FT Ⅰ, a novel positive myeloid-lineage-specific transcription regulatory element within the mouse myeloperoxidase gene enhancer, En 1

INTanDUCTI0NMyeloidcelldifferentiati0n,inwhichmultip0tentialprogenitorcellsarec0nvertedint00neofthesixmaturedifferentiatedcells,i.e.,erythr0cytes,platelets(megakary-ocytes),macr0phages,neutr0phils,e0sinophilsandbas0phils,involvestemporalre-gulati0nofexpression0fanumberoflineage-anddifferentiationstage-specificgenes.Understandingthedevel0pmentalspecificationoflineageaJswellasmaturationstageassociatedpatterns0fgeneexpressioninmyel0idcelldifferentiationrequiresanin-sightintothecontrol0findivid…     

新疆六个民族指纹白线的调查研究The hand Print of Six Nationalities in Xinjiang

本文首次报道了新家6各民族6134人（南3181人、女2953人）的指纹白线出现频率。结果表明,白线频率女高于男,民族间亦有差异,其中维吾尔、哈萨克、鸟孜别克族频率接近。塔吉克族居住高寒高原地区,属白色人种,频率明显低于以上3个民族,锡伯族和汉族生活习惯近似,且高于以上4个民族,作者认为,白线出现频率的高低与种族、性别、年龄和群体生长环境及生活习惯有关。We’ve studied 6134 individuals (male 3181, fimale 2953) of six nationalities in Xinjiang.The result is that the white line of hand print is associated with rae,sex,age,grow environments of groups and life habbits.The ifference of races is:The appearant rates of white line of Weivuerite,Hasakese and Wuzibese are near,Tajike’s rate is lower than xiboman’s and han are higher than others.     

原生质体诱变选育无孢平菇

用紫外线照射紫孢侧耳(Pleurotus sapidus)双核菌丝原生质体,再生后筛选生长势好的菌株, 通过出菇试验得到了生产性状与紫孢侧耳一致的无孢和少孢平菇新品种。Abstract: This paper reported isolation and regeneration of the dikaryocyte protoplasts from Pleurotus sapidus, and the protoplasts were treated by U.V-irradiation for selection spore less mutants.     

一种制备鱼类染色体的新方法

自1966年Ojima建立鱼类染色体空气干燥法以来,鱼类染色体研究技术有了很大进展.目前有肾细胞培养法、上皮细胞培养法和外周血培养法等。细胞培养需要在有一定条件的实验室进行(如无菌操作),因而简便快速的制备鱼类染色体方法便不断产生,有PHA+秋水仙碱注射法、秋水仙碱体外注射法和CoCl+秋水仙碱体外注射等方法.本文介绍一种适合于在野外条件下进行的小型鱼类和鱼苗染色体制片方法。     

罗非鱼TRAF3基因的表达及功能研究

肿瘤坏死因子受体(TNFR)相关因子3(TRAF3)是多种免疫途径中的关键调控因子。从尼罗罗非鱼(Oreochromis niloticus)中克隆获得了TRAF3基因, 命名为OnTRAF3(GeneBank No. MN258118), 该基因包含1个环指结构域、1个锌指结构域、1个卷曲螺旋和MATH结构域。多序列比对表明, OnTRAF3与其他已知的TRAF3蛋白具有高度的相似性, 尤其是MATH结构域。实时荧光定量PCR(qRT-PCR)分析显示, OnTRAF3在各组织中广泛分布, 且在脑、皮肤、肠和鳃中表达量较高。在无乳链球菌诱导后, 多个组织中的OnTRAF3表达量出现了不同程度的上调, 说明OnTRAF3参与了罗非鱼的抗菌免疫应答。亚细胞定位实验显示, OnTRAF3分布在HEK293的细胞质和细胞核中。此外, 荧光素酶报告基因实验结果显示, 野生型(WT)OnTRAF3可显著激活NF-κB信号, 而coiled-coil和MATH 结构域缺失后, 依然能够显著激活NF-κB 活性, 而RING 和Zinc缺失后, 该激活作用则明显减弱, 表明RING 和Zinc 结构域是OnTRAF3在免疫信号通路中行使功能的关键结构域。研究为探索TRAF3在罗非鱼免疫应答中的功能提供了重要的基础。     

