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Sizuo Matsuoka Jesus Ferro Paulo Arruda 《In vitro cellular & developmental biology. Plant》2009,45(3):372-381
Biomass has gained prominence in the last few years as one of the most important renewable energy sources. In Brazil, a sugarcane
ethanol program called ProAlcohol was designed to supply the liquid gasoline substitution and has been running for the last
30 yr. The federal government’s establishment of ProAlcohol in 1975 created the grounds for the development of a sugarcane
industry that currently is one of the most efficient systems for the conversion of photosynthate into different forms of energy.
Improvement of industrial processes along with strong sugarcane breeding programs brought technologies that currently support
a cropland of 7 million hectares of sugarcane with an average yield of 75 tons/ha. From the beginning of ProAlcohol to the
present time, ethanol yield has grown from 2,500 to around 7,000 l/ha. New technologies for energy production from crushed
sugarcane stalk are currently supplying 15% of the electricity needs of the country. Projections show that sugarcane could
supply over 30% of Brazil’s energy needs by 2020. In this review, we briefly describe some historic facts of the ethanol industry,
the role of sugarcane breeding, and the prospects of sugarcane biotechnology 相似文献
494.
Carla Sousa-Santos H. F. Gante J. Robalo P. Proença Cunha A. Martins M. Arruda M. J. Alves V. Almada 《Conservation Genetics》2014,15(3):665-677
The use of molecular techniques has shown that hybridization and introgression have significant impacts in evolution, by means of transfer of genetic variation and formation of hybrid species. In this paper we use mitochondrial and nuclear sequence data to investigate the evolutionary history, levels of genetic diversity and population differentiation of a rare and endangered fish species. Our results suggest that a hybrid origin scenario of Chondrostoma olisiponensis is a likely explanation for the shared genetic and morphological traits with Iberochondrostoma and Achondrostoma + Pseudochondrostoma. The basal positioning of C. olisiponensis alleles in all loci analyzed indicates that hybridization events occurred before differentiation within each of these groups, most likely during Middle–Late Miocene. Originally described as C. olisiponensis, we suggest that this species should be placed in the genus Iberochondrostoma to avoid confusion with ‘real’ central European Chondrostoma and to (partially) reflect its evolutionary history. Analyses of levels of genetic diversity and patterns of population subdivision show that populations of the rare Iberochondrostoma olisiponensis are differentiated (high and significant φST and F ST) and genetically depauperate (very low S, π, and θ). I. olisiponensis is simultaneously imperiled by small population sizes and contemporary bidirectional hybridization with another critically endangered sympatric species (Iberochondrostoma lusitanicum). Urgent ex-situ conservation measures involving supportive breeding of I. olisiponensis are needed to preserve present genetic variation and eventually increase in situ population sizes, along with further studies focused on different life history and behavioral characteristics of this highly endangered species. 相似文献
495.
D Kniaz J A Arruda 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1991,196(4):444-450
We studied the mechanism of adaptation to metabolic alkalosis by the turtle urinary bladder in vitro. Turtles were made alkalotic by administration of oral NaHCO3. Bladders removed from alkalotic turtles had an increased rate of HCO3- secretion in vitro as compared with that of control. H+ secretion, however, was not different, indicating that metabolic alkalosis selectively increases HCO3- secretion. Fluorescence microscopy was used to quantify the carbonic anhydrase cells. The total number of carbonic anhydrase cells was determined by mucosal staining of the bladder with 6-carboxyfluorescein diacetate. The number of HCO3(-)-secreting cells (beta cells) was quantified by mucosal staining with NBD-taurine and the number of H(+)-secreting cells (alpha cells) was calculated from the difference between the two. Metabolic alkalosis significantly increased the total number of 6-carboxyfluorescein positive cells and NBD-taurine-positive cells. The increase in the number of 6-carboxyfluorescein positive cells was totally accounted for by the increase in the NBD-taurine-positive cells without change in the number of alpha cells. If NBD-taurine accurately reflects the number of beta cells, these studies show that the adaptation to metabolic alkalosis is mediated, at least in part, by an increase in the number of HCO3(-)-secreting (beta) cells. 相似文献
496.