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271.
Tania Escalante Juan J. Morrone Gerardo Rodríguez‐Tapia 《Biological journal of the Linnean Society. Linnean Society of London》2013,110(3):485-499
Since the 19th Century, two regions have been recognized for North American mammals, which overlap in Mexico. The Nearctic region corresponds to the northern areas and the Neotropical region corresponds to the southern ones. There are no recent regionalizations for these regions under the criterion of endemism. In the present study, we integrate two methods to regionalize North America, using species distribution models of mammals: endemicity analysis (EA) and parsimony analysis of endemicity (PAE). EA was used to obtain areas of endemism and PAE was used to hierarchize them. We found 76 consensus areas from 329 sets classified in 146 cladograms, and the strict consensus cladogram shows a basal polytomy with 14 areas and 16 clades. The final regionalization recognizes two regions (Nearctic and Neotropical) and a transition zone (Mexican Transition Zone), six subregions (Canadian, Alleghanian, Californian‐Rocky Mountain, Pacific Central America, Mexican Gulf‐Central America, and Central America), two dominions (Californian and Rocky Mountain), and 23 provinces. Our analysis show that North America is probably more complex than previously assumed. © 2013 The Linnean Society of London, Biological Journal of the Linnean Society, 2013, 110 , 485–499. 相似文献
272.
Chemotaxis to cAMP and slug migration in Dictyostelium both depend on migA, a BTB protein.
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Chemotaxis in natural aggregation territories and in a chamber with an imposed gradient of cyclic AMP (cAMP) was found to be defective in a mutant strain of Dictyostelium discoideum that forms slugs unable to migrate. This strain was selected from a population of cells mutagenized by random insertion of plasmids facilitated by introduction of restriction enzyme (a method termed restriction enzyme-mediated integration). We picked this strain because it formed small misshapen fruiting bodies. After isolation of portions of the gene as regions flanking the inserted plasmid, we were able to regenerate the original genetic defect in a fresh host and show that it is responsible for the developmental defects. Transformation of this recapitulated mutant strain with a construct carrying the full-length migA gene and its upstream regulatory region rescued the defects. The sequence of the full-length gene revealed that it encodes a novel protein with a BTB domain near the N terminus that may be involved in protein-protein interactions. The migA gene is expressed at low levels in all cells during aggregation and then appears to be restricted to prestalk cells as a consequence of rapid turnover in prespore cells. Although migA- cells have a dramatically reduced chemotactic index to cAMP and an abnormal pattern of aggregation in natural waves of cAMP, they are completely normal in size, shape, and ability to translocate in the absence of any chemotactic signal. They respond behaviorally to the rapid addition of high levels of cAMP in a manner indicative of intact circuitry connecting receptor occupancy to restructuring of the cytoskeleton. Actin polymerization in response to cAMP is also normal in the mutant cells. The defects at both the aggregation and slug stage are cell autonomous. The MigA protein therefore is necessary for efficiently assessing chemical gradients, and its absence results in defective chemotaxis and slug migration. 相似文献
273.
274.
Luis O. Lucifora Alberto L. Cione Roberto C. Menni Alicia H. Escalante 《Ecography》2003,26(5):567-572
Geographic variation in tooth row counts among sand tiger sharks Carcharias taurus (Chondrichthyes), from the SW Atlantic, NW Atlantic and the East China Sea is analyzed in this paper. We found significant differences between sand tigers from the SW Atlantic (Southern Hemisphere population) and each of the other two (Northern Hemisphere) regions in the number of upper lateral tooth rows, and between individuals from the SW Atlantic and the East China Sea in the total number of upper tooth rows. Sand tiger sharks from the two Northern Hemisphere populations did not differ in any of the studied variables. Our results agree with comparisons of vertebral counts between sand tiger sharks from Southern and Northern Hemispheres. Both lines of evidence suggest that Southern and Northern Hemisphere populations of C. taurus were isolated to a larger extent than populations of the Northern Hemisphere. The fossil record of the genus Carcharias begins in the Early Cretaceous and C. taurus is certainly known since the Late Miocene. During the Miocene, the Tethys Sea separating northern and southern land masses was still present and it provided a continuous temperate shallow sea that could allow dispersal of sand tiger sharks along Northern Hemisphere seas. Independent observations on the distribution and evolutionary history of the genera Myripristis , Neoniphon , Sargocentron and Aphanius , and genetic studies on the temperate shark genus Mustelus that indicate a close relationship between the Indo-Pacific M. manazo and the Mediterranean M. asterias suggest that this hypothesis is plausible and deserves to be tested. 相似文献
275.
276.
