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161.
Singh AK Patel J Litbarg NO Gudehithlu KP Sethupathi P Arruda JA Dunea G 《Cell and tissue research》2008,332(1):81-88
When rat omentum becomes activated by intraperitoneal injection of inert polydextran particles, these particles are rapidly
surrounded by cells that express markers of adult stem cells (SDF–1α, CXCR4, WT–1) and of embryonic pluripotent cells (Oct–4,
Nanog, SSEA–1). We have cultured such cells, because they may offer a convenient source of adult stem cells, and have found
that they retain stem cell markers and produce high levels of vascular endothelial growth factor for up to ten passages. After
systemic or local injection of these cultured cells into rats with acute injury of various organs, the cells specifically
engraft at the injured sites. Thus, our experiments show that omental stromal cells can be cultured from activated omentum,
and that these cells exhibit stem cell properties enabling them to be used for repair and possibly for the regeneration of
damaged tissues. 相似文献
162.
Murakami MT Lourenzoni MR Arruda EZ Tomaz MA Viçoti MM Abrego JR Melo PA Arni RK 《Protein and peptide letters》2008,15(9):1002-1008
Bothropstoxin-II a calcium-dependent enzyme from Bothrops jararacussu venom causes tissue damage and several haemostatic disorders including platelet aggregation. In order to elucidate the structural determinants of its multiple pharmacological activities, we have studied the effects of suramin on Bothropstoxin-II and present details concerning the mode of binding. 相似文献
163.
Cytogenetic and random amplified polymorphic DNA analyses carried out in the species Leptodactylus podicipinus, L. ocellatus, L. labyrinthicus, and L. fuscus from rural and urban habitats of the northwest region of S?o Paulo State, Brazil, showed that the karyotypes (2n = 22), constitutive heterochromatin distribution and nucleolus organizer region (NOR) location did not differ between the populations from the two environments. The in situ hybridization with an rDNA probe confirmed the location of the NORs on chromosome 8 revealing an in tandem duplication of that region in one of the chromosomes of L. fuscus. DAPI showed that part of the C-band-positive heterochromatin is rich in AT, including that in the proximity the NORs in L. podicipinus and L. ocellatus. The molecular analyses showed that the two populations (urban and rural) of L. podicipinus and L. fuscus are similar from a genetic point of view. The urban and rural populations of species L. ocellatus and L. labyrinthicus showed differences in genetic structures, probably due to urbanization which interferes with the dispersion of those frogs. The marked differences observed between the two populations of L. ocellatus can be representing the cryptic condition of the species. Unweighted pair-group method of analysis and genetic distance analysis detected the genetic proximity between L. ocellatus and L. fuscus. The results indicate that there was no reduction in the genetic diversity in the populations from the urban environment; however, the survival of these frogs would not be guaranteed in the case of an increase in human impact especially for populations of L. labyrinthicus and L. ocellatus. 相似文献
164.
Campani Gilson Gonçalves da Silva Gabriel Zangirolami Teresa Cristina Perencin de Arruda Ribeiro Marcelo 《Bioprocess and biosystems engineering》2017,40(11):1621-1633
Bioprocess and Biosystems Engineering - Temperature influences the rates of oxygen transfer (OTR) and uptake (q O2) in aerobic bioprocesses. Hence, joint analysis of q O2 and OTR at variable... 相似文献
165.
166.
167.
Rômulo Farias Carneiro Renato Cézar Farias Torres Renata Pinheiro Chaves Mayron Alves de Vasconcelos Bruno Lopes de Sousa André Castelo Rodrigues Goveia Francisco Vassiliepe Arruda Maria Nágila Carneiro Matos Helena Matthews-Cascon Valder Nogueira Freire Edson Holanda Teixeira Celso Shiniti Nagano Alexandre Holanda Sampaio 《Marine biotechnology (New York, N.Y.)》2017,19(1):49-64
A new lectin from Aplysia dactylomela eggs (ADEL) was isolated by affinity chromatography on HCl-activated Sepharose? media. Hemagglutination caused by ADEL was inhibited by several galactosides, mainly galacturonic acid (Ka = 6.05 × 106 M?1). The primary structure of ADEL consists of 217 residues, including 11 half-cystines involved in five intrachain and one interchain disulfide bond, resulting in a molecular mass of 57,228 ± 2 Da, as determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. ADEL showed high similarity with lectins isolated from Aplysia eggs, but not with other known lectins, indicating that these lectins could be grouped into a new family of animal lectins. Three glycosylation sites were found in its polypeptide backbone. Data from peptide-N-glycosidase F digestion and MS suggest that all oligosaccharides attached to ADEL are high in mannose. The secondary structure of ADEL is predominantly β-sheet, and its tertiary structure is sensitive to the presence of ligands, as observed by CD. A 3D structure model of ADEL was created and shows two domains connected by a short loop. Domain A is composed of a flat three-stranded and a curved five-stranded β-sheet, while domain B presents a flat three-stranded and a curved four-stranded β-sheet. Molecular docking revealed favorable binding energies for interactions between lectin and galacturonic acid, lactose, galactosamine, and galactose. Moreover, ADEL was able to agglutinate and inhibit biofilm formation of Staphylococcus aureus, suggesting that this lectin may be a potential alternative to conventional use of antimicrobial agents in the treatment of infections caused by Staphylococcal biofilms. 相似文献
168.
