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81.
Maria Ericsson Rie Henriksen Johan Bélteky Ann-Sofie Sundman Kiseko Shionoya Per Jensen 《PloS one》2016,11(4)
Stress in animals causes not only immediate reactions, but may affect their biology for long periods, even across generations. Particular interest has been paid to perinatal stress, but also adolescence has been shown to be a sensitive period in mammals. So far, no systematic study has been performed of the relative importance of stress encountered during different life phases. In this study, groups of chickens were exposed to a six-day period of repeated stress during three different life phases: early (two weeks), early puberty (eight weeks) and late puberty (17 weeks), and the effects were compared to an unstressed control group. The short-term effects were assessed by behaviour, and the long-term and transgenerational effects were determined by effects on behavior and corticosterone secretion, as well as on hypothalamic gene expression. Short-term effects were strongest in the two week group and the eight week group, whereas long-term and transgenerational effects were detected in all three stress groups. However, stress at different ages affected different aspects of the biology of the chickens, and it was not possible to determine a particularly sensitive life phase. The results show that stress during puberty appears to be at least equally critical as the previously studied early life phase. These findings may have important implications for animal welfare in egg production, since laying hens are often exposed to stress during the three periods pinpointed here. 相似文献
82.
Summary The fine structure of the secretory tubules in the kidney of the aglomerular goose-fish (Lophius piscatorius) is described. The cells have a pyramidal shape, are joined together by multiple desmosomes, and share as main characteristics: abundant and deep inflections of the basal and lateral cell membranes; coated luminal plasma membranes forming multiple microvilli or a genuine brush border; moderate numbers of comparatively small mitochondria, usually unassociated with the basal and lateral plasma membrane specializations; numerous multivesicular bodies occuring in the apical cytoplasm; abundant large lysosome-like bodies in the intermediate regions of the cytoplasm; and comparatively poor development of endoplasmic reticulum and Golgi apparatus.The observations suggest that the cells perform both absorptive and secretory functions and are metabolically unusually active in autolytic and heterolytic work. Comparisons with other aglomerular species indicate that the ability for active secretory function is not necessarily dependent on a close association between plasma membrane and mitochondria; however, this ability does appear to require a markedly increased basal and/or lateral cell surface created by multiple invaginations of the plasma membrane. The abundance of desmosomes and associated structures appears to represent a unique structural specialization of the goosefish tubule, and indicates that the cells must be firmly anchored to one another to supply a rigid and mechanically continuous lining of the tubule. The multivesicular bodies probably represent endocytic vacuoles which fuse with apical vesicles and invaginate their outer membrane to form the internal vesicles; they appear to transform to ambilysosomes via a function as heterophagosomes and — later — combined hetero- and autophagosomes.Supported by grants from Karolinska Institutet, Fonden til Videnskabens Fremme and Konsul Johannes Fogh-Nielsen og fru Ella Fogh-Nielsens Légat. Part of the study was performed at the Zoological Station at Naples, Italy. The assistance of Mrs. Britt-Marie Karlsson is gratefully acknowledged. 相似文献
83.
Summary The fine structural localization of acid phosphatase was studied in osteoblasts and osteocytes of fracture callus in the rat using glutaraldehyde-fixed EDTA-decalcified, dimethylsulfoxide-treated tissues incubated in a modified Gomori lead salt medium. The results showed that enzyme was not only localized in conventional lysosomes but also in Golgi cisternae, Golgi associated vesicles, and — in the case of osteoblasts — GERL-like regions. The Golgi regions were large and abundant in osteoblasts and small and inconspicuous in osteocytes while lysosomes were of approximately equal size in the two cell types but appeared to be more concentrated in osteocytes. The results were discussed in relation to the possible role of lysosomes and lysosomal enzymes in osteocytic osteolysis and the functional diversity of the Golgi apparatus in osteogenic cells. 相似文献
84.
