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111.
Corneal angiogenesis and lymphangiogenesis are induced by vascular endothelial growth factors (VEGFs) signaling through its receptors VEGFR-1, -2, and -3. Endostatin is a peptide antagonist of these receptors that causes inhibition of bFGF-induced corneal angiogenesis and lymphangiogenesis. Here we show that binding of VEGF-C and endostatin to recombinant VEGFR-3 is competitive. Alignments of the primary amino acid sequences of VEGF-C and the C-terminal endostatin peptide (mEP: LEQKAASCHNSYIVLCIENSFMTSFSK) identified two conserved cysteine residues separated by seven amino acids. Peptides of VEGF-C and mEP containing these conserved residues bound toVEGFR-3. However, substitution of alanine for either of the cysteines in the mEP peptide perturbed the secondary structure, and this mutated peptide was unable to bind to VEGFR-3. Analysis by surface plasmon resonance demonstrated that the binding of the mEP peptide for recombinant VEGFR-3 had a Ka of 1.41x107M-1s-1, Kd of 0.6718 s-1, and a KD of 4.78x10-8M. Characterization of the mechanism of endostatin binding to VEGFR-3 may lead to the development of novel therapies for lymphangiogenesis-related disorders, such as transplant rejection, lymphedema, and cancer metastasis. 相似文献
112.
Najmanovich RJ Allali-Hassani A Morris RJ Dombrovsky L Pan PW Vedadi M Plotnikov AN Edwards A Arrowsmith C Thornton JM 《Bioinformatics (Oxford, England)》2007,23(2):e104-e109
MOTIVATION: In the present work we combine computational analysis and experimental data to explore the extent to which binding site similarities between members of the human cytosolic sulfotransferase family correlate with small-molecule binding profiles. Conversely, from a small-molecule point of view, we explore the extent to which structural similarities between small molecules correlate to protein binding profiles. RESULTS: The comparison of binding site structural similarities and small-molecule binding profiles shows that proteins with similar small-molecule binding profiles tend to have a higher degree of binding site similarity but the latter is not sufficient to predict small-molecule binding patterns, highlighting the difficulty of predicting small-molecule binding patterns from sequence or structure. Likewise, from a small-molecule perspective, small molecules with similar protein binding profiles tend to be topologically similar but topological similarity is not sufficient to predict their protein binding patterns. These observations have important consequences for function prediction and drug design. 相似文献
113.
Mohammed Hassi Abdellatif Haggoud Mohammed El Mzibri Saad Ibnsouda Abdellah Houari Mohammed Iraqui 《Annals of microbiology》2007,57(4):651-656
The resistance of mycobacteria to the clinical applied antibiotics poses a serious problem to deal with the infections they cause. So, the search for new antibiotics active against these bacteria becomes urgent. We report here the isolation from a Moroccan biotope of a bacterial strain secreting an active substance of protein nature that inhibits the growth of several mycobacterial species (Mycobacterium smegmatis; M. aurum A+;M. vaccae; M. bovis BCG andM. kansasii). PCR amplification and DNA sequecing of the 16S ribosomal RNA gene allowed the identification of this strain asStaphylococcus haemolyticus. Moreover, the substance produced by this strain was able to lyse the wall ofM. smegmatis and to extract its genomic DNA indicating that it acts probably, like others anti-mycobacterial antibiotics, on this envelope. The identification and characterisation of the active substance would open the way for further technological and therapeutic investigations. 相似文献
114.
La Carbona S Sauvageot N Giard JC Benachour A Posteraro B Auffray Y Sanguinetti M Hartke A 《Molecular microbiology》2007,66(5):1148-1163
The opportunistic pathogen Enterococcus faecalis is well equipped with peroxidatic activities. It harbours three loci encoding a NADH peroxidase, an alkyl hydroperoxide reductase and a protein (EF2932) belonging to the AhpC/TSA family. We present results demonstrating that ef2932 does encode a thiol peroxidase (Tpx) and show that it is part of the regulon of the hydrogen peroxide regulator HypR. Characterization of unmarked deletion mutants showed that all three peroxidases are important for the defence against externally provided H(2)O(2). Exposure to internal generated H(2)O(2) by aerobic growth on glycerol, lactose, galactose or ribose showed that Npr was absolutely required for aerobic growth on glycerol and optimal growth on the other substrates. Growth on glycerol was also dependent on Ahp. Addition of catalase restored growth of the mutants, and therefore, extracellular H(2)O(2) concentrations have been determined. This showed that the time point of growth arrest of the Deltanpr mutant correlated with the highest H(2)O(2) concentration measured. Analysis of the survival of the different strains inside peritoneal macrophages revealed that Tpx was the most important antioxidant activity for protecting the cells against the hostile phagocyte environment. Finally, the Deltatpx and the triple mutant showed attenuated virulence in a mouse peritonitis model. 相似文献
115.
