NEW OCTOPODS (CEPHALOPODA: COLEOIDEA) FROM THE LATE CRETACEOUS (UPPER CENOMANIAN) OF HÂKEL AND HÂDJOULA, LEBANON

Abstract:  Three previously unknown octopods are described from Upper Cenomanian limestones of the Hâqel and Hâdjoula localities (Lebanon). Keuppia levante gen. nov., sp. nov., Keuppia hyperbolaris gen. nov,. sp. nov. and Styletoctopus annae gen. nov, . sp. nov. are regarded as the earliest representatives of the Octopoda (= Incirrata). This assumption is mainly based on their medially isolated bipartite gladius vestige. As can be inferred from growth increments, Keuppia gen. nov. can be distinguished from the genus Palaeoctopus by blades that grow forwards along their longitudinal axis. The gladius vestige of Keuppia hyperbolaris sp. nov. differs from that of Keuppia levante sp. nov. in having a more heterogeneous course of growth lines. Based on a pair of widely separated stylets, which closely resemble the rods of modern octopods, Styletoctopus annae gen. nov., sp. nov. is assigned to the Recent family Octopodidae. Peculiar encrustations, which are situated in close association with the gladius vestiges of Keuppia levante sp. nov., Keuppia hyperbolaris sp. nov., and Styletoctopus annae sp. nov. are interpreted as basal fin cartilages. The gladius vestige morphology of Keuppia hyperbolaris sp. nov. and Keuppia levante sp. nov. opens the possibility that both the Octopda and the Cirroctopoda originated from loligosepiid vampyropods instead of teudopseid. The surprising existence of a stylet-like gladius vestige in Styletoctopus annae sp. nov. suggests that the octopod clade branched off much earlier than previously believed. Octopod apomorphies such as the development of stylets, loss of fins and cirri must have been occurred before the Cenomanian.     

Effects of aquatic macrophytes on organic matter deposition,resuspension and phosphorus entrainment in a lowland river

1. Although macrophytes play a key role in the structure and functioning of lowland rivers, most of the basic plant, hydrodynamic and sediment‐water interactions have only been described qualitatively. We therefore studied quantitatively, the seasonal dynamics of matter deposition and mobilisation inside and outside (free path) a representative patch of arrowhead, Sagittaria sagittifolia, in the lowland River Spree, NE Germany, in August 2006. Our in situ study combined resuspension experiments, a hydrodynamically calibrated erosion chamber and concurrent measurements of the prevailing flow characteristics and bed load. 2. Increasing entrainment rates (E) of particles (E_SPM) and total P (E_TP), with increments of shear velocity (U_*) from 0.53 to 2.42 cm s^?1, were significantly higher inside the plant patch than outside. Indeed, E_SPM and E_TP at the lowest U_* were 8‐ and 12‐fold higher inside than outside the patch, reflecting the resuspension potential of the upper nutrient‐enriched layer and the extent of pulsed P inputs even at small increases in U_*. 3. Vertical distribution of velocity (u) revealed a flow pattern of a mixing layer inside the S. sagittifolia patch, and that of a boundary layer in the free path. The highest gradient of u in the mixing layer was located in the water column at about 0.5 m depth, whereas the highest gradient of u for the boundary layer was found near the riverbed. The maximum of U_* (1.65 cm s^?1) was only 4 mm above the sediment. 4. A plant mosaic provides a low‐energetic environment promoting extensive particle trapping and the accumulation of a fine‐grained, nutrient‐enriched sediment, and forming a large resuspension potential. Consequently, after plant decay and the concomitant increase of U_* this material is preferentially entrained at higher rates. Hence, the key role of submerged macrophytes in lowland rivers is more directly related to modifying the dynamic equilibria between vegetation trapping and resuspension, than to the retention of nutrients, particularly P, and the reduction of P loads downstream to other waters.     

