A Memmi S De Bodt S Maleux K Obata T Fernie AR Devreese B Inzé D 《Journal of proteome research》2011,10(3):1018-1029
35.
Cardol P Boutaffala L Memmi S Devreese B Matagne RF Remacle C 《Biochimica et biophysica acta》2008,1777(4):388-396
In the green alga Chlamydomonas reinhardtii, a mutant deprived of complex I enzyme activity presents a 1T deletion in the mitochondrial nd5 gene. The loss of the ND5 subunit prevents the assembly of the 950 kDa whole complex I. Instead, a low abundant 700 kDa subcomplex, loosely associated to the inner mitochondrial membrane, is assembled. The resolution of the subcomplex by SDS-PAGE gave rise to 19 individual spots, sixteen having been identified by mass spectrometry analysis. Eleven, mainly associated to the hydrophilic part of the complex, are homologs to subunits of the bovine enzyme whereas five (including gamma-type carbonic anhydrase subunits) are specific to green plants or to plants and fungi. None of the subunits typical of the beta membrane domain of complex I enzyme has been identified in the mutant. This allows us to propose that the truncated enzyme misses the membrane distal domain of complex I but retains the proximal domain associated to the matrix arm of the enzyme. A complex I topology model is presented in the light of our results. Finally, a supercomplex most probably corresponding to complex I-complex III association, was identified in mutant mitochondria, indicating that the missing part of the enzyme is not required for the formation of the supercomplex. 相似文献
36.
Haouane H El Bakkali A Moukhli A Tollon C Santoni S Oukabli A El Modafar C Khadari B 《Genetica》2011,139(9):1083-1094
The conservation of cultivated plants in ex-situ collections is essential for the optimal management and use of their genetic
resources. For the olive tree, two world germplasm banks (OWGB) are presently established, in Córdoba (Spain) and Marrakech
(Morocco). This latter was recently founded and includes 561 accessions from 14 Mediterranean countries. Using 12 nuclear
microsatellites (SSRs) and three chloroplast DNA markers, this collection was characterised to examine the structure of the
genetic diversity and propose a set of olive accessions encompassing the whole Mediterranean allelic diversity range. We identified
505 SSR profiles based on a total of 210 alleles. Based on these markers, the genetic diversity was similar to that of cultivars
and wild olives which were previously characterised in another study indicating that OWGB Marrakech is representative of Mediterranean
olive germplasm. Using a model-based Bayesian clustering method and principal components analysis, this OWGB was structured
into three main gene pools corresponding to eastern, central and western parts of the Mediterranean Basin. We proposed 10
cores of 67 accessions capturing all detected alleles and 10 cores of 58 accessions capturing the 186 alleles observed more
than once. In each of the 10 cores, a set of 40 accessions was identical, whereas the remaining accessions were different,
indicating the need to include complementary criteria such as phenotypic adaptive and agronomic traits. Our study generated
a molecular database for the entire OWGB Marrakech that may be used to optimise a strategy for the management of olive genetic
resources and their use for subsequent genetic and genomic olive breeding. 相似文献
37.
James Tolchard Manoj Kumar Pandey Mélanie Berbon Abdelmajid Noubhani Sven J Saupe Yusuke Nishiyama Birgit Habenstein Antoine Loquet 《Journal of biomolecular NMR》2018,70(3):177-185
We present a new solid-state NMR proton-detected three-dimensional experiment dedicated to the observation of protein proton side chain resonances in nano-liter volumes. The experiment takes advantage of very fast magic angle spinning and double quantum 13C–13C transfer to establish efficient (H)CCH correlations detected on side chain protons. Our approach is demonstrated on the HET-s prion domain in its functional amyloid fibrillar form, fully protonated, with a sample amount of less than 500 µg using a MAS frequency of 70 kHz. The majority of aliphatic and aromatic side chain protons (70%) are observable, in addition to Hα resonances, in a single experiment providing a complementary approach to the established proton-detected amide-based multidimensional solid-state NMR experiments for the study and resonance assignment of biosolid samples, in particular for aromatic side chain resonances. 相似文献
38.
A gene for photoactive yellow protein (PYP) was identified from the genome sequence of the extremely halophilic aerobic bacterium Salinibacter ruber (Sr). The sequence is distantly related to the prototypic PYP from Halorhodospira halophila (Hh) (37% identity) and contains most of the amino acid residues identified as necessary for function. However, the Sr pyp gene is not flanked by its two biosynthetic genes as in other species. To determine as to whether the Sr pyp gene encodes a functional protein, we cloned and expressed it in Escherichia coli, along with the genes for chromophore biosynthesis from Rhodobacter capsulatus. The Sr PYP has a 31-residue N-terminal extension as compared to other PYPs that appears to be important for dimerization; however, truncation of these extra residues did not change the spectral and photokinetic properties. Sr PYP has an absorption maximum at 431 nm, which is at shorter wavelengths than the prototypical Hh PYP (at 446 nm). It is also photoactive, being reversibly bleached by either blue or white light. The kinetics of dark recovery is slower than any of the PYPs reported to date (4.27 x 10(-4) s(-1) at pH 7.5). Sr PYP appears to have a normal photocycle with the I1 and I2 intermediates. The presence of the I2' intermediate is also inferred on the basis of the effects of temperature and alchohol on recovery. Sr PYP has an intermediate spectral form in equilibrium with the 431 nm form, similar to R. capsulatus PYP and the Y42F mutant of Hh PYP. Increasing ionic strength stabilizes the 431 nm form at the expense of the intermediate spectral form, and the kinetics of recovery is accelerated 6.4-fold between 0 and 3.5 M salt. This is observed with ions from both the chaotropic and the kosmotropic series. Ionic strength also stabilizes PYP against thermal denaturation, as the melting temperature is increased from 74 degrees C in buffer alone to 92 degrees C in 2 M KCl. Sr accumulates KCl in the cytoplasm, like Halobacterium, to balance osmotic pressure and has very acidic proteins. We thus believe that Sr PYP is an example of a halophilic protein that requires KCl to electrostatically screen the excess negative charge and stabilize the tertiary structure. 相似文献
39.
Burcu Koçak Memmi 《Journal of vector ecology》2010,35(1):144-148
In this study, the knockdown and mortality effects of imidacloprid and methomyl were investigated. The residual surface applications were carried out to determine the knockdown effects (KDt50 and KDt95) and mortality (LD50 and LD95) induced by each insecticide. For mortality comparisons, the susceptible house fly (Musca domestica L., Diptera: Muscidae) of a WHO population and three natural field‐collected M. domestica populations from Turkey were used. In conclusion, it was found that the resistance to imidacloprid and methomyl was significantly higher in the field populations when compared to the susceptible population from WHO. The results showed that applicators and pest management decision‐makers should control and conduct an integrated pest management strategy by including biological agents to prevent the development of high levels of resistance in the field populations. 相似文献
40.
Nair D Memmi G Hernandez D Bard J Beaume M Gill S Francois P Cheung AL 《Journal of bacteriology》2011,193(9):2332-2335
Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies. 相似文献