几种化学药剂对川西獐牙菜种子萌发的影响

川西獐牙菜(Swertia mussotii)属龙胆科(Gentianaceae)一年生草本植物,为高寒藏药,藏名蒂达,产于青海海拔3 600～3 800 m的高寒地区.植物以全草入药,有清热解毒、舒肝利胆之功效,专治黄疸性肝炎和病毒性肝炎.目前,川西獐牙菜仍处于野生状态,其野生资源远远不能满足医药生产和外贸出口的需求.     

中药复方保肝作用的组织化学、免疫组织化学研究

目的为观察活血化瘀类保肝中药复方对肝损伤的保肝作用,并探讨其抗肝纤维化作用机制.方法采用组织化学酶显示法和免疫组织化学方法,经图像分析系统定量测定对照组、模型组、预防组、治疗组和自然恢复组各组动物标本中琥珀酸脱氢酶,5-核苷酸酶及热休克蛋白70活性与表达程度.结果琥珀酸脱氢酶、5-核苷酸酶活性和热休克蛋白70的表达模型组与对照组比P<0.01;自然恢复组、预防组与模型组比P<0.01;治疗组与自然恢复组比P>0.05.结论活血化瘀类中药复方具有一定的保肝功效.肝星形细胞热休克蛋白70的表达是否提示细胞凋亡的发生,将有待进一步研究.     

白眉蝮蛇去整合素Adinbitor的基因克隆、表达及其部分生物学活性

为了寻找去整合素家族的新成员 ,研究其在抗血栓与抗肿瘤等方面的作用 ,从中国白眉蝮蛇毒腺中提取总RNA进行RT PCR扩增 ,获得了 2 19bp的白眉蝮蛇去整合素基因 .测序结果显示 ,其与韩国的同为蝮蛇去整合素的saxatilin的DNA序列同源性为 95 8% ,蛋白质序列同源性为 91 8% ,且蛋白质中含有去整合素的特征模体 RGD .将去整合素基因进行克隆、转化与诱导后 ,得到了该蛋白的可溶性高效表达 .经组氨酸亲和层析纯化 ,获得了分子量为 9kD的均质蛋白 ,并将其命名为adinbitor .活性测定结果显示 ,adinbitor能明显抑制由碱性成纤维细胞生长因子诱导的人脐静脉血管内皮细胞ECV30 4的增殖 ,诱导ECV30 4细胞发生凋亡 ,并且呈剂量依赖性方式抑制ADP诱导的人血小板聚集     

Preliminarily functional analysis of a cloned novel human

ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene--ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis andcontains a potential fusion domain. A full-length cDNA of ADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalian ADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268-Asp374) as immunogen. The result of immunohistochemical test on human testis showed that ADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of tes-tis-associated cells.     

Computational analysis and prediction for exons of PAC579 genomic sequence

To isolate the novel genes related to human hepatocellular carcinoma (HCC), we se-quenced P1-derived artificial chromosome PAC579 (D17S926 locus) mapped in the minimum LOH (loss of heterozygosity) deletion region of chromosome 17p13.3 in HCC. Four novel genes mapped in this genomic sequence area were isolated and cloned by wet-lab experiments, and the exons of these genes were located. 0-60 kb of this genomic sequence including the genes of interest was scanned with five different computational exon prediction programs as well as four splice site recognition programs. After analyzing and comparing the computationally predicted results with the wet-lab experiment results, some potential exons were predicted in the genomic sequence by using these programs.     

应用基因芯片技术检测肝组织的基因表达

为检测正常肝组织中的基因表达状况,采用cDNA microarray技术对正常肝组织中表达的1500个基因进行定量,结果为97个基因较高表达,1010个基因中度表达,380个基因低表达,结果是cDNA microarray技术是大规模检测基因表达的有效方法。     

肺炎链球菌在复发性阿弗他溃疡中定居部位的变化

目的探讨复发性阿弗他溃疡表面细菌的定居状态.方法 分别对病例组溃疡灶及对照组唇部粘膜进行了细菌学分离对比研究.结果 病例组溃疡灶以肺炎链球菌为主,而对照组唇部粘膜以甲型溶血性链球菌为主. 结论复发性阿弗他溃疡患者中肺炎链球菌定居部位发生改变.     

阴道分泌物显微镜检查与应用

阴道分泌物显微镜检查是诊断妇女外生殖道感染的最简单、最常用的检查手段。应用该方法可对念珠菌感染、阴道滴虫感染、细菌性阴道病 (Bacterial Vaginosis,BV)等病原体感染做出较明确诊断并指导治疗 ,还可对阴道内的菌群状况做出综合评价。1　阴道分泌物检查的常用方法1.1　生理盐水湿涂片法　生理盐水湿涂片用可于检出阴道滴虫和线索细胞 ,也用可于念珠菌检查。在生理盐水湿涂片中 ,阴道滴虫在镜下呈梨形或水滴状、无色透明或淡蓝绿色虫体 ,大小约 (10～ 30 )× (5～ 15 ) μm,稍大于白细胞。虫体借助于鞭毛和波动膜作螺旋式跳动 ,运动…     

长春市职业献血员丙型肝炎病毒感染状况的调查

为了解长春市职业献血员丙型肝炎病毒感染状况,对该群体进行了抗-HCV调查.结果表明,长春市职业献血员抗-HCV阳性率为9%,显著高于对照人群;献浆者抗-HCV阳性率显著高于献全血和非献血人群,而且随着献血年限、次数的增加,感染丙型肝炎机会也会增加.     

毛木耳多糖的提取及生物活性研究进展

从毛木耳多糖的提取、药理功能及免疫活性等方面对其研究现状进行综述,为毛木耳多糖的研究提供科学依据。