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81.
Laurin AJ Mueller Karl G Kugler Michael Netzer Armin Graber Matthias Dehmer 《Biology direct》2011,6(1):1-15
Background
Chromosomal orthologs can reveal the shared ancestral gene set and their evolutionary trends. Additionally, physico-chemical properties of encoded proteins could provide information about functional adaptation and ecological niche requirements.Results
We analyzed 7080 genes (five groups of 1416 orthologs each) from Rhizobiales species (S. meliloti, R. etli, and M. loti, plant symbionts; A. tumefaciens, a plant pathogen; and B. melitensis, an animal pathogen). We evaluated their phylogenetic relationships and observed three main topologies. The first, with closer association of R. etli to A. tumefaciens; the second with R. etli closer to S. meliloti; and the third with A. tumefaciens and S. meliloti as the closest pair. This was not unusual, given the close relatedness of these three species. We calculated the synonymous (dS) and nonsynonymous (dN) substitution rates of these orthologs, and found that informational and metabolic functions showed relatively low dN rates; in contrast, genes from hypothetical functions and cellular processes showed high dN rates. An alternative measure of sequence variability, percentage of changes by species, was used to evaluate the most specific proportion of amino acid residues from alignments. When dN was compared with that measure a high correlation was obtained, revealing that much of evolutive information was extracted with the percentage of changes by species at the amino acid level. By analyzing the sequence variability of orthologs with a set of five properties (polarity, electrostatic charge, formation of secondary structures, molecular volume, and amino acid composition), we found that physico-chemical characteristics of proteins correlated with specific functional roles, and association of species did not follow their typical phylogeny, probably reflecting more adaptation to their life styles and niche preferences. In addition, orthologs with low dN rates had residues with more positive values of polarity, volume and electrostatic charge.Conclusions
These findings revealed that even when orthologs perform the same function in each genomic background, their sequences reveal important evolutionary tendencies and differences related to adaptation. This article was reviewed by: Dr. Purificación López-García, Prof. Jeffrey Townsend (nominated by Dr. J. Peter Gogarten), and Ms. Olga Kamneva. 相似文献82.
Alexandra Esther J��rgen Groeneveld Neal J. Enright Ben P. Miller Byron B. Lamont George L. W. Perry Britta Tietjen Florian Jeltsch 《Theoretical Ecology》2011,4(4):495-511
Mathematical models and ecological theory suggest that low-dimensional life history trade-offs (i.e. negative correlation
between two life history traits such as competition vs. colonisation) may potentially explain the maintenance of species diversity
and community structure. In the absence of trade-offs, we would expect communities to be dominated by ‘super-types’ characterised
by mainly positive trait expressions. However, it has proven difficult to find strong empirical evidence for such trade-offs
in species-rich communities. We developed a spatially explicit, rule-based and individual-based stochastic model to explore
the importance of low-dimensional trade-offs. This model simulates the community dynamics of 288 virtual plant functional
types (PFTs), each of which is described by seven life history traits. We consider trait combinations that fit into the trade-off
concept, as well as super-types with little or no energy constraints or resource limitations, and weak PFTs, which do not
exploit resources efficiently. The model is parameterised using data from a fire-prone, species-rich Mediterranean-type shrubland
in southwestern Australia. We performed an exclusion experiment, where we sequentially removed the strongest PFT in the simulation
and studied the remaining communities. We analysed the impact of traits on performance of PFTs in the exclusion experiment
with standard and boosted regression trees. Regression tree analysis of the simulation results showed that the trade-off concept
is necessary for PFT viability in the case of weak trait expression combinations such as low seed production or small seeds.
However, species richness and diversity can be high despite the presence of super-types. Furthermore, the exclusion of super-types
does not necessarily lead to a large increase in PFT richness and diversity. We conclude that low-dimensional trade-offs do
not provide explanations for multi-species co-existence contrary to the prediction of many conceptual models. 相似文献
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84.
Egg strings and larvae of Hochstetter's frog (Leiopelma hochstetteri) were located at three widely separated North Island sites: in seeps at Brynderwyns in December 2004, in an open pool at Wharerino in March 2009, and in an underground pool near the Kaipawa Track, Coromandel, in late May 2009. Ten egg strings were also laid by captive frogs in water courses at Hamilton Zoo in April 2009. All egg strings held from 11 to 13 eggs. The egg strings laid in the Brynderwyns were regularly observed until metamorphosis was completed in March 2005. Twenty-four swimming larvae emerged from 25 capsules at c. 40 days after discovery, and at least 14 froglets were produced at c. 90 days. All of them developed in darkness, in a 120 ml pool <30 mm deep. The emerged froglets ranged from 9.8 to 10.8 mm snout-vent length. The detection of eggs, larvae and <11 mm froglets indicates that the egg laying period is at least from late September to May. 相似文献
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87.
The impact of a trichloroethylene (TCE) contaminated wastewater on the microbial community structure of an anaerobic granular biomass at 15 °C compared to 37 °C was investigated. Four expanded granular sludge bed (EGSB) bioreactors (R1-R4) were employed in pairs at 37 and 15 °C. The influents of one of each pair were supplemented with increasing concentrations of TCE (max. 60 mg l−1). At 37 °C, stable operation was maintained with 88% COD removal and >99% TCE removal at maximum influent TCE concentrations. R3 performance decreased at influent TCE concentration of 60 mg l−1, although TCE removal rates of >97% were recorded. Archaeal community analysis via clone library and quantitative polymerase chain reaction (qPCR) analysis, and bacterial community analysis via denaturing gradient gel electrophoresis (DGGE), indicated that temperature resulted in a greater change in community structure than the presence of TCE, and clones related to cold adaptation of biomass were identified at 15 °C. 相似文献
88.
Lin Liu Edward Enright Peng Sun Shwu Yar Tsai Pragna Mehta David L Beckman David M Terrian 《European journal of biochemistry》2002,269(17):4277-4286
We investigated the effect of nitric oxide (NO) donors on the activities of annexin II tetramer (AIIt), a member of the Ca2+- dependent phospholipid-binding protein family. Incubation of purified AIIt with S-nitrosoglutathione (GSNO) led to the inhibition of AIIt-mediated liposome aggregation. This effect was dose-dependent with an IC50 of approximately 100 micro m. Sodium nitroprusside, another NO donor also inhibited AIIt-mediated liposome aggregation, whereas reduced glutathione, nitrate, or nitrite had no effects. GSNO also inhibited AIIt-mediated membrane fusion, but not the binding of AIIt to the membrane. GSNO only has a modest effect on liposome aggregation mediated by annexins I, III or IV. The binding of AIIt to the membrane protected the reactive sites of GSNO on AIIt. GSNO did not inhibit AIIt-mediated liposome aggregation in the presence of dithiothreitol. Taken together, our results suggest that GSNO inactivates AIIt possibly via S-nitrosylation and/or the formation of disulfide bonds. 相似文献
89.