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91.
92.
RICHARD BISCHOF ANDREAS ZEDROSSER SVEN BRUNBERG JON E. SWENSON 《The Journal of wildlife management》2009,73(6):1021-1024
ABSTRACT Out of precaution, opportunism, and a general tendency towards thoroughness, researchers studying wildlife often collect multiple, sometimes highly correlated measurements or samples. Although such redundancy has its benefits in terms of quality control, increased resolution, and unforeseen future utility, it also comes at a cost if animal welfare (e.g., duration of handling) or time and resource limitation are a concern. Using principle components analysis and bootstrapping, we analyzed sets of morphometric measurements collected on 171 brown bears in Sweden during a long-term monitoring study (1984–2006). We show that of 11 measurements, 7 were so similar in terms of their predictive power for an overall size index that each individual measurement provided little additional information. We argue that when multiple research objectives or data collection goals compete for a limited amount of time or resources, it is advisable to critically evaluate the amount of additional information contributed by extra measurements. We recommend that wildlife researchers look critically at the data they collect not just in terms of quality but also in terms of need. 相似文献
93.
Abstract. Forty-two enzyme-substrate systems were tested on starch gels in order to characterize the Ephemeroptera biochemically. Of these, twelve systems were useful and enabled the evaluation of sixteen loci. This biochemical method correlated well with the results of the morphological characterization of the four European Epeorus taxa, which are subdivided into two groups: E.sylvicola-E.torrentium and E.alpicola-E.yougoslavicus. Our biochemical comparison with the type species of Iron (I.longimanus) from North America clearly shows that all four European taxa belong to the genus Epeorus and that Iron is a distinct genus. Keys to larvae and imagines are provided. 相似文献
94.
SANJA BARIC ANDREAS RIEDL ANDREAS MERANER NIKOLAUS MEDGYESY REINHARD LACKNER BERND PELSTER JOSEF DALLA VIA 《Freshwater Biology》2010,55(4):866-880
1. The watercourses of Austria are mainly part of the Danube drainage system. Nonetheless, only few brown trout (Salmo trutta) populations of the Danubian phylogenetic lineage have been found in this region, not being introgressed by hatchery‐reared fish of the allochthonous Atlantic lineage. The present study was aimed at identifying further waterbodies that might harbour the non‐admixed gene pool of the Danubian clade trout. 2. In a first step, the complete mitochondrial DNA control region of 447 specimens from 20 sampling sites was sequenced to screen for the presence of specific haplotypes. In a second step, 332 individuals from 15 populations were analysed at 10 microsatellite DNA loci. 3. Although sampling concentrated on remote Alpine headwater streams, introgression of hatchery strains was more common than expected from existing stocking records. For seven populations, however, no evidence of recent hatchery trout introgression was found and it is suggested that they might represent the indigenous gene pool. 4. Populations showing no evidence of hatchery introgression displayed lower levels of intrapopulation variability and higher degrees of differentiation compared to purported admixed populations. 5. Our study demonstrates the necessity of combining different molecular markers to reveal the impact of introgression into wild populations, since at some sampling sites admixture would have remained undetected if only a single molecular technique was applied. 6. The identification and characterisation of non‐introgressed populations provides important information for setting conservation priorities and preventing further loss of genetic integrity of a unique element of the native fish fauna. 相似文献
95.
Components of partial resistance of barley to Rhynchosporium secalis: use of seedling tests to predict field resistance 总被引:1,自引:0,他引:1
Components of partial resistance [disease incidence (DI), infection frequency (IF), latent period (LP), spores per lesion (SPL)] were assessed on glasshouse-grown barley seedlings following inoculation with spore suspensions of Rhynchosporium secalis at growth stage 12 (Zadoks, Chang & Konzak, 1974). Four experiments were carried out at different times during 1988. Three spring barley cultivars [two from Cyprus (cvs Kantara and Athenais) and one from the UK (cv. Triumph)] were used in the first three experiments. In the fourth experiment eight additional UK cultivars with NIAB resistance ratings ranging from 3 to 9 were used. Two races of R. secalis were used in the first three experiments and three in the fourth. The three cultivars, Kantara, Athenais and Triumph, were examined in all four experiments and significant differences detected for virtually all components of partial resistance in each. Differences, however, were often small and ranking of cultivars varied in different experiments. The greater susceptibility of cv. Kantara compared to cv. Athenais, observed under field conditions in Cyprus, would not be anticipated from the small differences in components of partial resistance observed in these experiments, but, for these cultivars, the possibility of a marked genotype x environment interaction cannot be discounted. Mean values for the components of partial resistance differed in the four experiments. LP was correlated with mean glasshouse temperature from inoculation to the onset of sporulation but differences in IF and SPL were not correlated with temperature. For these components, light quality and/or duration appeared to be more important. Overall, there were no differences between races but significant race X cultivar interactions were observed in two experiments. In the fourth experiment, examining 11 cultivars, there were significant differences between cultivars for all components of partial resistance. IF and LP were correlated but neither of these components was correlated with SPL indicating independent control of this latter component. Both IF and LP were correlated with field performance (NIAB ratings) but there was no correlation with SPL. However, combining IF with mean values of SPL restricted to the 5 days following the end of the LP, produced the best correlation (r= 0.92) with NIAB ratings. Problems of assessing components of partial resistance and possible means of improving assessments are discussed. 相似文献
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97.