In counting internal helminthic parasites (the "worm burden") of domestic animals, physical restrictions often lead to sampling by small aliquots of unequal size among affected organs, among animals treated alike, and among groups of animals treated differently. We assess the impact of that type of sampling on the precision of the analyzed variable (log of estimated worm burden), derive the variance of the standard nonlinear estimator of efficacy of anthelmintic treatment, and examine the problem of number of animals required for adequate sensitivity of experiments. The standard error of sample geometric mean worm burden, for a particular anthelmintic treatment, and the standard error of estimated efficacy of a treatment, relative to control, are given for the case of log-normal burdens. Small aliquots affect precision critically only if mean burden is small, i.e., when sampling by small aliquots is unnecessary, because the physical effort required is not great. The minimal number of animals per treatment, required for at least 80% power to detect efficacy of .7 or higher, is about 4 to 6 for species of parasites constituting major burdens (where the coefficient of variation of worm burden often is near .7). However, the minimal number of animals may be as high as 15 to 20 per treatment for cases with lowly-abundant species of parasites (where the coefficient of variation may be as high as 2 or 3). An example is given to illustrate procedures. 相似文献
85.
Incidence of bacterial enteropathogens in foods from Mexico 总被引:2,自引:0,他引:2
L V Wood L E Ferguson P Hogan D Thurman D R Morgan H L DuPont C D Ericsson 《Applied and environmental microbiology》1983,46(2):328-332
We examined food consumption patterns of U.S. students temporarily living in Guadalajara, Mexico. Consumption of foods prepared in Mexican homes was associated with an increased risk of acquisition of diarrhea. Foods from commercial sources and private Mexican homes in Guadalajara were subsequently examined for contamination with coliforms, fecal coliforms, and bacterial enteropathogens. For comparison, selected restaurant foods were obtained in Houston, Tex. Food obtained from Mexican homes showed generally higher counts of coliforms and fecal coliforms than those obtained from commercial sources in Mexico and Houston. The foods in Mexico, both from homes and commercial sources, commonly contained Escherichia coli and occasionally enterotoxigenic E. coli. Foods in Houston were not contaminated with E. coli or enterotoxigenic E. coli. Salmonella (17 isolates), Shigella (4 isolates), and Aeromonas hydrophila (1 isolate) were found only in the foods obtained from Mexican homes. Enterotoxigenic non-E. coli Enterobacteriaceae was recovered with approximately equal frequency from all food sources. 相似文献
86.
Primary structure of yeast cytochrome c peroxidase. II. The complete amino acid sequence 总被引:1,自引:0,他引:1
K Takio K Titani L H Ericsson T Yonetani 《Archives of biochemistry and biophysics》1980,203(2):615-629
The complete amino acid sequence of 293 residues in the single polypeptide chain of yeast cytochrome c peroxidase has been determined. Sequence analyses were performed on fragments obtained by cleavage with cyanogen bromide and by tryptic digestion of citraconylated protein. These fragments were aligned with sequential and compositional data as well as those obtained with intact protein, tryptic, and chymotryptic peptides (Takio and Yonetani, 1980, Arch. Biochem. Biophys.203, 605–614). Residues critical for catalysis by the enzyme were identified as arginine 48, tryptophan 51, histidine 52, and histidine 174 by fitting the sequence to the electron density map derived by others. Sequence comparison with other proteins show limited homology with horseradish peroxidase and myoglobin. 相似文献
87.
Tom Ericsson 《Physiologia plantarum》1981,51(4):423-429
The ability of Salix aquatica Smith, S. fragilis L. and S. viminalis L. to absorb nitrogen at varied growth rates was investigated in nutrient solutions. The effects of five nitrogen addition rates on growth rates, nitrogen contents of the plants, nitrogen concentration of the media, and dry matter distribution between roots, stems and leaves, were studied.
These clones are highly efficient in absorbing nitrogen at low concentrations in the root medium, independent of growth rate. Strong linear regressions were found between any two of the three factors: relative rate of nitrogen supply (RN ), nitrogen content (plant), and relative growth rate (R). Dry matter production per unit nitrogen taken up and unit time (N-productivity) and per unit nitrogen taken up independent of time (N-efficiency) was closely correlated to the nitrogen status of the plant. Root development was favoured in the sub-optimum treatments, and leaf development in the optimum treatments. With regard to their ability to absorb nitrogen, these Salix clones appear satisfactory for efficient short rotation energy forestry. 相似文献
These clones are highly efficient in absorbing nitrogen at low concentrations in the root medium, independent of growth rate. Strong linear regressions were found between any two of the three factors: relative rate of nitrogen supply (R
88.
89.
Bovine factor X 1a (activated Stuart factor). Evidence of homology with mammalian serine proteases 总被引:10,自引:0,他引:10
K Titani M A Hermodson K Fujikawa L H Ericsson K A Walsh H Neurath E W Davie 《Biochemistry》1972,11(26):4899-4903
90.