Gustavo N. Marta Fernando F. de Arruda Fabiana A. Miranda Alice R.N.S. Silva Wellington F.P. Neves-Junior Anselmo Mancini Samir A. Hanna Carlos E.C.V. Abreu Joo Luis F. da Silva Jose Eduardo V. Nascimento Cecília Maria K. Haddad Fabio Y. Moraes Rafael Gadia 《Reports of Practical Oncology and Radiotherapy》2021,26(5):756
BackgroundThis study aims to assess the clinical outcomes of patients with spine metastases who underwent stereotactic ablative radiation therapy (SABR) as part of their treatment. SABR has arisen as a contemporary treatment option for spinal metastasis patients with good prognoses.Materials and methodsBetween November 2010 and September 2018, Spinal SABR was performed in patients with metastatic disease in different settings: radical (SABR only), postoperative (after decompression and/or fixation surgery), and reirradiation. Local control (LC), pain control, overall survival (OS) and toxicities were reported.ResultsEighty-five patients (corresponding to 96 treatments) with spine metastases were included. The median age was 59 years (range, 23–91). In most SA BR (82.3%, n = 79) was performed as the first local spine treatment, while in 12 settings (12.5%), fixation and/or decompression surgery was performed prior to SABR. Two-year overall survival rate was 74.1%, and median survival was 19 months. The LC rate at 2 years was 72.3%. With regard to pain control, among 67 patients presenting with pain before SA BR, 83.3% had a complete response, 12.1% had a partial response, and 4.6% had progression. Vertebral compression fractures occurred in 10 patients (11.7%), of which 5 cases occurred in the reirradiation setting. Radiculopathy and myelopathy were not observed. No grade III or IV toxicities were seen.ConclusionThis is the first study presenting a Brazilian experience with spinal SA BR, and the results confirm its feasibility and safety. SABR was shown to produce good local and pain control rates with low rates of adverse events. 相似文献
169.
Grape bagasse generated in the wine production process was characterized through X-ray diffractometry, Fourier transform infrared spectroscopy, scanning electron microscopy, nuclear magnetic resonance and thermogravimetric analysis. The efficiency of this natural material for Cd(II) and Pb(II) adsorption was evaluated using a batch adsorption technique. Factors affecting metal adsorption such as pH and contact time were investigated. Maximum adsorption was found to occur at pH 7.0 and 3.0 for Cd(II) and Pb(II), respectively, and a contact time of 5min was required to reach equilibrium for both metals. With these conditions, adsorption studies were performed using a single solution. In addition, to calculate the adsorption capacities for each metal, the Langmüir isotherm model was used. The adsorption capacities were found to be 0.479 and 0.204mmolg(-1) for Cd(II) and Pb(II), respectively. The results showed that grape bagasse could be employed as a low-cost alternative adsorbent for effluent treatment. 相似文献
170.
J A Arruda L F Gutierrez N A Kurtzman 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1975,149(1):50-55
Acetazolamide, chlorothiazide, ethacrynic acid and furosemide were infused to glucose loaded dogs. Glucose reasorption was unchanged after acetazolamide and chlorothiazide. Ethacrynic acid depressed glucose reabsorption significantly. Furosemide caused a modest decrease in glucose reabsorption which was not significant when the control values were compared with the last three experimental values, but which was significant when all experimental values are analyzed. These diuretics most likely depress glucose reabsorption by acting on the proximal tubule, though a distal effect cannot be excluded. 相似文献