非同位素PCR-SSCP方法的初步临床应用

单链构象多态性检测法 (PCR-SSCP)是近年发展起来的一项检测人类基因组 突变的新技术。然而,在该技术中需要使用放射性同位素标记的核苷酸或引物,从而限制 了其广泛临床研究及诊断方面的应用。本文报告一种改进的PCR-SSCP方法, 该方法不用 同位素标记引物,而直接在溴乙啶染色的聚丙烯酰胺凝胶上显示SSCP。用该方法对55例 平滑肌肉瘤p53基因第7外显子突变的检测表明,38%的瘤组织DNA存在异常的SSCP。其中10例有HaeⅢ和MspⅠ酶切位点的突变(18%), 19例有突变型p53蛋白的过度表达(9例同时有异常SSCP改变)。而p53质粒D NA,平滑肌瘤及Alzheimer病患者基因组DNA无p53基因第7外显子扩增片段的异常SSCP改变。同时,还使用该方法对临床诊断的20例Alzheimer病患者和8例健康对照进行了β-淀粉样蛋白前体基因第16和17外显子的扩增及分析,均未发现有异常SSCP改变及EcoRⅠ,BclⅠ酶切位点的突变。本研究结果提示,该非同位素PCR-SSCP方法可靠、敏感、简便、快速,具有潜在的推广价值。     

黄鳝二价体上随机引物原位DNA合成的探讨

采用随机引物,在黄鳝二价体上建立了氚标记引物原位DNA合成技术,并详细探索了该技术的实验条件。其结果显示黄鳝二价体上DNA延伸效果十分明显,银颗粒基本上是非随机性的相间排列,而且主要特征是基本稳定的,据此可以进行染色体识别。此外,还讨论了建立该技术的重要意义。 Abstract:We have developed a technique of random-primed tritium labeled DNA extension on the pachytene bivalents of rice-field eels.The bivalents were not homogeneously labeled with this technique and the extension signals were very apparent and relatively stable,and these can be applied to identify the individual bivalent.The significance to develop this technique has also been discussed in detail.     

Functional expression of opioid receptor—like receptor and its endogenous specific agonist nociceptin／orphanin FQ during mouse embryogenesis

INTRODUCTIONOpioidreceptorsbelongtotheG-protein-coupledreceptorfamilythatischaracterizedbytheseventransmembranespanningdomainsinstructure.Threesubtypesoftheopioidreceptors(H,6,andK)havebeenclonedandcharacterizedthroughtheir1.CorrespondingauthorFunctionalexpressionofORLIandN/OFQduringmouseembryogenesisdistinctaffinitiesfordifferentopioidligands.TheseopioidreceptorsareallcoupledtotheinhibitoryGprotein(Gi)andnegativelyregulateadenylatecyclase[1].Opioidreceptor--likereceptor(ORLI),anew…     

一例母 JUE TI 及其亲子DNA指纹与血清蛋白、酯酶遗传的研究

采用DNA指纹分析和聚丙烯酰胺凝胶电泳法, 对一例与公驴交配生育了后代的母|JUE||TI|后代进行了亲缘鉴定和血清蛋白、酯酶遗传的分析。结果可以认定其亲子关系并证实母骡生育的事实。虽然本例母|JUE|| TI|的后代在Pr、Al、Pa和Hb、Es等基因座位上的基因表达倾向于驴,但其外貌仍明显地带有种间杂种的特征。因此,尚不能简单地认为其已“回归”为纯种的驴。 Abstract:By adopting DNA fingerprints analysis and polyacrylamide gel electrophoresis simultaneously,the author made an genetical study on analytical evaluation of kindred relationship and contents of serum protein and esterase of a female hinny and her offspring bred after her mating with a masculine ass.As a result,the parental relationship together with the child-bearing fact of the hinny is hereby clearly verified.The appearance of the offsping,however,still showed some prominent characters of interspecific hybrid,though in the gene demonstration the offsping was proved inclining to the ass especially on those gene loci as Pr,AL,Pa,Hb and Es.Therefore,in the author′s opinion,the offsping could not be simply regarded as a “regressed” ass pure-line.     

寻常型银屑病遗传度的研究

对临床已确诊的212例寻常型银屑病患者进行了一、二、三级亲属发病率调查, 结果表明,患者一级亲属的发病率为3.8%,对照组一级亲属发病率为0.42%,本病的遗传方式为多基因遗传,遗传度为58.2%。     

黄鳝二价染色体上地高辛配基标记随机引物原位DNA合成的研究

在黄鳝二价染色体上, 运用地高辛配基标记的随机引物原位DNA合成技术诱导出了明暗相间、基本稳定且类似于R带的带状结构。本文对该结果进行了较详细的分析和讨论。 Abstract:The dark bands alternating with light bands,relatively stable and R-band-like structure was showed on the pachytene bivalents of Rics-field eel(Monopterus albus Zuiew)by using the random-primed in situ digoxigenin-labelled DNA synthesis technique,and the results were analysed and discussed in detail.     

The first mitochondrial genome for the butterfly family Riodinidae(Abisara fylloides) and its systematic implications

The Riodinidae is one of the lepidopteran butterfly families. This study describes the complete mitochondrial genome of the butterfly species Abisara fylloides, the first mitochondrial genome of the Riodinidae family. The results show that the entire mitochondrial genome of A. fylloides is 15 301 bp in length, and contains 13 protein-coding genes, 2 ribosomal RNA genes, 22transfer RNA genes and a 423 bp A+T-rich region. The gene content, orientation and order are identical to the majority of other lepidopteran insects. Phylogenetic reconstruction was conducted using the concatenated 13 protein-coding gene(PCG) sequences of19 available butterfly species covering all the five butterfly families(Papilionidae, Nymphalidae, Peridae, Lycaenidae and Riodinidae). Both maximum likelihood and Bayesian inference analyses highly supported the monophyly of Lycaenidae+Riodinidae,which was standing as the sister of Nymphalidae. In addition, we propose that the riodinids be categorized into the family Lycaenidae as a subfamilial taxon.     

Effects of cyhalothrin on the transient outward potassium current in central neurons of Helicoverpa armigera

The effects of cyhalothrin on the transient outward potassium current in central neurons of Helicoverpa armigera were studied by using the patch clamp techniques. The results showed that before using cyhalothrin （10.5 mmol/L）, activation potential was approximately -40 mV, after application of the drug, the activation potential shifted roughly 10 mV to the negative potential direction, so channels can be activated more easily. Before and after cyhalothrin application, the change of current amplitude was insignificant. The value of V1/2 and k of activation curves did not change significantly, however, the V1/2 of the inactivation curves changed significantly. Inactivation curves significantly shifted to a negative direction, so that inactivation of the channels was hastened. It is indicated that there may exit a primary way in which cyhalothrin provides neurotoxicity to the nervous system through the regulation of activation potentials and inactivation state of IA channels.