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71.
72.
Sahún I Gallego X Gratacòs M Murtra P Trullás R Maldonado R Estivill X Dierssen M 《Amino acids》2007,33(4):677-688
Summary. Sensitivity to pharmacological challenges has been reported in patients with panic disorder. We have previously validated
transgenic mice overexpressing the neurotrophin-3 (NT-3) receptor, TrkC (TgNTRK3), as an engineered murine model of panic
disorder. We could determine that TgNTRK3 mice presented increased cellularity in brain regions, such as the locus ceruleus,
that are important neural substrates for the expression of anxiety in severe anxiety states. Here, we investigated the sensitivity
to induce anxiety and panic-related symptoms by sodium lactate and the effects of various drugs (the α2-adrenoceptor antagonist,
yohimbine and the adenosine antagonist, caffeine), in TgNTRK3 mice. We found enhanced panicogenic sensitivity to sodium lactate
and an increased intensity and a differential pattern of Fos expression after the administration of yohimbine or caffeine
in TgNTRK3. Our findings validate the relevance of the NT-3/TrkC system to pathological anxiety and raise the possibility
that a specific set of fear-related pathways involved in the processing of anxiety-related information may be differentially
activated in panic disorder. 相似文献
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Simon J. Watson Pinky Langat Scott M. Reid Tommy Tsan-Yuk Lam Matthew Cotten Michael Kelly Kristien Van Reeth Yu Qiu Ga?lle Simon Emilie Bonin Emanuela Foni Chiara Chiapponi Lars Larsen Charlotte Hjulsager Iwona Markowska-Daniel Kinga Urbaniak Ralf Dürrwald Michael Schlegel Anita Huovilainen Irit Davidson ádám Dán Willie Loeffen Stephanie Edwards Michel Bublot Thais Vila Jaime Maldonado Laura Valls ESNIP Consortium Ian H. Brown Oliver G. Pybus Paul Kellam 《Journal of virology》2015,89(19):9920-9931
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Silvas J Prince Li Song Dan Qiu Joao V Maldonado dos Santos Chenglin Chai Trupti Joshi Gunvant Patil Babu Valliyodan Tri D Vuong Mackensie Murphy Konstantinos Krampis Dominic M Tucker Ruslan Biyashev Anne E Dorrance MA Saghai Maroof Dong Xu J Grover Shannon Henry T Nguyen 《BMC genomics》2015,16(1)
Background
Root system architecture is important for water acquisition and nutrient acquisition for all crops. In soybean breeding programs, wild soybean alleles have been used successfully to enhance yield and seed composition traits, but have never been investigated to improve root system architecture. Therefore, in this study, high-density single-feature polymorphic markers and simple sequence repeats were used to map quantitative trait loci (QTLs) governing root system architecture in an inter-specific soybean mapping population developed from a cross between Glycine max and Glycine soja.Results
Wild and cultivated soybean both contributed alleles towards significant additive large effect QTLs on chromosome 6 and 7 for a longer total root length and root distribution, respectively. Epistatic effect QTLs were also identified for taproot length, average diameter, and root distribution. These root traits will influence the water and nutrient uptake in soybean. Two cell division-related genes (D type cyclin and auxin efflux carrier protein) with insertion/deletion variations might contribute to the shorter root phenotypes observed in G. soja compared with cultivated soybean. Based on the location of the QTLs and sequence information from a second G. soja accession, three genes (slow anion channel associated 1 like, Auxin responsive NEDD8-activating complex and peroxidase), each with a non-synonymous single nucleotide polymorphism mutation were identified, which may also contribute to changes in root architecture in the cultivated soybean. In addition, Apoptosis inhibitor 5-like on chromosome 7 and slow anion channel associated 1-like on chromosome 15 had epistatic interactions for taproot length QTLs in soybean.Conclusion
Rare alleles from a G. soja accession are expected to enhance our understanding of the genetic components involved in root architecture traits, and could be combined to improve root system and drought adaptation in soybean.Electronic supplementary material
The online version of this article (doi:10.1186/s12864-015-1334-6) contains supplementary material, which is available to authorized users. 相似文献78.
