全文获取类型
收费全文 | 1417篇 |
免费 | 80篇 |
国内免费 | 6篇 |
专业分类
1503篇 |
出版年
2024年 | 2篇 |
2023年 | 21篇 |
2022年 | 48篇 |
2021年 | 56篇 |
2020年 | 56篇 |
2019年 | 74篇 |
2018年 | 63篇 |
2017年 | 50篇 |
2016年 | 64篇 |
2015年 | 82篇 |
2014年 | 88篇 |
2013年 | 124篇 |
2012年 | 91篇 |
2011年 | 110篇 |
2010年 | 63篇 |
2009年 | 58篇 |
2008年 | 47篇 |
2007年 | 46篇 |
2006年 | 40篇 |
2005年 | 36篇 |
2004年 | 37篇 |
2003年 | 24篇 |
2002年 | 22篇 |
2001年 | 2篇 |
2000年 | 10篇 |
1999年 | 8篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1995年 | 9篇 |
1994年 | 7篇 |
1993年 | 4篇 |
1992年 | 9篇 |
1991年 | 11篇 |
1990年 | 15篇 |
1989年 | 13篇 |
1988年 | 22篇 |
1987年 | 15篇 |
1986年 | 5篇 |
1985年 | 9篇 |
1984年 | 14篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1975年 | 4篇 |
1957年 | 1篇 |
1956年 | 2篇 |
排序方式: 共有1503条查询结果,搜索用时 15 毫秒
221.
Abbas?Yousefpour Hamid?ModarressEmail author Fatemeh?Goharpey Sepideh?Amjad-Iranagh 《Journal of molecular modeling》2017,23(5):158
The anti-hypertensive drugs amlodipine, atenolol and lisinopril, in ordinary and PEGylated forms, with different combined-ratios, were studied by molecular dynamics simulations using GROMACS software. Twenty simulation systems were designed to evaluate the interactions of drug mixtures with a dimyristoylphosphatidylcholine (DMPC) lipid bilayer membrane, in the presence of water molecules. In the course of simulations, various properties of the systems were investigated, including drug location, diffusion and mass distribution in the membrane; drug orientation; the lipid chain disorder as a result of drug penetration into the DMPC membrane; the number of hydrogen bonds; and drug surface area. According to the results obtained, combined drugs penetrate deeper into the DMPC lipid bilayer membrane, and the lipid chains remain ordered. Also, the combined PEGylated drugs, at a combination ratio of 1:1:1, enhance drug penetration into the DMPC membrane, reduce drug agglomeration, orient the drug in a proper angle for easy penetration into the membrane, and decrease undesirable lipotoxicity due to distorted membrane self-assembly and thickness. 相似文献
222.
Sadegh Feizollahzadeh Reza Ghiasvand Abbas Rezaei Hossein Khanahmad Akram sadeghi Mitra Hariri 《Probiotics and antimicrobial proteins》2017,9(1):41-47
Probiotic therapies are going to be an effective alternative therapeutic strategy in the treatment and management of diabetes. The mechanism behind the essential effects of probiotic therapies in diabetic patients was not fully understood. The objective of this study was to evaluate the effects of probiotic soy milk containing Lactobacillus planetarum A7 on inflammation, lipid profile, fasting blood glucose, and serum adiponectin among patients with type 2 diabetes mellitus. Forty patients with type 2 diabetes, at the age of 35–68 years old, were assigned to two groups in this randomized, double-blind, controlled clinical trial. The patients in the intervention group consumed 200 ml/day of probiotic soy milk containing L. planetarum A7 and those in control group consumed 200 ml/day of pure soy milk for 8 weeks. Serum TNF-α, C reactive protein, adiponectin, lipid profile, and fasting blood glucose were determined before and after intervention. In intervention group, serum adiponectin in pre- and post-treatment did not show any significant changes (2.52 ± 0.74 vs 2.84 ± 0.61, P = 0.658), as well as changes in serum TNF-α and C reactive protein (172.44 ± 5.7 vs 172.83 ± 7.6, P = 0.278, 4.2 ± 1.4 vs 4.5 ± 1.9, P = 0.765, respectively). Low-density cholesterol and high-density cholesterol changed significantly (P = 0.023, P = 0.017, respectively), but fasting blood glucose did not show any significant changes. The results of this study showed that consumption of probiotic soy milk and soy milk has no effect on serum adiponectin and inflammation, but it can change lipid profile among type 2 diabetic patients. 相似文献
223.
