二龄草鱼脾脏、肝脏组织高表达甘露糖结合凝集素mRNA

Innate immunity is expected to be very important in fish. Mannose-bingding lectin (MBL) participates in the innate immune system as an activator of the complement system and as an opsonin after binding to certain carbohydrate structures on microorganisms. In this experiment, total mRNA was isolated from spleen, liver, gills, thymus, head kidney and kidney of adult and immature grass carp Ctenopharygodon idllus. The cDNA of MBL was obtained by RT-PCR using total mRNA from the spleen of carp as template. Such cDNA was labled with ^32p and used as probe for Northern analysis, and autoradiographic signals were quantified by densitometry analysis. The results showed that MBL was high expressed in the spleen and liver and low in gills, thymus, head kidney and kidney of adult grass carp, and MBL was much lower expressed in spleen and liver of immature grass carp than those of adult grass carp. The results might partially explain why immature grass carp are vulnerable to grass carp hemorrhage virus (GCHV) whereas adult grass carp are not.This suggested that MBL mav be an imoortant anti-GCHV factor [Acta Zoologica Sinica 50 (1): 137 - 140. 2004].     

葡萄酒中白藜芦醇的分析

采用HPLC测定了17种葡萄酒中白藜芦醇含量。干红葡萄酒中白藜芦醇的含量远高于干白葡萄酒。干红葡萄酒中白藜芦醇的含量与葡萄品种有关,同一葡萄品种的干红葡萄酒中白藜芦醇的含量与产地和工艺有关。     

重组腺相关病毒(rAAV)介导的人凝血因子Ⅸ高活性突变衍生物在血友病B基因治疗研究中的应用

采用定点诱变技术, 将R338A点突变引入人凝血因子Ⅸ基因, 并构建于AAV载体上, 以rHSV/AAV杂合辅助病毒系统介导制备rAAV-hFIX重组病毒, 然后, 经肌肉注射对血友病B小鼠进行治疗实验, 观察该突变基因在小鼠体内的表达、活性以及机体对该突变衍生物的免疫反应与治疗效果. 结果显示: (i)治疗小鼠体内可检测到hFIX-R338A突变衍生物的存在, 并持续15周以上; (ii)突变衍生物hFIX-R338A在小鼠血浆中的凝血活性达(34.2±5.23)%, 显著高于野生型Ⅸ因子凝血活性((14.27±3.4)%); (iii)治疗小鼠体内未检测到抗Ⅸ因子突变衍生物抗体的存在; (iv)未发现与治疗相关的局部及全身性毒副作用. 提示: 以AAV介导人凝血因子Ⅸ高活性突变衍生物hFIX-R338A基因治疗可能成为替代野生型Ⅸ因子进行血友病B基因治疗的一个更为有效的途径.     

尼日利亚螫毛果(Cnestis ferruginca)中一种具有清除自由基的活性化合物的分离鉴定

用二苯基苦基苯肼自由基薄层实验法（DPPH-TLC-ASSAY）,从尼日利亚螫毛果（Cnestis ferruginca)植物的甲醇提取物中发现了一种具有很强的清除自由基活性的物质;用柱色谱及反相制备柱色谱（RP-LPPLC）,制备出了该活性物质的纯品;用质谱（HR ESI-MS）,核磁共振氢谱（^1H0,碳谱（^13C,DEPT)。紫外光谱及化学方法对该物质进行了结构鉴定,鉴定结果为对苯酚基-6-O-反式咖啡酸基-β-D-吡喃葡萄糖甙。     

迟缓爱德华菌的外膜蛋白图谱及耐药性分析

分析 2 7株不同来源的迟缓爱德华菌 (Et)的外膜蛋白 (OMP) ,可分为A～H 8个型。 2 1株致病株与 6株非致病株有明显不同的图谱。致病株OMP条带多而深浓 ,并且相同来源的菌株有几乎一致的图谱。其中国内致病株以E型为主 ,与ATCC参考株相似。非致病株OMP条带则浅而稀疏 ,来源虽不同 ,但图谱极类似。另外 ,6株非致病株对磺胺、庆大、四环素等抗生素普遍耐药 ,而致病株除少数几株外 ,均对抗生素有不同程度的敏感     

皱纹盘鲍肠粘膜上皮的结构和功能

以透射电镜和扫描电镜观察、组织化学及酶活性测定等方法研究了皱纹盘鲍的肠和直肠,肠和直肠粘膜上皮由5种细胞组成。具微绒的柱状细胞呈现吸收细胞的超微结构特征,纤毛柱状细胞参与运输食物颗粒和粪便,Ⅰ型腺细胞具蛋白酶、非特异性酯酶和脂酶活性,以顶浆分泌形式分泌消化酶,Ⅱ型腺细胞分泌物可能与包裹粪便有关,状细胞分泌酸性粘多糖。体外酶活性分析表明肠和直肠粘膜上皮分别具有4种和3种植物多糖酶。     

