Food plant and herbivore host species affect the outcome of intrinsic competition among parasitoid larvae

1. In nature, several parasitoid species often exploit the same stages of a common herbivore host species and are able to coexist despite competitive interactions amongst them. Less is known about the direct effects of resource quality on intrinsic interactions between immature parasitoid stages. The present study is based on the hypothesis that variation in the quality or type of plant resources on which the parasitoids indirectly develop may be complementary and thus facilitate niche segregation favouring different parasitoids in intrinsic competition under different dietary regimes. 2. The present study investigated whether two herbivore species, the cabbage butterflies Pieris brassicae and Pieris rapae (Pieridae), and the quality of two important food plants, Brassica oleracea and Brassica nigra (Brassicaceae), affect the outcome of intrinsic competition between their primary larval endoparasitoids, the gregarious Cotesia glomerata (Braconidae) and the solitary Hyposoter ebeninus (Ichneumonidae). 3. Hyposoter ebeninus is generally an intrinsically superior competitor over C. glomerata. However, C. glomerata survived more antagonistic encounters with H. ebeninus when both developed in P. brassicae rather than in P. rapae caterpillars, and while its host was feeding on B. nigra rather than B. oleracea. Moreover, H. ebeninus benefitted from competition by its higher survival in multiparasitised hosts. 4. These results show that both plant and herbivore species mediate the battleground on which competitive interactions between parasitoids are played out and may affect the outcomes of these interactions in ways that enable parasitoids to segregate their niches. This in turn may promote coexistence among parasitoid species that are associated with the same herbivore host.     

Dry nitrogen deposition estimates over a forest experiencing free air CO2 enrichment

The quantification of atmospheric additions of nitrogen (N) to an ecosystem is often desirable, but difficult for many locations including many ecological manipulation experiments. Ideal methodologies for the complete and chemically speciated quantification of dry N deposition (e.g. tunable diode laser absorption spectrometer, thermal‐dissociation laser‐induced fluorescence) are expensive, technologically challenging to maintain, and rarely colocated with important global change manipulation experiments. Here we present an alternative method for obtaining an approximation of total N deposition using short‐term eddy flux and concentration measurements, annual regional concentration estimates, and modeling for the Duke Experimental Forest. The motivation for generating estimates for this location was to inform the long‐term elevated CO₂ experiment conducted at Duke Forest. We estimated the total annual atmospheric N deposition to the forest to be 13.7 kg N ha^?1. Of this total, ～58% was in dry‐deposited forms. Surprisingly, and contrary to some previous predictions, nitric acid (HNO₃) was not the dominant portion of the total dry‐deposited N, implying strongly that other forms of gaseous N like organic peroxy and alkyl nitrate compounds are a significant portion of the total flux. Furthermore, this study sheds light on the completeness of the estimates derived from Clean Air Status and Trends Network (CASTNet) and other dry deposition networks. CASTNet does not measure organic forms of dry deposition. In fact, CASTNet only quantifies HNO₃ in the gas phase and may significantly underestimate total N deposition in many environments.     

Amphibian chytridiomycosis in Japan: distribution,haplotypes and possible route of entry into Japan

A serious disease of amphibians caused by the chytrid fungus Batrachochytrium dendrobatidis was first found in Japan in December 2006 in imported pet frogs. This was the first report of chytridiomycosis in Asia. To assess the risk of pandemic chytridiomycosis to Japanese frogs, we surveyed the distribution of the fungus among captive and wild frog populations. We established a nested PCR assay that uses two pairs of PCR primers to amplify the internal transcribed spacer (ITS) region of a ribosomal RNA cassette to detect mild fungal infections from as little as 0.001 pg (1 fg) of B. dendrobatidis DNA. We collected swab samples from 265 amphibians sold at pet shops, 294 bred at institutes and 2103 collected at field sites from northern to southwestern Japan. We detected infections in native and exotic species, both in captivity and in the field. Sequencing of PCR products revealed 26 haplotypes of the B. dendrobatidis ITS region. Phylogenetic analysis showed that three of these haplotypes were specific to the Japanese giant salamander (Andrias japonicus) and appeared to have established a commensal relationship with this native amphibian. Many other haplotypes were carried by alien amphibians. The highest genetic diversity of B. dendrobatidis was found in the American bullfrog (Rana catesbeiana). Some strains of B. dendrobatidis appeared to be endemic to Japanese native amphibians, but many alien strains are being introduced into Japan via imported amphibians. To improve chytridiomycosis risk management, we must consider the risk of B. dendrobatidis changing hosts as a result of anthropogenic disturbance of the host‐specific distribution of the fungus.     

Using multitemporal digital elevation model data for detecting canopy gaps in tropical forests due to cyclone damage: An initial assessment

Abstract The detection and mapping of canopy disturbance caused by natural events such as cyclones in tropical rainforests is important for monitoring and understanding rainforest dynamics and recovery. In February 1999 tropical cyclone Rona crossed the coastline of Far North Queensland, Australia, causing significant damage to the rainforest canopy in some areas. This paper examines the application of multitemporal canopy digital elevation models (DEMs) generated by a NASA‐operated airborne radar mapping system called TOPSAR in 1996 and 2000, for detection of canopy disturbance caused by cyclone damage. Canopy damage was mapped here by identifying areas with a significant decrease in canopy height estimated from the difference of the 1996 and 2000 TOPSAR DEMs. Conventional aerial photographs, flown shortly after the cyclone (March 1999), were used to validate the resulting map of cyclone damaged rainforest canopy. The results showed that the DEM‐derived canopy damage map performed reasonably well when comparing the spatial distribution and size of damaged areas, while taking into account the time difference between cyclone damage and the second radar acquisition. Some errors were encountered on the steeper slopes which were related to terrain distortions inherent in radar images of steep terrain. The height accuracy of the DEM was close to the depth of some of the gaps being mapped, which also contributed to errors. Even so, the results demonstrate there may be potential for weather‐independent, regional‐scale mapping of forest canopy change from imaging radar that is not always possible from traditional optical means of measuring canopy elevation, for example, airborne laser data and stereo aerial photography. This may be possible in relatively flat areas, provided the second radar acquisition occurs within months of the damaging event. Further algorithm refinement is required to improve its robustness and the range of topographies where this approach can provide reliable estimates of the extent of canopy disturbance.     