Maldonado B Buchanan G Müller M Berks BC Palmer T 《Journal of molecular microbiology and biotechnology》2011,20(3):168-175
The twin arginine protein transport (Tat) system transports folded proteins across the cytoplasmic membranes of prokaryotes and the thylakoid membranes of plant chloroplasts. In Escherichia coli, the TatB and TatC components form a multivalent receptor complex that binds Tat substrates. Here, we have used a genetic fusion approach to construct covalent TatC oligomers in order to probe the organisation of TatC. A fused dimer of TatC supported Tat transport activity and was fully stable in vivo. Inactivating point mutations in one or other of the TatC units in the fused TatC dimer did not inactivate TatC function, indicating that only one TatC protomer in the TatC fused dimer needs to be active. Larger covalent fusions of TatC also supported Tat transport activity but were degraded in vivo to release smaller TatC forms. Taken together, these results strongly suggest that TatC forms a functional dimer, and support the idea that there is an even number of TatC protomers in the TatBC complex. 相似文献
79.
Maldonado GE Pérez CA Covarrubias EE Cabriales SA Leyva LA Pérez JC Almaguer DG 《Cytotherapy》2011,13(10):1249-1255
BACKGROUND AIMS. Lymphedema is a common complication with breast cancer treatment that does not have a definite cure. Our objective was to determine the efficacy of autologous stem cells (ASC) in the treatment of lymphedema secondary to mastectomy and axillary lymphadenectomy in comparison with traditional decongestive treatment with compression sleeves. METHODS. A prospective study including 20 women with lymphedema secondary to breast cancer surgery with axillary lymphadenectomy was conducted. Women were assigned at random to one of two groups. One group of 10 women was injected with ASC in the affected arm, whereas the other 10 women comprised the control group and received traditional compression sleeve therapy (CST). The follow-up for both groups was 12 weeks. Pain, sensitivity and mobility were assessed before and after therapy. RESULTS. There was improvement in the volume of lymphedema in both groups, with no significant difference. In the ASC group there was an overall volume reduction during the follow-up, whereas in the CST group lymphedema recurred after the compression sleeve was removed. CONCLUSIONS. Our findings suggest that ASC injection for patients with lymphedema can be an effective treatment. It reduces arm volume and associated co-morbidities of pain and decreased sensitivity. Traditional CST was also effective for lymphedema reduction, but it was dependent on continuous use of the treatment. 相似文献
80.
Gracia P. González-Porter Frank Hailer Oscar Flores-Villela Rony García-Anleu Jesús E. Maldonado 《Conservation Genetics》2011,12(5):1229-1242
We conducted a phylogeographic analysis of the strictly aquatic and critically endangered Central American river turtle, Dermatemys mawii, as part of a conservation management program for the species. We sampled 238 individuals from 15 different localities throughout
the species range. Using sequence fragments from the mtDNA Cyt b and ND4 genes, we identified 16 different haplotypes. Overall, our results reveal a signal of phylogeographic structure throughout
the range, which appears to have been secondarily blurred by extensive gene flow. Notably, this also applies to genetic structuring
across three major hydrological basins that pose biogeographic breaks in other aquatic taxa. Divergence times of mtDNA haplotypes
in D. mawii suggest that the main lineages split in the Pliocene–Pleistocene (3.73–0.227 MA) and demographic tests indicate that the
species has undergone drastic demographic size fluctuations since this time period. One ancient haplotype (1D) was found to
exhibit sequence divergence of up to 2% from other haplogroups. Divergence of this magnitude is indicative of species level
differentiation in other turtle genera. Haplotype 1D was found in only two localities, Sarstun and Salinas, but specimens
with other haplotypes were also found in those localities. It is not known whether the individuals with the 1D haplotype interbreed
with non-1D individuals. Our results suggest that human activity, such as harvesting and long distance transport of animals,
may have influenced the current patterns of genetic diversity. For more than 2000 years, D. mawii has been consumed by people from Middle American cultures, and the archeological record contains strong evidence that the
Mayans transported animals between villages and far away from their natural distribution range. Therefore, the large-scale
pattern of haplotype sharing even across hydrological barriers, the observed low haplotype diversity in some populations and
the contemporary absence of a pronounced phylogeographic pattern is likely due to a combination of population expansions,
gene flow, extensive human-mediated-movements and recent bottlenecks resulting from over-harvesting. 相似文献