Distribution and Significance of Nitric Oxide Synthase in Brain Tissues of Rats Exposed to Deltamethrin Insecticide

The distribution of nitric oxide synthase(NOS)in brain tissues of rats exposed to deltamethrininsecticide has been examined by histochemical NADPH-diaphorase staining techniques on frozen sec-tions.After injection of deltamethrin(12.5mg/kg,i.p.),a reproducible sequence of toxic signs ofhyperexcitability were elicited.The observation and image analysis showed that,within brain sec-tions of rats exposed to deltamethrin,the numbers and the total staining areas of the NOS positiveneurons were greatly increased,especially in cerebral cortex,hippocampal formation and paraventric-ular nucleus.In addition,the density of single neuron and the processes were also increased.The re-sults suggested that deltamethrin may induce the NOS expression or activate the NOS activity.TheNOS activation may involve in the chains responsible for the excitatory neurotoxicities induced bydeltamethrin.     

正常人各年龄组染色体着丝粒点(Cd)研究

本文运用本室改良的Cd-NOR银染技术对80例4个年龄组的正常中国人的Cd变化进行了较系统的研究, 结果表明:(1)正常人随年龄增加,Cd消失的频率、Cd变异及Cd-NOR融合频率也相应增加,特别是Ⅲ、Ⅳ组(中、老年组)增加的频率尤为显著;(2)首次对Cd消失的过程提出了独特的观点,即Cd消失首先表现为Cd变小, 随着变小程度的加大,最终导致Cd消失;(3)在本研究中首次观察到单个Cd的现象,作者认为是细胞分裂中期染色体着丝一分为二的延迟现象。各年龄组间单Cd出现频率无统计学差异,同一年龄组中,2号染色体和1号染色体上单Cd出现频率显著高于理论值;(4)随年龄增高,Cd各项观察值的增高在男性与女性间未见明显的差异。 Abstract:The Cd variation of human chromosome in four groups of different age has been investigated.The result shows that the frequencies of Cd disappearing,size variation and Cd-NOR fusion increased with the age rising,especially in the group of aged people.We suggest that the variation of Cd shows the size changes first,and then disappears completely.We also observed some cells in which a few chromosomes shows only a single Cd in centromeric region.Cd variation in different age groups has no significant difference between the male and the female.     

陆地棉的核型模式

陆地棉的核型公式为2n=4x=52=30m+20sm(4SAT)+2st(2SAT); 染色体绝对长度平均3.40μm; 臂比值最大与最小的分别是4.02和1.14,平均1.77;随体3对,分别位于第12、19和26对同源染色体的短臂上;最长与最短染色体的比值为2.36;其核型属于Stebbin的2B类型。 Abstract:Karyotype formula of Gossypium hirsutum L.was 2n=4s=52=30m+20m (4SAT)+2st (2SAT); and its absolute lengths of chromosomes averaged 3.40μm; arm ratio differed from 1.24 to 4.02,with the mean value of 1.77; three pairs of satellite chromosomes; the ratio of the longest to the shortest chromosomes was 2.36; and the karyotype belonged to 2B in Stebbin’s classification.     

食管癌病人运铁蛋白(Tf)和组特异性成份(Gc)亚型分布的研究

应用超薄层聚丙烯酰胺凝胶等电聚焦电泳法, 分析了潮汕地区216例无血缘关系、临床上诊断为食管癌的病人和216例健康人的运铁蛋白(Tf)亚型分布情况, 结果发现:食管癌病人组TfC1C1纯合子频率为0.2639,Tf*Cl基因频率为0.4745,显著低于正常人组(分别为0.4352和0.6227,均为P<0.0 01);同时,食管癌病人组TfC2C2纯合子频率为0.2278,Tf*C2基因频率为0. 4977,显著高于正常对照组(分别为0.1852,P<0.05,和0.3634,P<0.001)。应用超薄层聚丙烯酰胺凝胶等电聚焦结合免疫固定法,分析了潮汕地区21 7例无血缘关系的临床上诊断为食管癌病人和 217例健康人的组特异性成份(Gc)亚型的分布,发现两组间无显著性差异。