Fast response of Scots pine to improved water availability reflected in tree‐ring width and δ13C

Drought‐induced forest decline, like the Scots pine mortality in inner‐Alpine valleys, will gain in importance as the frequency and severity of drought events are expected to increase. To understand how chronic drought affects tree growth and tree‐ring δ¹³C values, we studied mature Scots pine in an irrigation experiment in an inner‐Alpine valley. Tree growth and isotope analyses were carried out at the annual and seasonal scale. At the seasonal scale, maximum δ¹³C values were measured after the hottest and driest period of the year, and were associated with decreasing growth rates. Inter‐annual δ¹³C values in early‐ and latewood showed a strong correlation with annual climatic conditions and an immediate decrease as a response to irrigation. This indicates a tight coupling between wood formation and the freshly produced assimilates for trees exposed to chronic drought. This rapid appearance of the isotopic signal is a strong indication for an immediate and direct transfer of newly synthesized assimilates for biomass production. The fast appearance and the distinct isotopic signal suggest a low availability of old stored carbohydrates. If this was a sign for C‐storage depletion, an increasing mortality could be expected when stressors increase the need for carbohydrate for defence, repair or regeneration.     

Wing‐beat characteristics of birds recorded with tracking radar and cine camera

This study presents wing‐beat frequency data measured mainly by radar, complemented by video and cinematic recordings, for 153 western Palaearctic and two African species. Data on a further 45 Palaearctic species from other sources are provided in an electronic appendix. For 41 species with passerine‐type flight, the duration of flapping and pausing phases is given. The graphical presentations of frequency ranges and wing‐beat patterns show within‐species variation and allow easy comparison between species, taxonomic groups and types of flight. Wing‐beat frequency is described by Pennycuick (J. Exp. Biol. 2001; 204: 3283–3294) as a function of body‐mass, wing‐span, wing‐area, gravity and air density; for birds with passerine‐type flight the power‐fraction has also to be considered. We tested Pennycuick’s general allometric model and estimated the coefficients based on our data. The general model explained a high proportion of variation in wing‐beat frequency and the coefficients differed only slightly from Pennycuick’s original values. Modelling continuous‐flapping flyers alone resulted in coefficients not different from those predicted (within 95% intervals). Doing so for passerine‐type birds resulted in a model with non‐significant contributions of body‐mass and wing‐span to the model. This was mainly due to the very high correlation between body‐mass, wing‐span and wing‐area, revealing similar relative scaling properties within this flight type. However, wing‐beat frequency increased less than expected with respect to power‐fraction, indicating that the drop in flight level during the non‐flapping phases, compensated by the factor (g/q)^0.5 in Pennycuick’s model, is smaller than presumed. This may be due to lift produced by the body during the bounding phase or by only partial folding of the wings.     

Selection of male traits during and after copulation in the seedbug Lygaeus simulans (Heteroptera, Lygaeidae)

In order to evaluate selection of male morphological traits during copulation, a laboratory experiment was performed with the promiscuous seedbug Lygaeus simulans. Three male traits suspected as putative targets of selection were measured: weight, fluctuating asymmetry of a measure on the forewings, and length of a conspicuous genital structure, the processus gonopori. As fitness measures we considered total fecundity (number of fertilized eggs), insemination and fertilization success (established if a female laid fertilized eggs after copulation), and the interval between copulation and oviposition. Eighty-four males were allowed a single copulation with one virgin female each. Out of 67 copulations, 27 (40.2%) resulted in fertilized eggs and the oviposition latency ranged from 6 to 26 days. Regressions of male traits on the fitness measures showed significant phenotypical selection of two male traits: (1) males of average weight are more likely to achieve fertilization and (2) the oviposition latency was shorter for males with lower asymmetry. The copulation-oviposition interval may be especially important for male fertilization success because Lygaeus males perform copulatory mate guarding and the last male copulating with a female fertilizes most of the eggs. No selection of the genitalic trait was detected.     

