全文获取类型
收费全文 | 448篇 |
免费 | 29篇 |
出版年
2022年 | 3篇 |
2021年 | 9篇 |
2020年 | 8篇 |
2019年 | 7篇 |
2016年 | 8篇 |
2015年 | 14篇 |
2014年 | 16篇 |
2013年 | 19篇 |
2012年 | 19篇 |
2011年 | 29篇 |
2010年 | 12篇 |
2009年 | 13篇 |
2008年 | 15篇 |
2007年 | 18篇 |
2006年 | 23篇 |
2005年 | 17篇 |
2004年 | 21篇 |
2003年 | 19篇 |
2002年 | 11篇 |
2001年 | 11篇 |
2000年 | 16篇 |
1999年 | 5篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 7篇 |
1995年 | 4篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1991年 | 7篇 |
1990年 | 4篇 |
1989年 | 7篇 |
1988年 | 4篇 |
1987年 | 10篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1983年 | 5篇 |
1978年 | 6篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1972年 | 6篇 |
1971年 | 17篇 |
1970年 | 9篇 |
1969年 | 3篇 |
1968年 | 8篇 |
1967年 | 5篇 |
1966年 | 3篇 |
1964年 | 3篇 |
1933年 | 2篇 |
排序方式: 共有477条查询结果,搜索用时 15 毫秒
61.
Expression and localization of P-glycoprotein in human heart: effects of cardiomyopathy. 总被引:7,自引:0,他引:7
Konrad Meissner Bernhard Sperker Christiane Karsten Henriette Meyer Zu Schwabedissen Ute Seeland Michael B?hm Sandra Bien Peter Dazert Christiane Kunert-Keil Silke Vogelgesang Rolf Warzok Werner Siegmund Ingolf Cascorbi Michael Wendt Heyo K Kroemer 《The journal of histochemistry and cytochemistry》2002,50(10):1351-1356
ABC-type transport proteins, such as P-glycoprotein (P-gp), modify intracellular concentrations of many substrate compounds. They serve as functional barriers against entry of xenobiotics (e.g., in the gut or the blood-brain barrier) or contribute to drug excretion. Expression of transport proteins in the heart could be an important factor modifying cardiac concentrations of drugs known to be transported by P-gp (e.g., beta-blockers, cardiac glycosides, doxorubicin). We therefore investigated the expression and localization of P-gp in human heart. Samples from 15 human hearts (left ventricle; five non-failing, five dilated cardiomyopathy, and five ischemic cardiomyopathy) were analyzed for expression of P-gp using real-time RT-PCR, immunohistochemistry, and in situ hybridization. Immunohistochemistry revealed expression of P-gp in endothelium of both arterioles and capillaries of all heart samples. Although P-gp mRNA was detected in all samples, its expression level was significantly reduced in patients with dilated cardiomyopathy. We describe variable expression of P-gp in human heart and its localization in the endothelial wall. Thus, intracardiac concentrations of various compounds may be modified, depending on the individual P-gp level. 相似文献
62.
63.
64.
65.
66.
Ernst Wendt 《Cell and tissue research》1961,53(2):172-184
Ohne ZusammenfassungDie Untersuchungen wurden mit Mitteln des Bundesministers für Atomkernenergie und Wasserwirtschaft durchgeführt. 相似文献
67.
68.
69.
70.
Andreas Daiber Markus Bachschmid Cengiz Kavaklí Daniel Frein Maria Wendt Volker Ullrich Thomas Munzel 《Nitric oxide》2003,9(1):44-52
The present study shows that when freezing nitrite containing biological samples in the presence of sodium and phosphate, a process of tyrosine nitration and S-nitrosocysteine formation is observed. The underlying mechanism is obviously based on the already described pH decrease in sodium phosphate buffered solutions during the freezing process and probably involves nitrous acid as an intermediate. However, in pure potassium phosphate buffer freeze-artefacts were absent. The yield of 3-nitrotyrosine from albumin-bound or free tyrosine depends not only on the concentration of nitrite, tyrosine or protein, and sodium phosphate but also on the velocity of the freezing process. Nitrite and nitrate were quantified by the Griess/nitrate reductase assay. 3-nitrotyrosine formation was quantitatively measured by HPLC analysis with optical and electrochemical detection as well as qualitatively investigated by immunohistochemistry and slot blot analysis using 3-nitrotyrosine specific antibodies. The formation of S-nitrosocysteine was detected by S-nitrosothiol specific antibodies and quantified by a fluorometric assay. Irrespective of the mechanism and although the here presented results cannot be generalized, the data warrant caution for the analysis of nitration or nitros(yl)ation products following freezing of nitrite containing biological material. 相似文献