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排序方式: 共有258条查询结果,搜索用时 31 毫秒
251.
A I Svirnovski? T V Shimanskaia V G Tsygankov S N Cherenkevich V I Levin 《Tsitologiia》1988,30(3):321-326
A substance inhibiting DNA synthesis in mouse leukemic cells was isolated from the regenerating calf spleen. When added to a suspension of leukaemic cells, this substance is adsorbed on their surface. The following changes in cell features being noticed: 1) a minute decrease in electrophoretic cell motility, 2) a decrease in esterase activity of the cells, 3) an increase in microviscosity of membrane lipids, 4) an increase in the intracellular pH values. With a longer contact with this substance, changes in nuclear chromatin structure were noticed, with special reference to weakened bonds between DNA and proteins. The data obtained are of significance for revealing molecular mechanisms of hematopoietic mediator action on target cells. 相似文献
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I. Serebrijski M. Gomelsky E. Bashkirova A. Chistoserdov Y. D. Tsygankov 《Molecular genetics and genomics : MGG》1998,258(1-2):133-138
We present a refined genetic map of the obligate methylotroph Methylobacillus flagellatum. New, Hfr (high-frequency-of-transfer) donors, and pulsed-field gel electrophoresis, were used to determine that M.?flagellatum contains one ~3.1-Mb circular chromosome, and no plasmids. A correlation between time-of-entry units and DNA length was established. Using in vivo and in vitro cloning, and sequencing, a number of new genetic markers were identified and mapped; in addition, the nature of some of the previously mapped markers was elucidated. 相似文献
254.
A Iu Tsygankov Iu A Motorin A E Dobrushkin A D Vol'fson A F Orlovski? 《Biokhimii?a (Moscow, Russia)》1986,51(4):590-595
The effect of polymers (proteins, polyaminoacids, polyethylenimine) on kinetic parameters of lactate dehydrogenase (LDH) from porcine skeletal muscle was studied. Activation of the enzyme which was partially due to the association of LDH dimers was observed. A hypothesis was proposed, according to which the contribution of dissociation of oligomeric enzymes in the regulation of their activity in vivo is negligible due to the equilibrium shift towards association in dissociable enzyme systems. 相似文献
255.
M. A. Tsygankov A. E. Zobnina M. V. Padkina 《Applied Biochemistry and Microbiology》2014,50(4):387-393
Genes encoding truncated versions of bovine and chicken gamma interferon genes lacking predicted protease cleavage sites at the C-terminus were constructed and expressed in the yeast Pichia pastoris. The recombinant proteins possessed increased stability in comparison with the corresponding wild-type gamma interferons while retaining biological activity. The recombinant strains provide a useful tool for the purification of bovine and chicken gamma interferons for their use in veterinary applications. 相似文献
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