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101.
Badji B Mostefaoui A Sabaou N Lebrihi A Mathieu F Seguin E Tillequin F 《Journal of industrial microbiology & biotechnology》2007,34(6):403-412
An actinomycete strain NM94 was isolated from a Saharan soil sample by a dilution agar plating method using chitin-vitamins
B medium supplemented with penicillin. The strain presented the morphological and chemical characteristics of the genus Nonomuraea. On the basis of 16S rDNA analysis and physiological tests, this isolate was found to be quite different from the known species
of Nonomuraea and might be new. The strain NM94 secreted several antibiotics on yeast extract malt extract glucose medium that were active
against some Gram-positive bacteria, yeast, and fungi. The antibiotics were extracted with dichloromethane and detected by
bioautography on silica gel plates using Mucor
ramannianus and Bacillus
subtilis as the test organisms. Among these antibiotics, a complex called 94A showed interesting antifungal activity. It was selected
and purified by reverse-phase HPLC. This complex was composed of five compounds. Spectroscopic studies by infrared, mass,
and 1H NMR of the compounds were carried out. Initial results showed that these molecules differed from the known antibiotics produced
by other Nonomuraea species. 相似文献
102.
Paul Daly Christopher McClellan Marta Maluk Helena Oakey Catherine Lapierre Robbie Waugh Jennifer Stephens David Marshall Abdellah Barakate Yukiko Tsuji Geert Goeminne Ruben Vanholme Wout Boerjan John Ralph Claire Halpin 《Plant biotechnology journal》2019,17(3):594-607
Caffeic acid O‐methyltransferase (COMT), the lignin biosynthesis gene modified in many brown‐midrib high‐digestibility mutants of maize and sorghum, was targeted for downregulation in the small grain temperate cereal, barley (Hordeum vulgare), to improve straw properties. Phylogenetic and expression analyses identified the barley COMT orthologue(s) expressed in stems, defining a larger gene family than in brachypodium or rice with three COMT genes expressed in lignifying tissues. RNAi significantly reduced stem COMT protein and enzyme activity, and modestly reduced stem lignin content while dramatically changing lignin structure. Lignin syringyl‐to‐guaiacyl ratio was reduced by ~50%, the 5‐hydroxyguaiacyl (5‐OH‐G) unit incorporated into lignin at 10‐–15‐fold higher levels than normal, and the amount of p‐coumaric acid ester‐linked to cell walls was reduced by ~50%. No brown‐midrib phenotype was observed in any RNAi line despite significant COMT suppression and altered lignin. The novel COMT gene family structure in barley highlights the dynamic nature of grass genomes. Redundancy in barley COMTs may explain the absence of brown‐midrib mutants in barley and wheat. The barley COMT RNAi lines nevertheless have the potential to be exploited for bioenergy applications and as animal feed. 相似文献
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Hassan Ghazal Yagoub Adam Abdellah Idrissi Azami Sofia Sehli Hannah N. Nyarko Bouchra Chaouni Grace Olasehinde Itunuoluwa Isewon Marion Adebiyi Olayinka Ajani Enock Matovu Olawole Obembe Yvonne Ajamma Gaston Kuzamunu Samson Pandam Salifu Jonathan Kayondo Alia Benkahla Ezekiel Adebiyi 《The Plant journal : for cell and molecular biology》2021,107(1):21-36
Plants are the world’s most consumed goods. They are of high economic value and bring many health benefits. In most countries in Africa, the supply and quality of food will rise to meet the growing population’s increasing demand. Genomics and other biotechnology tools offer the opportunity to improve subsistence crops and medicinal herbs in the continent. Significant advances have been made in plant genomics, which have enhanced our knowledge of the molecular processes underlying both plant quality and yield. The sequencing of complex genomes of African plant species, facilitated by the continuously evolving next-generation sequencing technologies and advanced bioinformatics approaches, has provided new opportunities for crop improvement. This review summarizes the achievements of genome sequencing projects of endemic African plants in the last two decades. We also present perspectives and challenges for future plant genomic studies that will accelerate important plant breeding programs for African communities. These challenges include a lack of basic facilities, a lack of sequencing and bioinformatics facilities, and a lack of skills to design genomics studies. However, it is imperative to state that African countries have become key players in the plant genome revolution and genome derived-biotechnology. Therefore, African governments should invest in public plant genomics research and applications, establish bioinformatics platforms and training programs, and stimulate university and industry partnerships to fully deploy plant genomics, particularly in the fields of agriculture and medicine. 相似文献
106.
Vedadi M Barsyte-Lovejoy D Liu F Rival-Gervier S Allali-Hassani A Labrie V Wigle TJ Dimaggio PA Wasney GA Siarheyeva A Dong A Tempel W Wang SC Chen X Chau I Mangano TJ Huang XP Simpson CD Pattenden SG Norris JL Kireev DB Tripathy A Edwards A Roth BL Janzen WP Garcia BA Petronis A Ellis J Brown PJ Frye SV Arrowsmith CH Jin J 《Nature chemical biology》2011,7(8):566-574
107.
Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation 总被引:1,自引:0,他引:1
Bian C Xu C Ruan J Lee KK Burke TL Tempel W Barsyte D Li J Wu M Zhou BO Fleharty BE Paulson A Allali-Hassani A Zhou JQ Mer G Grant PA Workman JL Zang J Min J 《The EMBO journal》2011,30(14):2829-2842
The SAGA (Spt-Ada-Gcn5 acetyltransferase) complex is an important chromatin modifying complex that can both acetylate and deubiquitinate histones. Sgf29 is a novel component of the SAGA complex. Here, we report the crystal structures of the tandem Tudor domains of Saccharomyces cerevisiae and human Sgf29 and their complexes with H3K4me2 and H3K4me3 peptides, respectively, and show that Sgf29 selectively binds H3K4me2/3 marks. Our crystal structures reveal that Sgf29 harbours unique tandem Tudor domains in its C-terminus. The tandem Tudor domains in Sgf29 tightly pack against each other face-to-face with each Tudor domain harbouring a negatively charged pocket accommodating the first residue alanine and methylated K4 residue of histone H3, respectively. The H3A1 and K4me3 binding pockets and the limited binding cleft length between these two binding pockets are the structural determinants in conferring the ability of Sgf29 to selectively recognize H3K4me2/3. Our in vitro and in vivo functional assays show that Sgf29 recognizes methylated H3K4 to recruit the SAGA complex to its targets sites and mediates histone H3 acetylation, underscoring the importance of Sgf29 in gene regulation. 相似文献
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Rigottier-Gois L Alberti A Houel A Taly JF Palcy P Manson J Pinto D Matos RC Carrilero L Montero N Tariq M Karsens H Repp C Kropec A Budin-Verneuil A Benachour A Sauvageot N Bizzini A Gilmore MS Bessières P Kok J Huebner J Lopes F Gonzalez-Zorn B Hartke A Serror P 《PloS one》2011,6(12):e29023
Spread of antibiotic resistance among bacteria responsible for nosocomial and community-acquired infections urges for novel therapeutic or prophylactic targets and for innovative pathogen-specific antibacterial compounds. Major challenges are posed by opportunistic pathogens belonging to the low GC% gram-positive bacteria. Among those, Enterococcus faecalis is a leading cause of hospital-acquired infections associated with life-threatening issues and increased hospital costs. To better understand the molecular properties of enterococci that may be required for virulence, and that may explain the emergence of these bacteria in nosocomial infections, we performed the first large-scale functional analysis of E. faecalis V583, the first vancomycin-resistant isolate from a human bloodstream infection. E. faecalis V583 is within the high-risk clonal complex 2 group, which comprises mostly isolates derived from hospital infections worldwide. We conducted broad-range screenings of candidate genes likely involved in host adaptation (e.g., colonization and/or virulence). For this purpose, a library was constructed of targeted insertion mutations in 177 genes encoding putative surface or stress-response factors. Individual mutants were subsequently tested for their i) resistance to oxidative stress, ii) antibiotic resistance, iii) resistance to opsonophagocytosis, iv) adherence to the human colon carcinoma Caco-2 epithelial cells and v) virulence in a surrogate insect model. Our results identified a number of factors that are involved in the interaction between enterococci and their host environments. Their predicted functions highlight the importance of cell envelope glycopolymers in E. faecalis host adaptation. This study provides a valuable genetic database for understanding the steps leading E. faecalis to opportunistic virulence. 相似文献
110.
A Siarheyeva G Senisterra A Allali-Hassani A Dong E Dobrovetsky GA Wasney I Chau R Marcellus T Hajian F Liu I Korboukh D Smil Y Bolshan J Min H Wu H Zeng P Loppnau G Poda C Griffin A Aman PJ Brown J Jin R Al-Awar CH Arrowsmith M Schapira M Vedadi 《Structure (London, England : 1993)》2012,20(8):1425-1435
PRMT3, a protein arginine methyltransferase, has been shown to influence ribosomal biosynthesis by catalyzing the dimethylation of the 40S ribosomal protein S2. Although PRMT3 has been reported to be a cytosolic protein, it has been shown to methylate histone H4 peptide (H4 1-24) in?vitro. Here, we?report the identification of a PRMT3 inhibitor (1-(benzo[d][1,2,3]thiadiazol-6-yl)-3-(2-cyclohexenylethyl)urea; compound 1) with IC(50) value of 2.5?μM by screening a library of 16,000 compounds using H4 (1-24) peptide as a substrate. The crystal structure of PRMT3 in complex with compound 1 as well as kinetic analysis reveals an allosteric mechanism of inhibition. Mutating PRMT3 residues within the allosteric site or using compound 1 analogs that disrupt interactions with allosteric site residues both abrogated binding and inhibitory activity. These data demonstrate an allosteric mechanism for inhibition of protein arginine methyltransferases, an emerging class of therapeutic targets. 相似文献