排序方式: 共有112条查询结果,搜索用时 15 毫秒
91.
92.
Dif Guendouz Belaouni Hadj Ahmed Yekkour Amine Goudjal Yacine Djemouai Nadjette Peňázová Eliška Čechová Jana Berraf-Tebbal Akila Eichmeier Ales Zitouni Abdelghani 《World journal of microbiology & biotechnology》2022,38(1):1-10
World Journal of Microbiology and Biotechnology - Beauvericin and bassiatin are two valuable compounds with various bioactivities biosynthesized by the supposedly same nonribosomal peptide... 相似文献
93.
Abdelghani Bounafaa Hicham Berrougui Noreddine Ghalim Boubker Nasser Abdallah Bagri Abderrahmane Moujahid Souad Ikhlef Pamela Camponova Najoua Yamoul Olivier Kamtchueng Simo Abdelkhalid Essamadi Abdelouahed Khalil 《PloS one》2015,10(8)
The purpose of the present study was to investigate the distribution of PON1 Q192R and L55M polymorphisms and activities in a North African population and to determine their association with cardiovascular complications. The prevalence of the QQ, QR, RR, LL, LM, and MM genotypes in the study population was 55.4%, 34.09%, 9.83%, 41.97%, 48.20%, and 9.83% respectively. The Q, R, L, and M alleles had a gene frequency of 0.755, 0.245, 0.67, and 0.33, respectively. The PON1 192 RR genotype was significantly more prevalent among ACS patients than among healthy subjects. There was a 4.33-fold increase in the risk of ACS in subjects presenting the PON1 192 RR genotype compared to those with the QQ genotype (OR=4.33; 95% CI=1.27–17.7). There was a significantly different distribution of PON1 L55M in the ACS patient groups (UA, STEMI, NSTEMI). Moreover, individuals presenting the PON1 55MM genotype present a higher risk for ACS than those with LL genotype (OR=3.69; 95% CI=1.61–11.80). Paraoxonase activities were significantly lower in coronary patients than in healthy subjects. The decrease in PON1 activity was inversely correlated with the number of concomitant risk factors for CVD (r=0.57, p<0.0001). The results of the present study suggested that the PON1 R and M alleles may play a role in the pathogenesis of cardiac ischemia in our North African population and that a decrease in PON1 activity may be a valuable marker for monitoring the development of the atherosclerosis process and the associated cardiovascular complications. 相似文献
94.
Jana Prochazkova Shinya Sakaguchi Michel Owusu Abdelghani Mazouzi Marc Wiedner Georgia Velimezi Martin Moder Gleb Turchinovich Anastasiya Hladik Elisabeth Gurnhofer Adrian Hayday Axel Behrens Sylvia Knapp Lukas Kenner Wilfried Ellmeier Joanna I. Loizou 《PLoS genetics》2015,11(11)
Proper development of the immune system is an intricate process dependent on many factors, including an intact DNA damage response. The DNA double-strand break signaling kinase ATM and its cofactor NBS1 are required during T cell development and for the maintenance of genomic stability. The role of a second ATM cofactor, ATMIN (also known as ASCIZ) in T cells is much less clear, and whether ATMIN and NBS1 function in synergy in T cells is unknown. Here, we investigate the roles of ATMIN and NBS1, either alone or in combination, using murine models. We show loss of NBS1 led to a developmental block at the double-positive stage of T cell development, as well as reduced TCRα recombination, that was unexpectedly neither exacerbated nor alleviated by concomitant loss of ATMIN. In contrast, loss of both ATMIN and NBS1 enhanced DNA damage that drove spontaneous peripheral T cell hyperactivation, proliferation as well as excessive production of proinflammatory cytokines and chemokines, leading to a highly inflammatory environment. Intriguingly, the disease causing T cells were largely proficient for both ATMIN and NBS1. In vivo this resulted in severe intestinal inflammation, colitis and premature death. Our findings reveal a novel model for an intestinal bowel disease phenotype that occurs upon combined loss of the DNA repair cofactors ATMIN and NBS1. 相似文献
95.
Redrejo-Rodríguez M Saint-Pierre C Couve S Mazouzi A Ishchenko AA Gasparutto D Saparbaev M 《PloS one》2011,6(7):e21039
Background
Oxidative damage to DNA, if not repaired, can be both miscoding and blocking. These genetic alterations can lead to mutations and/or cell death, which in turn cause cancer and aging. Oxidized DNA bases are substrates for two overlapping repair pathways: base excision (BER) and nucleotide incision repair (NIR). Hydantoin derivatives such as 5-hydroxyhydantoin (5OH-Hyd) and 5-methyl-5-hydroxyhydantoin (5OH-5Me-Hyd), major products of cytosine and thymine oxidative degradation pathways, respectively, have been detected in cancer cells and ancient DNA. Hydantoins are blocking lesions for DNA polymerases and excised by bacterial and yeast DNA glycosylases in the BER pathway. However little is known about repair of pyrimidine-derived hydantoins in human cells.Methodology/Principal Findings
Here, using both denaturing PAGE and MALDI-TOF MS analyses we report that the bacterial, yeast and human AP endonucleases can incise duplex DNA 5′ next to 5OH-Hyd and 5OH-5Me-Hyd thus initiating the NIR pathway. We have fully reconstituted the NIR pathway for these lesions in vitro using purified human proteins. Depletion of Nfo in E. coli and APE1 in HeLa cells abolishes the NIR activity in cell-free extracts. Importantly, a number of redundant DNA glycosylase activities can excise hydantoin residues, including human NTH1, NEIL1 and NEIL2 and the former protein being a major DNA glycosylase activity in HeLa cells extracts.Conclusions/Significance
This study demonstrates that both BER and NIR pathways can compete and/or back-up each other to remove hydantoin DNA lesions in vivo. 相似文献96.