Importance of colour in the reaction of passerine predators to aposematic prey: experiments with mutants of Pyrrhocoris apterus (Heteroptera)

Persistent questions concerning the warning coloration of unpalatable insects address whether the bright aposematic colour itself or its combination with a species-specific dark pattern is the key factor in their protection against insectivorous birds, and how chromatic polymorphism originates and is maintained in aposematics. In the present study, these questions were tested experimentally, using the birds Parus major , Parus caeruleus , Erithacus rubecula , and Sylvia atricapilla as predators, and chromatically polymorphic firebug Pyrrhocoris apterus : red wild form, white, yellow, and orange mutants (all four of them with the same black melanin pattern, the mutants differing in colour of pteridine pigments only) and the nonaposematic brown-painted wild form as prey. The results show that a specific colour is essential for the birds to recognize the specific aposematic prey; the melanin pattern is not sufficient. White mutants were no better protected than nonaposematic firebugs; red wild-type and orange mutants were equally well protected against all bird species; and the reaction of birds to yellow mutants was species-specific. An evolutionary scenario of 'recurrent recessive mutations' is formulated to explain the origin of colour polymorphism in some aposematics.  © 2006 The Linnean Society of London, Biological Journal of the Linnean Society , 2006, 88 , 143–153.     

Immunological Detection of Cytoskeletal Proteins In the Exoerythrocytic Stages of Malaria By Fluorescence and Confocal Laser Scanning Microscopy

ABSTRACT. Using monospecific antibodies, the presence and distribution of tubulin, actin, myosin, intermediate filaments, and lamins were examined in the exoerythrocytic liver schizont of Plasmodium berghei by conventional indirect fluorescent antibody methods and confocal laser scanning microscopy. the binding reactivity of the antibodies to parasite proteins was determined by Western blot analysis. the localisation of all antibodies in control host hepatocytes followed expected distributions in both uninfected and infected hepatocytes; by contrast, reactivity to the exoerythrocytic schizont was variable. the parasite reacted positively with selected anti-tubulin, -actin, and -myosin antibodies in both fluorescence and Western blot analysis. Anti-lamin antibodies were positive by confocal indirect fluorescent antibody labelling, but no labelling was detected with anti-intermediate filament antibody. Within the technical limits of resolution of the methods as applied to asynchronous parasite infections, not one of the antibodies reacting positively with the parasite by the indirect fluorescent antibody technique could be shown to identify unequivocally the classic architectural features associated with their respective target organelles, i.e. microtubules, stress-fibres or the nuclear envelope.     

中国淡脉隧蜂属指名亚属三新种(膜翅目:蜜蜂总科, 隧蜂科)

本文记述中国淡脉隧蜂属指名亚属Lasioglossum(Lasioglossum)三新种。所有正模、配模和大多数副模标本保存于中国科学院动物研究所,少量副模标本保存于奥地利国P. Andreas Werner Ebmer处。     

Pollination of the edelweiss, Leontopodium alpinum

This paper describes the pollinators, scent and nectar composition of the edelweiss ( Leontopodium alpinum). In contrast to former statements in the literature, the flowers of this famous plant are frequently visited by various insects in 29 familieS. However, in the two populations of L. Alpinum investigated here, flies (Muscidae) were the most frequent visitorS. Although the investigated insects carried pollen grains all over their bodies and qualify as mess and soil pollinators, pollen grains were not randomly distributed on the bodies of the insects but were most concentrated on their legs. Their loads of L. Alpinum pollen varied greatly, but more than 60% of all Leontopodium pollen grains found on the insects were carried by only 12% of the specimens collected, suggesting that a small fraction of the visitors is responsible for most of the pollen transfer. The number of pollen grains found on the insects also increased significantly with increasing body size of the insects. The scent of the flowers is sweet and honey-like (phenylethyl alcohol and phenylacetic acid) but also contains disagreeably smelling, sweat-like components (3-methyl-2-pentenoic acids, butyric acid and other fatty acids).Nectar is secreted in minute amounts in the tiny florets of the flower heads. The sugar composition of the nectar is dominated by the hexoses fructose and glucose. It also contains a high amino acid concentration with many different amino acidS. Nectar, scent and the shape of the flowers of L. Alpinum all fit the syndrome of fly pollination.