黄鳝二价体上随机引物原位DNA合成的探讨

采用随机引物,在黄鳝二价体上建立了氚标记引物原位DNA合成技术,并详细探索了该技术的实验条件。其结果显示黄鳝二价体上DNA延伸效果十分明显,银颗粒基本上是非随机性的相间排列,而且主要特征是基本稳定的,据此可以进行染色体识别。此外,还讨论了建立该技术的重要意义。 Abstract:We have developed a technique of random-primed tritium labeled DNA extension on the pachytene bivalents of rice-field eels.The bivalents were not homogeneously labeled with this technique and the extension signals were very apparent and relatively stable,and these can be applied to identify the individual bivalent.The significance to develop this technique has also been discussed in detail.     

福建畲族苯硫脲味盲频率的分析

我国畲族总人口约６６万,主要分布在福建、浙江等省,其中福建有３６万人,宁德地区占１７万人。我国已对２０多个民族的ＰＴＣ尝味能力进行了调查,其中包括浙江畲族〔１〕,但福建畲族未见报道。我们用阈值法〔２〕测定宁德地区民族中学学生的苯硫脲味盲频率,其中畲族...     

TIBETODUS,A NEW PYCNODONT FISH FROM CHANGTU

A complete series of vomerine teeth of pycnodontoidea has been foundfrom the vicinity of Changtu(Sikang).It represents the first record of vertebrateremains in the Tibetan highlands.Order PycnodontoideaFamily CyrodontidaeGenus Tibetodus,new genusDiagnosis as given for the type species Tibetodus gyrodoidesSpecies Tibetodus gyrodoides,new species.Material:A vomer with the teeth nearly complete.Field No.C.3340.     

内蒙古达斡尔族舌运动类型的遗传学研究

调查了内蒙古达斡尔族舌运动类型（卷舌、叠舌、翻舌、尖舌、三叶舌）,共480例（男239例,女241例）。研究结果显示：(1) 卷舌、叠舌、翻舌、尖舌、三叶舌出现率分别为80.42％、4.58％、31.25％、81.88％、31.46％;(2) 卷舌、三叶舌出现率存在性别间明显差异;(3) 卷舌基因与翻舌、三叶舌基因间存在互作关系,翻舌基因与叠舌、尖舌、三叶舌基因间存在互作关系;(4) 卷舌、叠舌、翻舌、三叶舌的出现率存在民族间或种族间差异。 Abstact:　A sample of 480 cases (239 males, 241 females) of Daur nationality in Inner Mongolia was investigated on 5 tongue moving types including rolling, folding, twisting, pointed and clover-leaf tongues. The results revealed as follows: The freqencies of rolling, folding, twisting, pointed and clover-leaf tongues were 80.42%, 4.58%, 31.25%, 81.88%, and 31.46%, respectively. Significant sexual difference was showed in the frequency of rolling and clover-leaf tongues. Furthermore, some genetic interaction occured between twisting tongue and the other 4 tongue moving types, rolling tongue and clover-leaf tongue, respectively. The 5 tongue moving types but pointed tongue showed national or racial difference.     

蛇岛蝮蛇消化道嗜银细胞的分布及形态学观察

应用Grimelius银染法对蛇岛蝮蛇Gloydius shedaoensis 消化道嗜银细胞的分布密度及形态进行研究.结果 表明,蛇岛蝮蛇的消化道中嗜银细胞分布广泛,见于其全长,主要分布于上皮细胞基部、上皮细胞之间、腺泡上皮细胞之间,也有少量位于固有膜内;胃贲门是嗜银细胞分布密度的高峰,胃体次之,食管分布密度最低;嗜银细胞形态多样,主要有锥体形、梭形、圆形和椭圆形,具有内、外分泌两种功能.     

小麦T型细胞质雄性不育系与相应保持系线粒体DNA的RAPD分析

利用RAPD技术对小麦T型细胞质雄性不育系75-3369A和相应保持系75-336 9B 的线粒体DNA(mtDNA)进行了81个单引物和14组双引物的多态性研究。结果有73个单引物和全部的双引物有扩增结果,表现出多态性的有10个单引物和一组双引物。 Abstract:Using 81 single-primers and 14 double-primers,T-type CMS(cytoplasmic male sterile)line 75-3369 A and is maintainer 75-3369B were studied by means of RAPD.73 single-primers and all double-primers gave amplified products in the experiment,among them 10 single-primers and one double-primer presented polymorphism.     

Angiotensin II receptor blocker as a novel therapy in acute lung injury induced by avian influenza A H5N1 virus infection in mouse

<正>Dear Editor,Since the first recognized case of human infection with avian influenza H5N1 virus in 1997,the worldwide spread and re-emergence of this highly pathogenic influenza virus have led to 694 human infection cases.Of these cases,402died from 2003 to 2015(http://www.who.int/influenza/human_animal_interface/EN_GIP_20150106CumulativeNumber H5N1cases.pdf?ua=1),with a morbidity rate of     

Rapid changes of microsatellite flanking sequence in the allopolyploidization of new synthesized hexaploid wheat

Polyploidy has been found to be very common inplants. Comparative genome studies have revealed thateven species that were considered as typical diploidsincluding maize[1], soybean[2], Arabidopsis[3] have un-dergone polyploidization during their evolution. Ge-nome polyploidization is a major force of evolutionthat affects genome size and gene copy number[4,5]. Polyploids can be formed via the duplication ofgenomes, either of the same genomes (autopolyploid)or of diverged genomes with homoe…     

Effects of clinorotation on COL1A1- EGFP gene expression

Itisnowwellestablishedthatspaceflightin-ducesbonelossafterlong-termexposuretomicro-gravity.Biochemicalstudiesshowedthatcontinuousboneloss[1,2]andmineralredistribution[3]inducedbyspaceflightwereassociatedwiththedecreasedos-teoblasticactivityanddifferentialfunction[4—6].Recentfindingssuggestthechangeintheexpressionofbone-formationrelatedgeneplaysanimportantroleintheprocessofdecreasedosteoblasticfunctionsin-ducedbyspaceflight.CollagentypeIexpressedthroughouttheprocessofosteoblasticproliferationa…