Fida Hussain Munawar Iqbal Syed Zahir Shah M. Afzal Qamar Tanveer H. Bokhari Mazhar Abbas Muhammad Younus 《农业工程》2017,37(1):48-52
Gamma radiation, various absorbed doses (0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5 krad) effects were evaluated on sunflower (Helianthus annus. L.) germination and growth characteristics. Sunflower healthy seeds were exposed to gamma radiation source Co60 at nuclear institute for food and agriculture and exposed seeds were grown under controlled laboratory conditions. In comparison to control, gamma radiation absorbed doses affected the measured response positively i.e., radical length, plumule length, number of roots, seedling fresh weight, seedling dry weight, germination percentage, time of germination and diameter of hypocotyl of sunflower enhanced up to 83.15%, 70.32%, 73.03%, 4.80%, 3.26%, 72.0%, ? 18.88% and 12.58%, respectively. The time of germination, fresh weight and percent moisture contents enhanced insignificantly, however, the response was higher than control. All gamma radiation absorbed doses showed a stimulatory effect on sunflower germination and seedling growth characteristics. The low gamma radiation absorbed doses were found to be more effective versus higher doses for enhancing the germination and growth characteristics of sunflower. In view of positive effect of gamma radiation of sunflower germination and growth characteristics, it is concluded that this techniques could possibly be used for the enhancement of germination, growth and ultimately yield in sunflower in areas where germination is low due to unfavorable conditions. 相似文献
224.
我们在进行新疆地衣分类的过程中, 采用微量化学结晶法(MCT), 薄层色谱法(TLC) 等方法检测地衣物质, 通过观察子囊盘结构和颜色和对地衣次生产物的分析, 并对采自于新疆昭苏卡音德、昭苏木扎特、察布查尔县所采集的蜈蚣衣科(Physciaceae) 400余号标本进行了整理及分类, 发现中国新记录地衣4 种(Phaeophyscia cernohorskyi, Ph decolor,Ph pusilloides, Physconia elegantula), 对此4种新记录地衣进行简要的描述。本文所引用标本均保存于新疆大学资源植物标本室。 相似文献
225.
Tripathi S Somashekar BS Mahdi AA Gupta A Mahdi F Hasan M Roy R Khetrapal CL 《Journal of biochemical and molecular toxicology》2008,22(2):119-127
The toxic effects of Al(3+) have been studied in 90-days AlCl(3) orally treated male albino rats (n = 7) using (1)H NMR spectroscopy-based metabolic profile of rat serum and urine, serum enzyme tests, behavioral impairment, and histopathology of kidney and liver. Metabolic profile of 90-days Al(3+)-treated rat sera showed significantly elevated levels of alanine, glutamine, beta-hydroxy-butyrate, and acetoacetate and significantly decreased level of acetone when compared with that of control rats. However, metabolic profile of 90-days Al(3+)-treated rat urine showed significantly decreased levels of citrate, creatinine, allantoin, trans-aconitate, and succinate and significantly increased level of acetate when compared to control rats. The overall perturbations observed in the metabolic profile of serum and urine demonstrate the impairment in the tricarboxylic acid cycle, liver and kidney metabolism, which was further reinstated by clinical chemistry and histopathological observations. Moreover, "in vivo" behavioral impairment has also been observed as the indication of aluminum neurotoxicity. 相似文献
226.
