中国芒果输出蒸热杀虫处理研究

中国芒果输出蒸热杀虫处理研究,包括海南产金煌、爱文、象牙和台农1号4个品种芒果鲜果内橘小实蝇Bactrocera dorsalis(Hendel)的卵和不同龄期幼虫的耐热力试验;芒果小规模、大规模蒸热杀虫处理试验;以及蒸热处理对芒果品质的影响等。结果显示,当蒸热室温度逐步达到50℃,芒果果心温度上升至47℃(使用饱和热蒸汽)并保持这一温度时,在不同处理时间(0、5、10、15、20min)条件下,不同品种芒果内橘小实蝇的耐热力由强至弱的发育期依次为2龄幼虫→3龄幼虫→1龄幼虫→卵,其中以金煌芒果(海南产芒果品种中果体最大,平均重量856g)内2龄幼虫的耐热力最强。完全杀灭海南产金煌芒果内橘小实蝇的卵和幼虫的蒸热条件是果心温度保持在47℃和使用饱和热蒸汽处理20min。金煌芒果的小规模、大规模蒸热杀虫处理试验的结果进一步确认和验证,在上述的蒸热处理条件下,总计数量分别为14000头和35000头最具耐热力的供试橘小实蝇2龄幼虫全部被杀灭(死亡率100%),完全可确保其检疫安全。同时,经蒸热杀虫处理后第7d和第12d的金煌芒果鲜果与对照鲜果品质(包括色泽,以及还原糖、蔗糖、总糖、总酸、维生素C和可溶性固形物的含量)的比较测定数据表明,蒸热温度47℃和使用饱和热蒸汽处理20min,除维生素C的含量有微量损失外,基本可保持鲜果的品质不变。     

盐酸戊乙奎醚治疗脓毒性休克的临床分析

目的:分析盐酸戊乙奎醚治疗脓毒性休克临床疗效.方法:40例脓毒性休克住院患者随机分为对照组(n=20)和盐酸戊乙奎醚治疗组(PH组,n=20)),对照组予山莨菪碱(654-2)治疗;PH组予盐酸戊乙奎醚治疗.观察患者治疗效果及末梢转暖时间,给药后2h、6h、12h时点患者心率、平均动脉压、瞳孔、动脉血氧饱和度、肠鸣音及血乳酸含量变化.结果:PH组、对照组总有效率比较,差异无统计学意义(P0.05).PH组末梢转暖持续时间明显延长,两组差异有统计学意义(P0.05).与治疗前比较,治疗后两组患者血乳酸含量下降、动脉氧饱和度明显改善;PH组心率、平均动脉压、瞳孔、肠鸣音变化差异无统计学意义.两组间比较,PH组血乳酸水平下降更明显,差异有统计学意义(P<0.05),且两组在2h、6h时点心率、瞳孔、肠鸣音变化比较,差异有统计学意义(P<0.05).治疗后,两组的APCACHE Ⅱ评分、SOFA评分较治疗前减少(P<0.05),且PH组评分减少比对照组更明显(P<0.05).结论:盐酸戊乙奎醚可明显改善休克患者微循环,无明显增加心率、抑制肠蠕动副作用,是脓毒性体克患者较理想的血管活性药.     

Evaluation of adverse effects in tamoxifen exposed healthy female dogs

Background

Hypertension and proteinuria are medical complications associated with the multisystemic effects of long-term hypercortisolism in dogs with hyperadrenocorticism (HAC).

Methods

This study investigated the relationships among adrenocorticotropic hormone (ACTH)-stimulation test results, systemic blood pressure, and microalbuminuria in clinically-healthy dogs (n = 100), in dogs affected with naturally occurring pituitary-dependent (PDH; n = 40), or adrenal-dependent hyperadrenocorticism (ADH; n = 30).

Results

Mean systemic blood pressure was similar between clinically healthy dogs and dogs with HAC (p = 0.803). However the incidence of hypertension was highest in dogs with ADH (p = 0.017), followed by dogs with PDH, with the lowest levels in clinically healthy dogs (p = 0.019). Presence of microalbuminuria and albuminuria in clinically healthy dogs and dogs affected with HAC was significantly different (p < 0.001); incidences of albuminuria followed the same pattern of hypertension; highest incidence in dogs with ADH, and lowest level in clinically healthy dogs; but microalbuminuria showed a different pattern: clinically healthy dogs had highest incidences and dogs with ADH had lowest incidence. The presence of albuminuria was not associated with blood pressure values, regardless of whether dogs were clinically healthy or affected with ADH or PDH (p = 0.306).

Conclusions

Higher incidence of hypertension and albuminuria, not microalbuminuria was seen in dogs affected with HAC compared to clinically healthy dogs; incidence of hypertension and albuminuria was significantly higher in dogs affected with ADH compared to PDH. However, presence of albuminuria was not correlated with systemic blood pressure.     