Characterization of a site-specific restriction endonuclease SphI from Streptomyces phaeochromogenes
A new type-II restriction endonuclease SphI, has been partially purified from Streptomyces phaeochromogenes. SphI recognizes the hexanucleotide sequence 5′-GCATG↓C and cleaves it at the position marked by the arrow. This nucleotide sequence is present twice in SV40 DNA, four times in λ DNA and only once in the cloning vehicles pBR322, pBR325, pBR327 and pBR328. 相似文献
277.
278.
Jaimey D. Tucker C. Alistair Siebert Maryana Escalante Peter G. Adams John D. Olsen Cees Otto David L. Stokes C. Neil Hunter 《Molecular microbiology》2010,76(4):833-847
The purple phototrophic bacteria synthesize an extensive system of intracytoplasmic membranes (ICM) in order to increase the surface area for absorbing and utilizing solar energy. Rhodobacter sphaeroides cells contain curved membrane invaginations. In order to study the biogenesis of ICM in this bacterium mature (ICM) and precursor (upper pigmented band – UPB) membranes were purified and compared at the single membrane level using electron, atomic force and fluorescence microscopy, revealing fundamental differences in their morphology, protein organization and function. Cryo‐electron tomography demonstrates the complexity of the ICM of Rba. sphaeroides. Some ICM vesicles have no connection with other structures, others are found nearer to the cytoplasmic membrane (CM), often forming interconnected structures that retain a connection to the CM, and possibly having access to the periplasmic space. Near‐spherical single invaginations are also observed, still attached to the CM by a ‘neck’. Small indents of the CM are also seen, which are proposed to give rise to the UPB precursor membranes upon cell disruption. ‘Free‐living’ ICM vesicles, which possess all the machinery for converting light energy into ATP, can be regarded as bacterial membrane organelles. 相似文献
279.
María Escalante‐Pérez Ales Svatos Jorge Molina‐Torres Alexander Muck Enrique Ramirez‐Chávez Rosa‐María Ádame‐Alvarez Martin Heil 《The Plant journal : for cell and molecular biology》2013,73(4):546-554
Despite the ecological and evolutionary importance of nectar, mechanisms controlling its synthesis and secretion remain largely unknown. It is widely believed that nectar is ‘secreted phloem sap’, but current research reveals a biochemical complexity that is unlikely to stem directly from the phloem. We used the short daily peak in production of extrafloral nectar by Acacia cornigera to investigate metabolic and proteomic dynamics before, during and after 2 h of diurnal secretion. Neither hexoses nor dominating nectar proteins (nectarins) were detected in the phloem before or during nectar secretion, excluding the phloem as the direct source of major nectar components. Enzymes involved in the anabolism of sugars, amino acids, proteins, and nectarins, such as invertase, β–1,3–glucanase and thaumatin‐like protein, accumulated in the nectary directly before secretion and diminished quantitatively after the daily secretion process. The corresponding genes were expressed almost exclusively in nectaries. By contrast, protein catabolic enzymes were mainly present and active after the secretion peak, and may function in termination of the secretion process. Thus the metabolic machinery for extrafloral nectar production is synthesized and active during secretion and degraded thereafter. Knowing the key enzymes involved and the spatio‐temporal patterns in their expression will allow elucidation of mechanisms by which plants control nectar quality and quantity. 相似文献
280.
Alfredo Escalante Ana GonçalvesAase Bodin Agnes StepanCorine Sandström Guillermo Toriz Paul Gatenholm 《Carbohydrate polymers》2012,87(4):2381-2387
Arabinoglucuronoxylan was extracted from Norway spruce and films prepared by casting from aqueous solution. The sugar analysis and NMR confirmed that the spruce xylan was composed of arabinose, 4-O-methyl-glucuronic acid and xylose in a ratio of 1:2:11 respectively. Substitutions of 4-O-methyl-α-d-GlcpA at O2 and of α-l-Araf at O3 on the xylose backbone were found by NOE analysis. NOE cross-peaks indicated as well that there is at least one free xylose on the main chain present between two substitutions. Whether the distribution of side chains was random or in blocks was uncertain. The average molecular weight of the sample was determined by size exclusion chromatography to be 12,780 g/mol. Arabinoglucoronoxylan casting yielded transparent flexible films with an average stress at break of 55 MPa, strain at break of 2.7% and a Young's Modulus 2735 MPa. Wide-angle X-ray scattering analysis showed that the arabinoglucuronoxylan films were totally amorphous. Addition of sorbitol as plasticizer resulted in less strong but more flexible films (strain at break of 5%). Peaks of crystallinity could be seen in X-ray which corresponds to sorbitol crystallizing in distinct phases. The dynamic mechanical analysis showed that the arabinoglucuronoxylan film softened at a later relative humidity (80% RH) in comparison with plasticized films (60% RH). The films showed low oxygen permeability and thus have a potential application in food packaging. 相似文献