CHRISTOPH ANDREAS LEHMEIER FERNANDO ALFREDO LATTANZI RUDI SCHÄUFELE & HANS SCHNYDER 《Plant, cell & environment》2010,33(1):76-87
Plant respiration draws on substrate pools of different functional/biochemical identity. Little is known about the effect of nitrogen deficiency on those pools' sizes, half-lives and relative contribution to respiration, and consequently, of carbon residence time in respiratory metabolism. Here we studied how nitrogen fertilization affects the respiratory carbon supply system of shoots and roots of Lolium perenne , a perennial grass. Plants grown at two nitrogen supply levels in continuous light were labelled with 13 CO2 /12 CO2 for intervals ranging from 1 h to 1 month. The rate and isotopic composition of shoot, root and plant respiration were measured, and the time-courses of tracer incorporation into respired CO2 were analysed by compartmental modelling. Nitrogen deficiency reduced specific respiration rate by 30%, but increased the size of the respiratory supply system by 30%. In consequence, mean residence time of respiratory carbon increased with nitrogen deficiency (4.6 d at high nitrogen and 9.2 d at low nitrogen supply). To a large extent, this was due to a greater involvement of stores with a long half-life in respiratory carbon metabolism of nitrogen-deficient plants. At both nitrogen supply levels, stores supplying root respiration were primarily located in the shoot, probably in the form of fructans. 相似文献
98.
Six spring barley cultivars with no known genes for resistance to specific virulences but varying in partial resistance to Rhynchosporium secalis, were crossed in all combinations (6 × 6 diallel including reciprocals). In addition to seeds from naturally selfed plants, seeds of all parent cultivars were also produced by artificial selfing (emasculation followed by pollination using pollen from the same cultivar). This ensured comparability between seeds of parents and F1. Both sets of parents, F1 and F2 families were grown in the field as single spaced plants and inoculated at Zadoks growth stage 49 with spore suspensions (2 × 106 spores ml-1) of three races (pathotypes) of R. secalis (Zadoks, Chang & Konzak, 1974). Components of partial resistance, incubation period (ICP), infection frequency (IF) and spore production per lesion (SP/L) were assessed on each plant. There were highly significant differences for all three components of partial resistance in both sets of parent cultivars but rank order in both sets was similar as evidenced by correlation coefficients, r= 0.96 for ICP and IF and r= 0.87 for SP/L. All three components of partial resistance were strongly correlated with NIAB (National Institute of Agricultural Botany, Cambridge, UK) resistance ratings. Means of F1 and F2 families were correlated with mid-parent values for ICP and IF but not SP/L. No difference in aggressiveness was found between races but for each component of partial resistance there was a significant interaction between race and parent cultivar (artificial selfs) and, for IF and ICP, a significant interaction between race and F1 family. There was no evidence of interaction between parent (natural selfs) and race nor between race and F2 family. Examination of genetic control of resistance showed evidence of strong additive effects (combining ability) in both F1 and F2 for ICP and IF but not for SP/L. There was no evidence for maternal or reciprocal differences, but there was evidence for dominance effects although their nature differed between components of partial resistance and between F1 and F2 generations. In the F1, but not the F2 generation, several elements of dominance (direction, distribution of dominant genes between parent cultivars, specific combining ability) showed for ICP or IF (but not SP/L) significant interaction with race. 相似文献
99.
100.
Genome‐wide association mapping and biochemical markers reveal that seed ageing and longevity are intricately affected by genetic background and developmental and environmental conditions in barley 下载免费PDF全文
MANUELA NAGEL ILSE KRANNER KERSTIN NEUMANN HARDY ROLLETSCHEK CHARLOTTE E. SEAL LOUISE COLVILLE BEATRIZ FERNÁNDEZ‐MARÍN ANDREAS BÖRNER 《Plant, cell & environment》2015,38(6):1011-1022
Globally, over 7.4 million accessions of crop seeds are stored in gene banks, and conservation of genotypic variation is pivotal for breeding. We combined genetic and biochemical approaches to obtain a broad overview of factors that influence seed storability and ageing in barley (Hordeum vulgare). Seeds from a germplasm collection of 175 genotypes from four continents grown in field plots with different nutrient supply were subjected to two artificial ageing regimes. Genome‐wide association mapping revealed 107 marker trait associations, and hence, genotypic effects on seed ageing. Abiotic and biotic stresses were found to affect seed longevity. To address aspects of abiotic, including oxidative, stress, two major antioxidant groups were analysed. No correlation was found between seed deterioration and the lipid‐soluble tocochromanols, nor with oil, starch and protein contents. Conversely, the water‐soluble glutathione and related thiols were converted to disulphides, indicating a strong shift towards more oxidizing intracellular conditions, in seeds subjected to long‐term dry storage at two temperatures or to two artificial ageing treatments. The data suggest that intracellular pH and (bio)chemical processes leading to seed deterioration were influenced by the type of ageing or storage. Moreover, seed response to ageing or storage treatment appears to be significantly influenced by both maternal environment and genetic background. 相似文献