The influence of expanding leaves and the reproductive stem apex on apical dominance in Lolium multiflorum

In vegetative plants of Lolium multiflorum removal of the two youngest emerging leaves resulted in increased expansion of basal tiller buds. A similar release of inhibition of tiller buds took place if the floriferous apex was removed. The surgical procedures did not affect the response. Under conditions of N-deficiency total tiller number was reduced but on removal of the apex the deficient plants showed an increased initial rate of tiller bud expansion. Apical dominance during the vegetative stage of growth in this grass was apparently due to the expanding leaves in the vegetative apex, but in the flowering plant the control was exerted by the inflorescence or the elongating stem.     

Observations on Some Internal Structures and Pseudopods of Lesquereusia spiralis (Rhizopoda, Testacida)

SYNOPSIS. The internal structure and activities of Lesquereusia spiralis was observed by the use of nonmotile individuals which had incorporated a cover glass as part of their shell. As many as 14 ectoplasmic strands or epipods connect the main cytoplasmic mass to the inner surface of the shell. Two to 14 contractile vacuoles 2–5 μ in diameter at 22–25 C were present. A few epipods contained contractile vacuoles. The vacuoles emptied every 1–2 min. Both the number of epipods and contractile vacuoles varied with the degree of pseudopod activity; the more active the pseudopods, the more numerous these organelles. Egestion of indigestible food residues from the cytoplasm takes place just beyond the mouth of the shell thru vacuoles about 15 μ in diameter. Frequently, long flattened pseudopods of a tensile or contracting type were formed. In some cases pseudopods of this type were seen to pull themselves in 2 when the distal end remained attached and contraction continued.     

Non‐trophic effects of oribatid mites on cord‐forming basidiomycetes in soil microcosms

1. Saprotrophic cord‐forming basidiomycetes are the primary agents of decomposition in forest ecosystems. Collembola and oribatid mites affect fungal growth and foraging, and therefore decomposition, through direct mycelial grazing. 2. Grazing on the fungal species Hypholoma fasciculare, Resinicium bicolor and Phanerochaete velutina by the collembola Folsomia candida, and the oribatid mites Steganacarus magnus, Euzetes globulus and Hermannia gibba was investigated in soil microcosms. 3. Folsomia candida grazed on all fungal species: radial extent of R. bicolor, hyphal coverage of all fungal species, and fractal dimension of R. bicolor and P. velutina were all reduced. Oribatid mites did not graze the fungi but did affect mycelial morphology. Steganacarus magnus caused a reduction in the radial extent of H. fasciculare, and the hyphal coverage and fractal dimension in both H. fasciculare and R. bicolor. Euzetes globulus and H. gibba reduced the hyphal coverage of P. velutina. 4. Oribatid mites are associated with a cornucopia of chemical secretions with possible anti‐fungal properties. Chemical analysis of H. gibba opisthonotal secretions revealed four main compounds, all of which are new to the known spectrum of opisthonotal components. The most abundant was (E)‐β‐farnesene. 5. Treatment effects were species‐specific in terms of both fungal and invertebrate species. This study provides the first evidence of non‐grazing effects of oribatid mites on fungal growth and morphology. This could potentially influence the spatial organisation of mycelium in forest soils and therefore the ability of fungi to locate, colonise and decompose dead organic matter.     

Stramenopile Microorganisms Associated with the Massive Coral Favia sp.

ABSTRACT. The surfaces of massive corals of the genus Favia from Eilat, Red Sea, and from Heron Island, Great Barrier Reef, are covered by a layer of eukaryotic microorganisms. These microorganisms are embedded in the coral mucus and tissue. In the Gulf of Eilat, the prevalence of corals covered by patches of eukaryotic microorganisms was positively correlated with a decrease in water temperatures (from 25–28 °C in the summer to 20–23 °C in winter). Comparisons carried out using transmission and scanning electron microscopy showed morphological similarities between the microorganisms from the two geographically distant reefs. The microorganisms found on and in the tissues were approximately 5–15 μm in diameter, surrounded by scales in their cell wall, contained a nucleus, and included unique auto‐florescent coccoid bodies of approximately 1 μm. Such morphological characters suggested that these microorganisms are stramenopile protists and in particular thraustochytrids. Molecular analysis, carried out using specific primers for stramenopile 18S rRNA genes, revealed that 90% (111/123) of the clones in the gene libraries were from the Thraustochytriidae. The dominant genera in this family were Aplanochytrium sp., Thraustochytrium sp., and Labyrinthuloides sp. Ten stramenopile strains were isolated and cultured from the corals. Some strains showed ≥97% similarity to clones derived from libraries of mucus‐associated microorganisms retrieved directly from these corals. Fatty acid characterization of one of the prevalent strains revealed a high percentage of polyunsaturated fatty acids, including omega‐3. The possible association of these stramenopiles in the coral holobiont appeared to be a positive one.