Yacine Goudjal Miyada Zamoum Nasserdine Sabaou Florence Mathieu Abdelghani Zitouni 《Biocontrol Science and Technology》2016,26(12):1691-1705
Sixteen endophytic actinobacteria isolated from roots of native plants were evaluated for their antagonistic potential against soil-borne phytopathogenic fungi. Among them, three strong antagonistic isolates were selected and characterised for in vitro plant-growth-promoting and biocontrol traits, including production of hydrogen cyanide, indole-3-acetic acid and siderophores, chitinase and β-1,3-glucanase activities, and inorganic phosphate solubilisation. In all trials, the strain Streptomyces sp. SNL2 revealed promising features. The selected actinobacteria were investigated for the biocontrol of Fusarium oxysporum f. sp. radicis lycopersici and for growth promotion of tomato (Solanum lycopersicum L. cv. Aïcha) seedlings in autoclaved and non-autoclaved soils. All seed-bacterisation treatments significantly reduced the root rot incidence compared to a positive control (with infested soil), and the isolate SNL2 exhibiting the highest protective activity. It reduced the disease incidence from 88.5% to 13.2%, whereas chemical seed treatment with Thiram® provided 14.6% disease incidence. Furthermore, isolate SNL2 resulted in significant increases in the dry weight, shoot and root length of seedlings. 16S rDNA sequence analysis showed that isolate SNL2 was related to Streptomyces asterosporus NRRL B-24328T (99.52% of similarity). Its interesting biocontrol potential and growth enhancement of tomato seedlings open up attractive uses of the strain SNL2 in crop improvement. 相似文献
97.
Xavier Fradera Joey L. Methot Abdelghani Achab Matthew Christopher Michael D. Altman Hua Zhou Meredeth A. McGowan Sam D. Kattar Kevin Wilson Yudith Garcia Martin A. Augustin Charles A. Lesburg Sanjiv Shah Peter Goldenblatt Jason D. Katz 《Bioorganic & medicinal chemistry letters》2019,29(18):2575-2580
PI3Kδ mediates key immune cell signaling pathways and is a target of interest for multiple indications in immunology and oncology. Here we report a structure-based scaffold-hopping strategy for the design of chemically diverse PI3Kδ inhibitors. Using this strategy, we identified several scaffolds that can be combined to generate new PI3Kδ inhibitors with high potency and isoform selectivity. In particular, an oxindole-based scaffold was found to impart exquisite selectivity when combined with several hinge binding motifs. 相似文献
98.
Ines Zrafi-Nouira Sonda Guermazi Rakia Chouari Nimer M. D. Safi Eric Pelletier Amina Bakhrouf Dalila Saidane-Mosbahi Abdelghani Sghir 《Biodegradation》2009,20(4):467-486
The indigenous microbiota of polluted coastal seawater in Tunisia was enriched by increasing the concentration of zarzatine
crude oil. The resulting adapted microbiota was incubated with zarzatine crude oil as the only carbon and energy source. Crude
oil biodegradation capacity and bacterial population dynamics of the microbiota were evaluated every week for 28 days (day
7, day 14, day 21, and day 28). Results show that the percentage of petroleum degradation was 23.9, 32.1, 65.3, and 77.8%,
respectively. At day 28, non-aromatic and aromatic hydrocarbon degradation rates reached 92.6 and 68.7%, respectively. Bacterial
composition of the adapted microflora was analysed by 16S rRNA gene cloning and sequencing, using total genomic DNA extracted
from the adapted microflora at days 0, 7, 14, 21, and 28. Five clone libraries were constructed and a total of 430 sequences
were generated and grouped into OTUs using the ARB software package. Phylogenetic analysis of the adapted microbiota shows
the presence of four phylogenetic groups: Proteobacteria,
Firmicutes, Actinobacteria and Bacteroidetes. Diversity indices show a clear decrease in bacterial diversity of the adapted microflora according to the incubation time.
The Proteobacteria are the most predominant (>80%) at day 7, day 14 and day 21 but not at day 28 for which the microbiota was reduced to only
one OTU affiliated with the genus Kocuria of the Actinobacteria. This study shows that the degradation of zarzatine crude oil components depends on the activity of a specialized and dynamic
seawater consortium composed of different phylogenetic taxa depending on the substrate complexity. 相似文献
99.
Boudjella H Bouti K Zitouni A Mathieu F Lebrihi A Sabaou N 《Microbiological research》2006,161(4):288-298
A new actinomycete strain designated Sg 10, producing antimicrobial substances was isolated from an Algerian soil. Morphological and chemical studies indicated that strain Sg 10 belonged to the genus Streptosporangium. The comparison of its physiological characteristics with those of known species of Streptosporangium showed significant differences with the nearest species Streptosporangium carneum. Analysis of the 16S rDNA sequence of strain Sg 10 showed a similarity level ranging between 96.3% and 97.8% within Streptosporangium species, with S. carneum the most closely related. However, the phylogenetic analysis indicated that strain Sg 10 represent a distinct phyletic line suggesting a new genomic species. The antimicrobial activity of strain Sg 10 showed an antibacterial activity against Gram-positive bacteria as well as an antifungal one. Four active products were isolated from the culture broth using various separation procedures. On the basis of UV-VIS spectrometry, infrared spectroscopy and chemical revelations, the antibiotics were classified in the group of glycosylated aromatics. 相似文献
100.
Siala M Jaulhac B Gdoura R Sibilia J Fourati H Younes M Baklouti S Bargaoui N Sellami S Znazen A Barthel C Collin E Hammami A Sghir A 《Arthritis research & therapy》2008,10(2):R40-14