Flt3配体的同源二聚体构建及其生物学特性

Flt3配体 (FL)是一种早期造血生长因子 ,与相应受体结合后 ,驱动受体二聚化 ,从而启动细胞内信号转导。体内 ,可溶性的FL主要以单体形式存在。将 2个相同的编码可溶性FL的基因片段 ,通过连接序列以首尾相连的方式构建成FL FL基因 ,并在毕氏酵母 (Pichiapastoris)中表达。SDS PAGE试验表明 ,FL FL融合蛋白 (rFL FL)分子量约为 4 3kD ,与预期相符 ,并为Western印迹试验所证实。骨髓祖细胞体外集落形成试验提示 ,rFL FL具有更强的生物学活性 ,约是单体FL(rhFL)的 10倍之多。另外 ,FL FL融合蛋白还能促进和延长饥饿的Raji细胞存活 ,与rhFL相比 ,也表现出较强的生物学功能。     

黄喉拟水龟的繁殖生物学研究

1998年4-11月对黄喉拟水龟的繁殖生物学进行了研究,在广东,黄喉拟水龟的产卵高峰期在5-6月,窝卵量1-7枚,平均2.5枚;卵重平均13.95g;长径平均4.34cm;短径平均2.23cm。卵重与卵长径呈正相关关系,在25-32℃、沙床含水量5-10%孵化条件下,孵化时间平衡73.8d,孵化率84.2%。初生稚龟均重9.75g。背甲长平均3.77cm;背甲宽平均2.91cm。稚龟体重与背甲长、宽呈极显著的正相关关系,稚龟经8-11月3个月生长,平均日增重0.149g。稚龟体重、背甲长、宽与日龄呈极显著的正相关关系。稚龟体重与背甲长宽也呈极显著的正相关关系。     

Activation of Na+ /H+ exchange on rat preadipocyte plasma membrane and its role in cell proliferation and differentiation

An in vitro cultured rat perirenal preadipocyte (PA) was established as a model system to investigate the role of the intracellular pH (pHi) and of the Na~ /H~ exchanger during PA proliferation and differentiation, pH sensitive probe, 2' ,7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein(BCECF), was employed to measure the pHi of PA and to determine the Na~ /H~ exchange activity. The results showed that there was Na~ /H~ exchange activity in the plasma membrane of PA, FCS stimulated DNA synthesis measured by ~3H-TdR incorporation, and the activation of Na~ /H~ exchanger resulted in phi increase (nearly 0.2 pH unit) within 2 min. Ethyl-isopropyl-amiloride (EIPA), a specific Na~ /H~ exchange inhibitor, inhibited Na~ /H~ exchange activity and DNA synthesis. In the absence of serum insulin did not stimulate DNA synthesis but did induce PA differentiation characterized by the appearance of adiposome in the cell and the enhancement of glycerol-3-phosphate dehydrogenase (G_3PDHase) activity. Meantime, insu     

ANTIPYRETIC ACTION OF DEXAMETHASONE ON EGTAZIC ACIDINDUCED FEVER IN RABBITS

本文用脑室灌注和Ｆｕｒａ２测定细胞内游离钙技术观察了地塞米松（ｄｅｘａｍｅｔｈａｓｏｎｅ,ＤＥＸ）对家兔乙二醇双（２氨基乙醚）四乙酸性发热效应和下丘脑细胞内游离钙浓度（［Ｃａ２＋］ｉ）的影响,借此深入探讨地塞米松解热作用的中枢机制。结果发现：脑室灌注乙二醇双（２氨基乙醚）四乙酸（０６ｎｍｏｌ）引起家兔结肠温度明显升高,静脉注射地塞米松（５ｍｇ／ｋｇ）显著抑制家兔乙二醇双（２氨基乙醚）四乙酸性发热,地塞米松（６０～１２０μｍｏｌ／Ｌ）并不影响下丘脑细胞内［Ｃａ２＋］ｉ,而事先脑室灌注抑制基因转录的放线菌素Ｄ（３ｎｍｏｌ）则完全取消了地塞米松对乙二醇双（２氨基乙醚）四乙酸性发热的解热作用。这些结果提示：地塞米松显著抑制家兔乙二醇双（２氨基乙醚）四乙酸性发热,其机制与地塞米松激活脑内某些基因的表达有关,而与下丘脑神经细胞跨膜钙离子流无关。