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排序方式: 共有211条查询结果,搜索用时 38 毫秒
91.
92.
Audrey Leprince Mark WJ van Passel Vitor AP Martins dos Santos 《Current opinion in biotechnology》2012,23(5):651-658
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93.
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Celia Méndez-García Victoria Mesa Richard R Sprenger Michael Richter María Suárez Diez Jennifer Solano Rafael Bargiela Olga V Golyshina ángel Manteca Juan Luis Ramos José R Gallego Irene Llorente Vitor AP Martins dos Santos Ole N Jensen Ana I Peláez Jesús Sánchez Manuel Ferrer 《The ISME journal》2014,8(6):1259-1274
Macroscopic growths at geographically separated acid mine drainages (AMDs) exhibit distinct populations. Yet, local heterogeneities are poorly understood. To gain novel mechanistic insights into this, we used OMICs tools to profile microbial populations coexisting in a single pyrite gallery AMD (pH ∼2) in three distinct compartments: two from a stratified streamer (uppermost oxic and lowermost anoxic sediment-attached strata) and one from a submerged anoxic non-stratified mat biofilm. The communities colonising pyrite and those in the mature formations appear to be populated by the greatest diversity of bacteria and archaea (including ‘ARMAN'' (archaeal Richmond Mine acidophilic nano-organisms)-related), as compared with the known AMD, with ∼44.9% unclassified sequences. We propose that the thick polymeric matrix may provide a safety shield against the prevailing extreme condition and also a massive carbon source, enabling non-typical acidophiles to develop more easily. Only 1 of 39 species were shared, suggesting a high metabolic heterogeneity in local microenvironments, defined by the O2 concentration, spatial location and biofilm architecture. The suboxic mats, compositionally most similar to each other, are more diverse and active for S, CO2, CH4, fatty acid and lipopolysaccharide metabolism. The oxic stratum of the streamer, displaying a higher diversity of the so-called ‘ARMAN''-related Euryarchaeota, shows a higher expression level of proteins involved in signal transduction, cell growth and N, H2, Fe, aromatic amino acids, sphingolipid and peptidoglycan metabolism. Our study is the first to highlight profound taxonomic and functional shifts in single AMD formations, as well as new microbial species and the importance of H2 in acidic suboxic macroscopic growths. 相似文献
95.
N. L. Eremeev I. V. Nikolaev I. D. Keruchen’ko E. V. Stepanova A. D. Satrutdinov S. V. Zinov’ev D. Yu. Ismailova V. P. Khotchenkov N. V. Tsurikova A. P. Sinitsyn V. G. Volik O. V. Koroleva 《Applied Biochemistry and Microbiology》2009,45(6):648-655
Optimization of the process of enzymatic hydrolysis of keratin-containing stock aimed at obtaining hydrolysates of high biological value has been performed. The increasing of the stock/water weight ratio, the amount of the alkaline protease preparation from Acremonium chrysogenium added and the temperature of the reaction mixture resulted in an increase in the yield and antioxidant capacity of hydrolysis products. The molecular masses of soluble products obtained under optimal hydrolysis conditions ranged from 3.55 to 3.60 kDa. High antioxidant capacity, 100% bioavailability and a well-balanced amino acid composition was characteristic of the hydrolysis products. 相似文献
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The long-term time behavior of the output power of a sealed-off cryogenic slab CO laser pumped by a repetitively pulsed RF discharge and operating on the overtone (λ = 2.6–3.5 μm) vibrational?rotational transitions of the CO molecule was studied experimentally. It is shown that adding of an anomalously large amount of oxygen (up to 50% with respect to the CO concentration) to the initial gas mixture CO : He = 1 : 10 leads to a manyfold (by several tens of times) increase in the duration of the laser operating cycle (until lasing failure due to the degradation of the active medium). In this case, the laser life-time without replacement of the active medium reaches 105–106 pulses. Using various diagnostics (including luminescence spectroscopy and IR and UV absorption spectroscopy), regularities in the time-behavior of the concentrations of the main component of the active medium (CO molecules) and the products of plasmachemical reactions (O3, CO2) generated in the discharge gap during the laser operating cycle are revealed. Time correlation between the characteristics of the active medium and the laser output power are analyzed. A phenomenological approach to describing the entirety of plasmachemical, purely chemical, gas-dynamic, and diffusion processes determining the behavior of the laser output characteristics throughout the laser operating cycle is offered. 相似文献
99.
N.?G.?LoikoEmail author N.?E.?Suzina V.?S.?Soina T.?A.?Smirnova M.?V.?Zubasheva R.?R.?Azizbekyan D.?O.?Sinitsyn K.?B.?Tereshkina Yu.?A.?Nikolaev Yu.?F.?Krupyanskii G.?I.?El’-Registan 《Microbiology》2017,86(6):714-727
Compaction and biocrystallization of the nucleoid are presently considered as a necessary and important stage in the transformation of the cell ultrastructure during change of microbial cultures strategies from growth to survival. Nucleoid biocrystallization in the stationary phase cells is achieved due to structural regularity of the DNA complexes with the histone-like Dps protein. Our experiments with Escherichia coli mutants, overproducers of the Dps protein, confirmed nucleoid biocrystallization in the late stationary phase cells. Since nucleoid biocrystallization was revealed in E. сoli cells without Dps overproduction at late stages of starvation, it is constitutive in the cycle of development of microbial populations. The present work concentrated on detection of the nucleoid biocrystalline structure in (1) long-starved (21 day in the chemostat mode) bacterial cells (genera Arthrobacter and Pseudomonas), (2) dormant ametabolic (anabiotic) cells of such prokaryotes as archaea and non-spore-forming bacteria, (3) endospores of bacilli, (4) streptomycete exospores, and (5) in the cells surviving in permafrost for (2?3 Ma). The topics discussed include nucleoid biocrystallization as a necessary stage of maturation of the dormant microbial cells providing for survival and preservation of the species, dynamics of nucleoid biocrystallization during maturation of the dormant cells, and its possible role for the preservation of genetic information in the case of autolysis of most of the cells in a developing culture. 相似文献
100.
Vidhan Jain Henry B Armah Jon E Tongren Renée M Ned Nana O Wilson Sara Crawford Pradeep K Joel Mrigendra P Singh Avinash C Nagpal AP Dash Venkatachalam Udhayakumar Neeru Singh Jonathan K Stiles 《Malaria journal》2008,7(1):1-15