David McGowan Origène Nyanguile Maxwell D. Cummings Sandrine Vendeville Koen Vandyck Walter Van den Broeck Carlo W. Boutton Hendrik De Bondt Ludo Quirynen Katie Amssoms Jean-François Bonfanti Stefaan Last Klara Rombauts Abdellah Tahri Lili Hu Frédéric Delouvroy Katrien Vermeiren Geneviève Vandercruyssen Liesbet Van der Helm Erna Cleiren Pierre Raboisson 《Bioorganic & medicinal chemistry letters》2009,19(9):2492-2496
Optimization through parallel synthesis of a novel series of hepatitis C virus (HCV) NS5B polymerase inhibitors led to the identification of (R)-11-(4-benzyloxy-2-fluorophenyl)-6-hydroxy-3,3-dimethyl-10-(6-methylpyridine-2-carbonyl)-2,3,4,5,10,11-hexahydro-dibenzo[b,e][1,4]diazepin-1-one 11zc and (R)-11-(4-benzyloxy-2-fluorophenyl)-6-hydroxy-3,3-dimethyl-10-(2,5-dimethyloxazol-4-carbonyl)-2,3,4,5,10,11-hexahydro-dibenzo[b,e][1,4]diazepin-1-one 11zk as potent (replicon EC50 = 400 nM and 270 nM, respectively) and selective (CC50 > 20 μM) inhibitors of HCV replication. These data warrant further lead-optimization efforts. 相似文献
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Abdellah Amimi Aziza Mouradi Laila Bennasser Thiery Givernaud 《Phycological Research》2007,55(2):143-149
The biology and biochemistry of Gigartina pistillata (Gmelin) Stackhouse collected monthly at Nation Beach (Morocco), was studied during one year. The biological study showed one period of active growth from April to July. The thallus composition was quite stable during the major part of the year. The dry matter was maximum in May and August and minimum in January. The maximum carrageenan content occurred in June and September (about 37%) and the minimum carrageenan content occurred in February (19.0%). The total nitrogen content varied significantly, with a maximum in January (1.98%) and a minimum in August (0.7%). The ash content was significant (23–32%) with a maximum in August and a minimum in May. The carrageenan extracted from natural populations of Gigartina pistillata was a mixture of lambda‐type and kappa‐type carrageenans. The 3,6‐anhydrogalactose varied between 4.5 mol% in June to 25 mol% in February. For industrial applications the extract could be considered as a lambda‐type. The best period for harvest of G. pistillata in Morocco is between July and August when biomass and viscosity are at their maximum. A relationship between the physical characteristics of G. pistillata carrageenans and its seasonal cycle was deduced. 相似文献
119.
Human HDAC7 harbors a class IIa histone deacetylase-specific zinc binding motif and cryptic deacetylase activity 总被引:3,自引:0,他引:3
120.
Tomasz Korzeniak Corine Mathonière Abdellah Kaiba Philippe Guionneau Marcin Koziel Barbara Sieklucka 《Inorganica chimica acta》2008,361(12-13):3500-3504
Ionic triads formed by [NiII(bipy)3]2+ (bipy = 2,2′-bipyridyl) and diamagnetic [MIV(CN)8]4? (M = Mo and W) were prepared and structurally characterized. The two compounds are isostructural and their structure consists of a three-dimensional hydrogen-bonded framework where cation–anion interactions occur through short contacts M–CN?H–C(bipy). Before irradiation, the Mo analogue behaves as paramagnet with small intermolecular interactions between the [NiII(bipy)3]2+ cations. Upon irradiation with visible light, it exhibits a reversible photomagnetic effect, which is interpreted in terms of the formation of paramagnetic [MoV(CN)8]3? and [NiII(bipy)2(bipy?)]+ due to the outer-sphere electron transfer. 相似文献