Dieback and mortality of South African fynbos shrubs is likely driven by a novel pathogen and pathogen‐induced hydraulic failure

We examined whether extensive dry season dieback and mortality in a South African fynbos community were due to drought or pathogen attack. Plant dieback and mortality have been reported elsewhere in similar plant communities suggesting potential for a widespread climatic or biotic threat to this community. We collected tissue samples from Brunia noduliflora, the dominant plant in the community, and cultured them for potential plant pathogens. We also measured dry season predawn and midday water potentials of healthy and stressed plants and constructed pressure‐volume curves to assess turgor loss point. Plant stress and mortality were monitored over a 2‐year study period. Both healthy plants and plants that displayed moderate signs of stress had dry season predawn water potentials well above their turgor loss point suggesting plants were not water stressed. However, plants displaying >60% crown dieback had much lower water potentials (as low as ?12 MPa). A previously undescribed fungus (Pythium sp.) was isolated from the root vascular tissue of all stressed plants and was not present in healthy plants. The proximate cause of plant stress was likely pathogen‐induced, while the ultimate cause of plant death appears to be extreme water stress. The present study suggests that Brunia (Bruniceae), Leucadendron (Proteaceae) and Erica (Ericaceae), all emblematic and dominant genera within the diverse fynbos community, may be susceptible to Pythium infection. This may pose a serious threat to communities already threatened by climate change.     

Rapid In Vitro Prescreen for Chelators as Potential Trypanocides Based on Growth of Crithidia fasciculata1

A rapid in vitro prescreen for Fe-binding chelators has been developed with growth of Crithidia fasciculata and the sparing of its heme requirement in a defined medium as a test system. The prescreen functions as an index of chelator-mediated Fe transport and as an index of growth inhibition, presumably by the interference with Fe and/or heme metabolism at intracellular chelatable sites. Of 161 chelators examined, 84 were active heme-sparers; 32 of these inhibited growth at low chelator concentrations. Twenty-eight other chelators inhibited growth and another 49 were inactive. Such chelating activity directed at Fe and heme targets in hemoflagellates may provide leads for chemotherapy.     

Edge Effect on Density Estimates of a Radiotracked Population of Yellow-Necked Mice

ABSTRACT Density estimates for small-mammal populations from capture-mark-recapture (CMR) data have played an important role in many studies of theoretical and applied ecology. Defining effective trapping area (ETA) is one of the main issues affecting accuracy of density estimates. Our objective was to assess sensitivity of CMR density estimates to correctors based on movement parameters calculated from trapping and radiotelemetry data. From May to November 2005, we conducted monthly CMR trapping in a beech (Fagus sylvaticus) forest of the province of Trento, northern Italy. In conjunction with CMR, we radio-marked 32 yellow-necked mice (Apodemus flavicollis) captured from July to October and located them daily using radiotelemetry. We estimated population size (N) by model averaging with Program MARK. We calculated ETA using several definitions of the boundary strip, including full and half mean maximum distance moved (MMDM) from capture-recapture and telemetry data and mean radius of mean monthly home ranges. The boundary strip (W) increased with the amount of behavioral information embodied in the estimates. The largest W and lowest density values were based on radius of mean home ranges followed by MMDM calculated from telemetry data. The ETA based on movement distances increased more than proportionally when N decreased, suggesting that low population density combined with scarce resources results in rodents moving more in search of food, thus leading to overestimated ETA and underestimated density values. Although robust behavioral information would certainly improve density estimates, we suggest caution in relating ranging movements to capture probability and hence in using correctors based on movement distances to infer density values.     

Lipoic Acid Does Not Affect The Growth of Mycoplasma hominis Cells In Vitro

Mycoplasma hominis is associated with various infections, for which the treatment can be complex. Lipoic acid (LA) plays a role as a cofactor in eukaryotes, most Bacteria, and some Archea. Research of recent years has increasingly pointed to the therapeutic properties of exogenously supplemented LA. The present study was conducted on 40 strains of M. hominis cultured with the following LA concentrations: 1,200 μg/ml, 120 μg/ml, and 12 μg/ml. The bacterial colonies of each strain were counted and expressed as the number of colony-forming units/ml (CFU). The number of CFU in M. hominis strains obtained in the presence of LA was compared with the number of CFU in the strains grown in the media without LA. The obtained results indicated that the presence of LA in the medium did not affect the growth of M. hominis. The investigation of the influence of LA on the growth and survival of microbial cells not only allows for obtaining an answer to the question of whether LA has antimicrobial activity and, therefore, can be used as a drug supporting the treatment of patients infected with a given pathogenic microorganism. Such studies are also crucial for a better understanding of LA metabolism in the microbial cells, which is also important for the search for new antimicrobial drugs. This research is, therefore, an introduction to such further studies.