酵母酸性磷酸酯酶转录调控因子PHO2的功能域分析

利用定点诱变,氨基酸插入或缺失的方法对ＰＨＯ２进行结构改造,观察对其功能的影响。ＰＨＯ２蛋白的同源域中有α－螺旋２－β－转我－α螺旋３的结构,是转录因子结构ＤＮＡ的功能域。定点诱变α－螺旋３上的Ｉｌｅ１２３为Ｐｒｏ１２３或者在α－螺旋２中插入ＰＤＰＤ４个氨基酸,破坏α－螺旋的结构,可导致ＰＨＯ２功能的丧失。氨基酸片段的缺失分析表明,Ｎ端Ｇｉｎ丰富区大部缺失,对ＰＨＯ２功能没有影响;而包含酸性氨基酸     

ELISA法检测流行性腮腺炎病毒感染特异性IgM抗体的研究

用流行性腮腺炎(流腮)病毒Enders株接种鸡胚尿囊腔培养,尿囊液经聚乙二醇6000处理制备流腮病毒抗原,用ELISA法检测流腮患者血清中特异性IgM抗体,其敏感性,特异性、重复性和稳定性都很高。 79份流腮患者血清,检出特异性IgM72份,阳性率为91％,32例非流腮患者IgM全部阴性、两者有极显著差异(P<0.01)。 10份血清作血清倍比稀释至1∶3200测IgM仍全部阳性,1∶6400稀释仅1例阴性,1∶12800稀释5例中仍有2例阳性。 10份血清作流腮抗原特异性抗体阻断试验,光密度抑制率均大于50％,平均为87％,10份标本作2-ME和SPA阻断后检测IgM抗体,结果2-ME阻断标本全部阴转,而SPA阻断标本仍阳性,证实所检测为流腮特异性抗体。 24份标本2次重复检测流腮IgM,其阴、阳性结果一致,这期间抗原放4℃ 1个月,提示抗原的稳定性和方法的重复性都很好。本方法敏感性明显高于血凝抑制试验,其阳性率分别为91％和61％,两者有显著差异。而且所用试剂简单经济,操作简便,快速,适用于临床早期诊断,易于广泛推广应用。     

单细胞微凝胶电泳技术在人血淋巴细胞DNA损伤研究中的应用

介绍了单细胞微凝胶电泳(SCG)技术的原理和操作过程,并应用该技术研究了γ-线照射、过氧化氢(H_2O_2)、氯化镉(CdCl_2)对人血淋巴细胞DNA的损伤效应。结果表明,γ-线照射、H_2O_2和CdCl_2均能引起细胞DNA迁移长度增加,且呈显著的剂量效应关系。对未处理对照细胞DNA迁移的原因及SCG实验操作过程中应注意的事项也进行了讨论。     

酵母PHO2与PHO4蛋白的激活活性的分析及两者的相互作用

ＰＨＯ２与ＰＨＯ４是酵母ＰＨＯ５基因的两个正调控因子,本文发现,ＰＨＯ２与酵母转录因子ＧＡＬ４的ＤＮＡ结合功能域融合后就能激活报道基因ｌａｃＺ的表达,其激活力受高低磷影响,表明ＰＨＯ２蛋白上存在酸性转录激活区。ＰＨＯ２蛋白上酸性氨基酸丰富的２８７－３２６肽段并非ＰＨＯ２的激活区。在ＰＨＯ２蛋白上２３０位Ｓｅｒ处于磷酸化状态２ＰＨＯ２才有激活作用,表明了这一磷酸化位点可能与ＰＨＯ２的转录激活能力有关     

酵母PHO4基因的克隆与表达

用原位杂交方法从酵母菌染色体ＤＮＡ中分段克隆,拼接,获得完整的ＰＨＯ４基因,全长约３．４ｋｂ,利用体内同源重组的方法,构建了ＰＨＯ４基因缺失突变株。ＰＨＯ４基因的缺失导致酸性磷酸酯酶活性明显下降,将完整的ＰＨＯ４基因转入这种缺陷细胞,能使酶活性回复到野生型水平,ＰＨＯ４起正调控作用,构建ＰＨＯ４－ＬａｃＺ融合基因,以β－半乳糖苷酶的活力表示ＰＨＯ４的表达水平。融合基因不同名的表达表明,ＰＨＯ４基因     

Assembly of the sarcoplasmic reticulum proteins in differentiating rat skeletal muscle cell cultures: localization by immunofluorescence of sarcoplasmic reticulum proteins in differentiating rat skeletal muscle cell cultures

Immunofluorescent staining techniques were used to study the distribution of the Ca(2) + Mg(2+)-dependent ATPase and calsequestrin in primary cultures of differentiating rat skeletal muscle cells, grown for different periods of time under various culture conditions. In mononucleated myoblasts calsequestrin was detected after 45 h in culture whereas the ATPase was not detected until 60 h. After cell fusion began, both proteins could be identified in all multinucleated cells. Myoblasts grown for longer than 60 h in low Ca(2+) medium contained calsequestrin and the ATPase, even though they were unable to fuse. These studies at the cellular level confirm biochemical findings on the biosynthesis of calsequestrin and the ATPase. Immunofluorescent staining of myoblasts showed that calsequestrin first appears in a well-defined region of the cell near one end of the nucleus. At later times, the staining occupied progressively larger regions adjacent to the nucleus and took on a fibrous appearance. This suggests that calsequestrin first accumulates in the Golgi region and then gradually spreads throughout the cell. In contrast, the ATPase appeared to be concentrated in many small patches or foci throughout the cytoplasm and was never confined to one particular region, although some parts of the cell often stained more intensely than others. In multinucleated cells, alternating dark and fluorescent strands parallel to the longitudinal axis of the cells were evident.