Down-regulation of DNMT3b in PC3 cells effects locus-specific DNA methylation,and represses cellular growth and migration 总被引:1,自引:0,他引:1
Background
Aberrations in DNA methylation patterns promote changes in gene expression patterns and are invariably associated with neoplasia. DNA methylation is carried out and maintained by several DNA methyltransferases (DNMTs) among which DNMT1 functions as a maintenance methylase while DNMT3a and 3b serve as de novo enzymes. Although DNMT3b has been shown to preferentially target the methylation of DNA sequences residing in pericentric heterochromatin whether it is involved in gene specific methylation remains an open question. To address this issue, we have silenced the expression of DNMT3b in the prostate-derived PC3 cells through RNA interference and subsequently studied the accompanied cellular changes as well as the expression profiles of selected genes. 相似文献227.
Emami S Foroumadi A Falahati M Lotfali E Rajabalian S Ebrahimi SA Farahyar S Shafiee A 《Bioorganic & medicinal chemistry letters》2008,18(1):141-146
2-Hydroxyphenacyl azole and 2-hydroxyphenacyl azolium compounds have been described as a new class of azole antifungals. Most target compounds showed significant in vitro antifungal activities against tested fungi (Candida albicans, Saccharomyces cerevisiae, Aspergillus niger, and Microsporum gypseum) with low MICs values included in the range of 0.25-32 microg/mL comparable to reference drug fluconazole. The most active compounds were also assessed for their cytotoxicity using MTT colorimetric assay on normal mouse fibroblast (NIH/3T3) cells. The results of antifungal activity and toxicity tests indicated that these compounds display antifungal activity at non-cytotoxic concentrations. 相似文献
228.
Ibrahim MA Shilabin AG Prasanna S Jacob M Khan SI Doerksen RJ Hamann MT 《Bioorganic & medicinal chemistry》2008,16(14):6702-6706
Quaternary carbolinium salts have been reported to show improved antimalarial activity and reduced cytotoxicity as compared to electronically neutral beta-carbolines. In this study, mono- and di-methylated quaternary carbolinium cations of manzamine A were synthesized and evaluated for their in vitro antimalarial and antimicrobial activity, cytotoxicity, and also their potential for glycogen synthase kinase (GSK-3beta) inhibition using molecular docking studies. Among the analogs, 2-N-methylmanzamine A (2) exhibited antimalarial activity (IC(50) 0.7-1.0microM) but was less potent than manzamine A. However the compound was significantly less cytotoxic to mammalian kidney fibroblasts and the selectivity index was in the same range as manzamine A. 相似文献
229.
Ghulam Abbas Yanhua Lan George Kostakis Christopher E. Anson Annie K. Powell 《Inorganica chimica acta》2008,361(12-13):3494-3499
A series of six isostructural lanthanide dimers of general formula [Ln2(mdeaH2)2(piv)6], where mdeaH2 is N-methyldiethanolamine, piv is pivalate, and Ln = La, Ce, Pr, Nd, Sm, and Gd, has been surveyed to gauge the nature of the magnetic interactions between the lanthanide centres. Single-crystal X-ray structure analyses indicate that the lanthanides are connected by syn,syn-carboxylate bridges. It was found from an analysis of their bulk magnetic susceptibilities as a function of temperature that this type of bridge mediates vanishingly small magnetic interactions. This finding is important in the context of developing synthetic strategies for the preparation of new molecular based magnetic materials. 相似文献
230.
The discovery of dendritic cells (DCs) as professional antigen presenting cells has opened up new possibilities for their use in the development of tumor vaccines. We investigated the effect of the CD8α+ DCs loaded with heat-treated tumor lysate (HTL) as a vaccine in tumor immunotherapy. The HTL loaded CD8α+ DCs, TL loaded CD8α+ DCs and unloaded CD8α+ DCs were subcutaneously injected in the fibrosarcoma-bearing mice. The splenocyte proliferation and the shifting of Th1/Th2 response were measured. The results indicated a significant increase in the lymphocytes proliferation and the IFN-γ production in the test group of mouse splenocytes. According to the results, HTL loaded CD8α+ DCs vaccine significantly decreased tumor growth and longer survival than the other immunized animals. These findings show that anti-tumor immune response against the fibrosarcoma can be induced by HTL loaded CD8α+ DCs and may provide a useful therapeutic model for development of approaches to tumor treatments. 相似文献